Difference between revisions of "Team:NYU Abu Dhabi/Parts"

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<h6><b>Parts</b></h6>
 
<h6><b>Parts</b></h6>
 
<!-- <hr style="width:25%"> -->
 
<!-- <hr style="width:25%"> -->
 +
<br>
 +
<table>
 +
  <tr>
 +
    <th>Name</th>
 +
    <th>Type</th>
 +
    <th>Description</th>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808000">BBa_K2808000</a></td>
 +
    <td>DNA
 +
    </td>
 +
    <td>gbpA gene for the detection of Vibrio cholera</td>
 +
  </tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808001">
 +
BBa_K2808001
 +
</a></td>
 +
<td>DNA
 +
</td>
 +
<td>
 +
hipO gene for the detection of Campylobacter
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808002">
 +
BBa_K2808002
 +
</a></td>
 +
<td>DNA
 +
</td>
 +
<td>
 +
invA gene for the detection of Salmonella
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808003">
 +
BBa_K2808003
 +
</a></td>
 +
<td>DNA
 +
</td>
 +
<td>
 +
lmo0733 gene for the detection of Listeria
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808004">
 +
BBa_K2808004
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
gbpA F3 2
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808005">
 +
BBa_K2808005
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
gbpA B3 1
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808006">
 +
BBa_K2808006
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
Hipo PCR forward 1
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808007">
 +
BBa_K2808007
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
Hipo PCR reverse 1
  
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808008">
 +
BBa_K2808008
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
Hipo PCR forward 2
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808009">
 +
BBa_K2808009
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
Hipo PCR reverse 2
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808010">
 +
BBa_K2808010
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
PCR forward Salmonella 1
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808010">
 +
BBa_K2808010
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
PCR forward Salmonella 1
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808011">
 +
BBa_K2808011
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
PCR reverse Salmonella 2
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808012">
 +
BBa_K2808012
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
PCR forward Listeria
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808013">
 +
BBa_K2808013
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
lmo0733 B3 2
  
−
<div class="section-block">
+
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808014">
 +
BBa_K2808014
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
gbpA F3 1
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808015">
 +
BBa_K2808015
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
gbpA B3 1
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808016">
 +
BBa_K2808016
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
gbpA FIP 1
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808017">
 +
BBa_K2808017
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
gbpA BIP 1
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808018">
 +
BBa_K2808018
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
hipO F3
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808019">
 +
BBa_K2808019
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
hipO B3
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808020">
 +
BBa_K2808020
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
hipO FIP
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808021">
 +
BBa_K2808021
 +
</a></td>
 +
<td>DNA
 +
</td>
 +
<td>
 +
hipO BIP
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808022">
 +
BBa_K2808022
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
invA F3 1
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808023">
 +
BBa_K2808023
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
invA B3 1
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808024">
 +
BBa_K2808024
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
invA FIP 1
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808025">
 +
BBa_K2808025
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
invA BIP 1
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808026">
 +
BBa_K2808026
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
lmo0733 F3 2
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808027">
 +
BBa_K2808027
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
lmo0733 B3 2
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808028">
 +
BBa_K2808028
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
lmo0733 FIP 2
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808029">
 +
BBa_K2808029
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
lmo0733 BIP 2
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808030">
 +
BBa_K2808030
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
gbpA RPA F2
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808031">
 +
BBa_K2808031
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
gbpA RPA R2
 +
</td>
 +
</tr>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808032">
 +
BBa_K2808032
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
invA RPA F1
 +
</td>
 +
</tr> <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808033">
 +
BBa_K2808033
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
invA RPA R1
 +
</td>
 +
</tr> <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808034">
 +
BBa_K2808034
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
lmo0733 RPA F1
 +
</td>
 +
</tr> <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2808035">
 +
BBa_K2808035
 +
</a></td>
 +
<td>Primer
 +
</td>
 +
<td>
 +
lmo0733 RPA R1
 +
</td>
 +
</tr>
 +
</table>
  
−
<center>
+
<br><br>
−
    <div class="tab">
+
<h4><u>BioBricks PCR amplification</u></h4>
−
        <button class="tablinks" onclick="openTab(event, 'Key Achievments')" id="defaultOpen">Basic Parts</button>
+
<img src="https://static.igem.org/mediawiki/2018/9/9d/T--NYU_Abu_Dhabi--Parts1.png"width="300" class="center">
−
        <button class="tablinks2" onclick="openTab(event, 'biology')">Composite Parts</button>
+
<br>
−
        <button class="tablinks3" onclick="openTab(event, 'engineering')">Characterized Parts</button>
+
<h2><i>Figure 1: Agarose gel electrophoresis showing PCR amplification of: gbpA BioBrick <a href="http://parts.igem.org/Part:BBa_K2808000">BBA_K2808000</a>; hipO <a href="http://parts.igem.org/Part:BBa_K2808001">BBA_K2808001</a>; invA <a href="http://parts.igem.org/Part:BBa_K2808002">BBA_K2808002</a>; lmo0733 <a href="http://parts.igem.org/Part:BBa_K2808003">BBA_K2808003</a>. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) negative control for gbpA BioBrick; (Lane 4) hipO BioBrick; (Lane 5) negative control for hipO BioBrick; (Lane 6) invA BioBrick; (Lane 7) negative control for invA BioBrick; (Lane 8) lmo0733 BioBrick; (Lane 9) negative control for lmo0733 BioBrick. Lanes 2, 4, 6, and 8 show bands around 1019 bp, 203 bp, 818 bp, and 403 bp respectively as expected for the PCR products of the gbpA, hipO, invA, and lmo0733 BioBricks respectively.
−
 
