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<p class="text-center"><font size="3"> Q: How to select engineered strains in Bluepha?</font></p> | <p class="text-center"><font size="3"> Q: How to select engineered strains in Bluepha?</font></p> | ||
<p class="text-center"><font size="3"> A: Currently, we use a holamonas strain which survives in high salt level. This potentially prevents from contamination of other bacerium as they are not able to grow in such an extreme condition.</font></p> | <p class="text-center"><font size="3"> A: Currently, we use a holamonas strain which survives in high salt level. This potentially prevents from contamination of other bacerium as they are not able to grow in such an extreme condition.</font></p> | ||
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Revision as of 14:26, 11 October 2018
Human Practices
Overview
Our goal is to increase the oxygen carrying capacity of engineered bacteria. We expect to increase the yield of engineered bacteria. If realized, the development of fermentation industry can be promoted. In order to get more information about our project and guide our project, we went to Bluepha to do research and interview. We also hope that by participating in the iGEM competition, we can have an impact on our community and the people around us. So we launched a questionnaire in the name of BJRS, and through the promotion of BJRS public name, to investigate public attitudes and understanding of genetically modified foods. A total of 675 feedbacks were received, ranging from under 12 to over 65, covering a wide range of educational qualifications and jobs. The results show that the public's understanding of GM is still inadequate, and the phenomenon of blind resistance is serious. So we carried out a lecture on Synthetic biology in Beijing Jianhua Experimental School.
Integrated Human Practices
The goal of our project is to increase the oxygen carrying capacity of engineered bacteria. We expect to increase the yield of engineered bacteria. If realized, the development of fermentation industry can be promoted. In order to get more information about our project and guide our project, we went to Bluepha to do research and interview.
Bluepha is a bio fermentation company. Its main products are bio plastics. In the company, we practically experienced biological plastic products, and learned the fermentation tank and fermentation process, also, interviewed the employees. The following is the interview record.
Here Are Some Q&As
Q: What is the most pressing issue in fermetation process?
A: The biggest issue is disinfection, which acounts for almost half of the excutive costs in most circumstances. Contamination of other bacteria lead to waste and disposal of entire fernetautib cylinder.
Q: What are the other pressing issues?
A: Yield of engineered strain and downstream processing.
Q: What is the current way of oxygen supply?
A: Currently we bubble sterilised air to the cylinder and use blades to stir for homogenous distribution of air.
Q: Have you ever think of other ways to increase oxygen capacity?
A: Not as I know it. The oxygen solubility in water gives a upper limit for oxygen capacity.
Q: What is the current fermetation rate?
A: It usually takes two to three days for a typical cylinder to finish fermetation. The downstream processes cost at least another two to three days.
Q: What factors affect the process?
A: The main factor is the nature of the engineered strain we chosen. Other factors include the product we desire, and the medium we use.
Q: How to select engineered strains in Bluepha?
A: Currently, we use a holamonas strain which survives in high salt level. This potentially prevents from contamination of other bacerium as they are not able to grow in such an extreme condition.