Difference between revisions of "Team:Linkoping Sweden/Demonstrate"

Line 65: Line 65:
 
</h3>
 
</h3>
 
<img src="https://static.igem.org/mediawiki/parts/9/92/T--Linkoping_Sweden--expdesign.png"/>
 
<img src="https://static.igem.org/mediawiki/parts/9/92/T--Linkoping_Sweden--expdesign.png"/>
 +
<h4>Figure x. A simple explaination on how we archived our experimental results. The plasmids shown is the same as before, where A can be either of mNG-Aß1-42, EGFP-Aß1-42, a-synuclein-EGFP and Tau0N4R-EGFP. The concentrations used for inducing the chaperone plasmids was 0.25 mg/ml L-arabinose, 200 ng/ml tetracycline. For the substrate plasmids we used 0.5 mM IPTG, and the substrate was induced 30 mins after the chaperone plasmids.
 
<h3>  
 
<h3>  
 
Results for the mNG-Aß1-42 substrate protein
 
Results for the mNG-Aß1-42 substrate protein

Revision as of 16:58, 14 October 2018

LiU iGEM

Demonstrate

All work done on BBa_K2671420

Plasmids used during the characterization of our biobrick

Overview of our experimental design to archive the results shown below

Figure x. A simple explaination on how we archived our experimental results. The plasmids shown is the same as before, where A can be either of mNG-Aß1-42, EGFP-Aß1-42, a-synuclein-EGFP and Tau0N4R-EGFP. The concentrations used for inducing the chaperone plasmids was 0.25 mg/ml L-arabinose, 200 ng/ml tetracycline. For the substrate plasmids we used 0.5 mM IPTG, and the substrate was induced 30 mins after the chaperone plasmids.

Results for the mNG-Aß1-42 substrate protein

Results for the EGFP-Aß1-42 substrate protein

Results for the a-synuclein-EGFP substrate protein

Results for the Tau0N4R-EGFP substrate protein