+
</i></h2>
−
    </div>
+
<br>
−
</center>
+
  
 +
<h4><u>BioBricks double digestion reaction</u></h4>
 +
<img src="https://static.igem.org/mediawiki/2018/1/1b/T--NYU_Abu_Dhabi--Parts2.png"width="300" class="center">
 +
<br>
 +
<h2><i>Figure 1: Agarose gel electrophoresis showing double digestion of the BioBricks: gbpA <a href="http://parts.igem.org/Part:BBa_K2808000">BBA_K2808000</a>; hipO <a href="http://parts.igem.org/Part:BBa_K2808001">BBA_K2808001</a>; invA <a href="http://parts.igem.org/Part:BBa_K2808002">BBA_K2808002</a>; lmo0733 <a href="http://parts.igem.org/Part:BBa_K2808003">BBA_K2808003</a> with EcoRI and PstI. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) gbpA BioBrick digested with EcoRI and PstI. (Lane 4) hipO BioBrick; (Lane 5) hipO BioBrick digested with EcoRI and PstI; (Lane 6) invA BioBrick; (Lane 7) invA BioBrick digested with EcoRI and PstI; (Lane 8) lmo0733 BioBrick; (Lane 9) lmo0733 BioBrick digested with EcoRI and PstI. The lengths of the pSB1C3 backbone, gbpA gene, hipO gene, invA gene, and lmo0733 genes are 2070 bp, 1458 bp, 1152 bp, 2065 bp, and 510 bp respectively.
 +
</i></h2>
 
<br>
 
<br>
−
    <div id="Key Achievments" class="tabcontent">
 
−
        <article>
 
−
 
−
<center>
 
−
 
−
<h2> Here will go the basic parts </h2>
 
−
 
−
</center>
 
−
 
−
        </article>
 
−
    </div>
 
−
 
−
    <div id="biology" class="tabcontent">
 
−
        <article>
 
−
<center>
 
−
  <h2> Here will go the composite parts</h2>
 
−
</center>
 
−
        </article>
 
−
    </div>
 
−
 
−
    <div id="engineering" class="tabcontent">
 
−
        <article>
 
−
<center>
 
−
<h2> Here will go the Characterized Parts </h2>
 
−
</center>
 
−
        </article>
 
−
    </div>
 
−
 
−
</div>
 
−
 
  
 +
<h4><u>gbpA BioBrick double digestion reaction</u></h4>
  
 +
<img src="https://static.igem.org/mediawiki/2018/b/bf/T--NYU_Abu_Dhabi--Parts3.png" height="500" class="center">
 +
<br>
 +
<h2><i>Figure 1: Agarose gel electrophoresis showing double digestion of the gbpA BioBrick <a href="http://parts.igem.org/Part:BBa_K2808000">BBA_K2808000</a> (gbpA gene (1458 bp) inserted into the pSB1C3 backbone (2070 bp)) with EcoRI and PstI. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) gbpA BioBrick digested with EcoRI and PstI.
 +
</i></h2>
  
  
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           <a class="dropdown-item" href="https://2018.igem.org/Team:NYU_Abu_Dhabi/Safety">Safety</a>
 
           <a class="dropdown-item" href="https://2018.igem.org/Team:NYU_Abu_Dhabi/Safety">Safety</a>
 
           <a class="dropdown-item" href="https://2018.igem.org/Team:NYU_Abu_Dhabi/Interlab">Interlab</a>
 
           <a class="dropdown-item" href="https://2018.igem.org/Team:NYU_Abu_Dhabi/Interlab">Interlab</a>
−
        </div>
+
<a class="dropdown-item" href="https://2018.igem.org/Team:NYU_Abu_Dhabi/Medal_Requirments">Medal Requirements</a>
 +
</div>
 
       </li>
 
       </li>
  
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                  <a href="https://www.facebook.com/iGEMNYUAD/" target="_blank"><span class="fa fa-facebook"></span></a>
 
                  <a href="https://www.facebook.com/iGEMNYUAD/" target="_blank"><span class="fa fa-facebook"></span></a>
 
                  <a href="https://www.twitter.com/igemnyuad/" target="_blank"><span class="fa fa-twitter"></span></a>
 
