siRCon - A siRNA Constructor
In our Project we introduce RNA interference (RNAi) and silencing with siRNAs as an alternative for CRISPR/Cas. To make use of our silencing vector system Tace, functional siRNA for prokaryotic organisms must be determined. Thus, we developed a siRNA construction tool, which can find possible siRNAs for a given gene sequence and calculate their gene silencing probability. It consists of the three modules RNAi, siRNA and check siRNA. Obtained siRNAs are perfectly compatible with our siRNA vector system. To the best of our knowledge, this is the first tool dedicated to predicting customized siRNA for the application in prokaryotes. This Python tool comes in two versions: a command line application and an easy-to-use graphical interface.
