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With the use of site-direted mutagenesis we wanted to improve the part of last years LiU iGEM team. This was done through removing the fusion protein and the unnecessary bases as shown in fig. 1. By doing this the fluorescent protein mNeonGreen (mNG) can be used as a reporter in a fully functional operone, with the use of the AraC/pBAD inducible system. | With the use of site-direted mutagenesis we wanted to improve the part of last years LiU iGEM team. This was done through removing the fusion protein and the unnecessary bases as shown in fig. 1. By doing this the fluorescent protein mNeonGreen (mNG) can be used as a reporter in a fully functional operone, with the use of the AraC/pBAD inducible system. | ||
</h4> | </h4> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/d/d1/T--Linkoping_Sweden--improve.jpg"></img> | ||
+ | <h4> Figure 1. Improve. </h4> | ||
</div> | </div> | ||
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Revision as of 10:16, 14 October 2018