                  <a href="https://www.twitter.com/igemnyuad/" target="_blank"><span class="fa fa-twitter"></span></a>
−
                  <a href="https://www.youtube.com/channel/UCQnySAKw67slX8T6r9bAcYw" target="_blank"><span class="fa fa-youtube"></span></a>
 
 
                  <a href="https://www.instagram.com/igem_nyuad/" target="_blank"><span class="fa fa-instagram"></span></a>
 
                  <a href="https://www.instagram.com/igem_nyuad/" target="_blank"><span class="fa fa-instagram"></span></a>
 
                </h3>
 
                </h3>

Revision as of 21:20, 6 October 2018

<!DOCTYPE html>

Parts

Name Type Description
BBa_K2808000 DNA gbpA gene for the detection of Vibrio cholera
BBa_K2808001 DNA hipO gene for the detection of Campylobacter
BBa_K2808002 DNA invA gene for the detection of Salmonella
BBa_K2808003 DNA lmo0733 gene for the detection of Listeria
BBa_K2808004 Primer gbpA F3 2
BBa_K2808005 Primer gbpA B3 1
BBa_K2808006 Primer Hipo PCR forward 1
BBa_K2808007 Primer Hipo PCR reverse 1
BBa_K2808008 Primer Hipo PCR forward 2
BBa_K2808009 Primer Hipo PCR reverse 2
BBa_K2808010 Primer PCR forward Salmonella 1
BBa_K2808010 Primer PCR forward Salmonella 1
BBa_K2808011 Primer PCR reverse Salmonella 2
BBa_K2808012 Primer PCR forward Listeria
BBa_K2808013 Primer lmo0733 B3 2
BBa_K2808014 Primer gbpA F3 1
BBa_K2808015 Primer gbpA B3 1
BBa_K2808016 Primer gbpA FIP 1
BBa_K2808017 Primer gbpA BIP 1
BBa_K2808018 Primer hipO F3
BBa_K2808019 Primer hipO B3
BBa_K2808020 Primer hipO FIP
BBa_K2808021 DNA hipO BIP
BBa_K2808022 Primer invA F3 1
BBa_K2808023 Primer invA B3 1
BBa_K2808024 Primer invA FIP 1
BBa_K2808025 Primer invA BIP 1
BBa_K2808026 Primer lmo0733 F3 2
BBa_K2808027 Primer lmo0733 B3 2
BBa_K2808028 Primer lmo0733 FIP 2
BBa_K2808029 Primer lmo0733 BIP 2
BBa_K2808030 Primer gbpA RPA F2
BBa_K2808031 Primer gbpA RPA R2
BBa_K2808032 Primer invA RPA F1
BBa_K2808033 Primer invA RPA R1
BBa_K2808034 Primer lmo0733 RPA F1
BBa_K2808035 Primer lmo0733 RPA R1


BioBricks PCR amplification


Figure 1: Agarose gel electrophoresis showing PCR amplification of: gbpA BioBrick BBA_K2808000; hipO BBA_K2808001; invA BBA_K2808002; lmo0733 BBA_K2808003. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) negative control for gbpA BioBrick; (Lane 4) hipO BioBrick; (Lane 5) negative control for hipO BioBrick; (Lane 6) invA BioBrick; (Lane 7) negative control for invA BioBrick; (Lane 8) lmo0733 BioBrick; (Lane 9) negative control for lmo0733 BioBrick. Lanes 2, 4, 6, and 8 show bands around 1019 bp, 203 bp, 818 bp, and 403 bp respectively as expected for the PCR products of the gbpA, hipO, invA, and lmo0733 BioBricks respectively.


BioBricks double digestion reaction


Figure 1: Agarose gel electrophoresis showing double digestion of the BioBricks: gbpA BBA_K2808000; hipO BBA_K2808001; invA BBA_K2808002; lmo0733 BBA_K2808003 with EcoRI and PstI. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) gbpA BioBrick digested with EcoRI and PstI. (Lane 4) hipO BioBrick; (Lane 5) hipO BioBrick digested with EcoRI and PstI; (Lane 6) invA BioBrick; (Lane 7) invA BioBrick digested with EcoRI and PstI; (Lane 8) lmo0733 BioBrick; (Lane 9) lmo0733 BioBrick digested with EcoRI and PstI. The lengths of the pSB1C3 backbone, gbpA gene, hipO gene, invA gene, and lmo0733 genes are 2070 bp, 1458 bp, 1152 bp, 2065 bp, and 510 bp respectively.


gbpA BioBrick double digestion reaction


Figure 1: Agarose gel electrophoresis showing double digestion of the gbpA BioBrick BBA_K2808000 (gbpA gene (1458 bp) inserted into the pSB1C3 backbone (2070 bp)) with EcoRI and PstI. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) gbpA BioBrick digested with EcoRI and PstI.