TUST-iGEM 2018 team members are committed to the establishment of a wide range of diverse parts library. We have constructed a detection and degradation system for tetracycline and data and phenomena demonstrate that they are valid.

We use the Gibson assembly method to build our parts. This way we were able to generate our required parts in a fast and efficient way. Therefore, all of the parts we used are composite. The different basic parts comprising the composite parts are documented seperately, although they do not exist induvidually.

In the case of a constitutive expression, the promoter is included in the biobrick itself. For the inducible expression, we made use of a plasmid backbone containing a Tet-promoter ，a Lac-promoter and the LacI gene, which encodes for the Lac repressor. And we improve both the function and characterization of the some part to better express in our constructive chasis.

	Name	Type	Description	Designer	Length
	BBa_K2685000	Coding	Lip	LIN,WENXIN	1113
	BBa_K2685001	Coding	Mnp	LIN,WENXIN	1131
	BBa_K2685002	Coding	TetR	LIN,WENXIN	624
	BBa_K2685003	Regulatory	TetO	LIN,WENXIN	54
	BBa_K2685004	Coding	TetA	LIN,WENXIN	1191
	BBa_K2685005	Coding	φX174E	LIN,WENXIN	276
	BBa_K2685006	Composite	TetO connected with TetA	LIN,WENXIN	1245
	BBa_K2685007	Composite	Detecting parts	LIN,WENXIN	2600
	BBa_K2685008	Composite	Detecting parts without TetA	LIN,WENXIN	1456
	BBa_K2685009	Composite	Detecting parts of the GFP to change position with TetA	LIN,WENXIN	2689
	BBa_K2685010	Composite	PLac promoter with Lip	LIN,WENXIN	1248
	BBa_K2685011	Composite	PTet promoter with Lip and LacI	LIN,WENXIN	2335
	BBa_K2685012	Composite	PTet promoter with Mnp and LacI	LIN,WENXIN	2353
	BBa_K2685013	Composite	PTet promoter with Lip,Mnp and LacI	LIN,WENXIN	3478
	BBa_K2685014	Composite	PTet promoter with Lip,Mnp,Laccase and LacI	LIN,WENXIN	5032
	BBa_K2685015	Composite	pTet+Mnp+LacI+pLac+φX174E	LIN,WENXIN	2741
	BBa_K2685016	Composite	pTet+Lip+LacI+pLac+φX174E	LIN,WENXIN	2723
	BBa_K2685017	Composite	pTet+Lip+Mnp+LacI+pLac+φX174E	LIN,WENXIN	3866
	BBa_K2685018	Composite	pTet+Lip+Mnp+Laccase+LacI+pLac+φX174E	LIN,WENXIN	5420
	BBa_K2685019	Reporter	GFP	LIN,WENXIN	717
	BBa_K2685020	Regulatory	J23114	LIN,WENXIN	40
	BBa_K2685021	Regulatory	PTet	LIN,WENXIN	54

We use the Gibson assembly method to build our parts. This way we were able to generate our required parts in a fast and efficient way. Therefore, all of the parts we used are composite.

	Name	Type	Description	Designer	Length
	BBa_K2685006	Composite	TetO connected with TetA	LIN,WENXIN	1245
	BBa_K2685007	Composite	Detecting parts	LIN,WENXIN	2600
	BBa_K2685008	Composite	Detecting parts without TetA	LIN,WENXIN	1456
	BBa_K2685009	Composite	Detecting parts of the GFP to change position with TetA	LIN,WENXIN	2689
	BBa_K2685010	Composite	PLac promoter with Lip	LIN,WENXIN	1248
	BBa_K2685011	Composite	PTet promoter with Lip and LacI	LIN,WENXIN	2335
	BBa_K2685012	Composite	PTet promoter with Mnp and LacI	LIN,WENXIN	2353
	BBa_K2685013	Composite	PTet promoter with Lip,Mnp and LacI	LIN,WENXIN	3478
	BBa_K2685014	Composite	PTet promoter with Lip,Mnp,Laccase and LacI	LIN,WENXIN	5032
	BBa_K2685016	Composite	pTet+Lip+LacI+pLac+φX174E	LIN,WENXIN	2723
	BBa_K2685017	Composite	pTet+Lip+Mnp+LacI+pLac+φX174E	LIN,WENXIN	3866
	BBa_K2685018	Composite	pTet+Lip+Mnp+Laccase+LacI+pLac+φX174E	LIN,WENXIN	5420

The different basic parts comprising the synthesized parts we used are documented here. Please note that some of these parts do not exist individually. And we improve both the function and characterization of the some part to better express in our constructive chasis.

	Name	Type	Description	Designer	Length
	BBa_K2685000	Coding	Lip	LIN,WENXIN	1113
	BBa_K2685001	Coding	Mnp	LIN,WENXIN	1131
	BBa_K2685002	Coding	TetR	LIN,WENXIN	624
	BBa_K2685003	Regulatory	TetO	LIN,WENXIN	54
	BBa_K2685004	Coding	TetA	LIN,WENXIN	1191
	BBa_K2685005	Coding	φX174E	LIN,WENXIN	276
	BBa_K2685019	Reporter	GFP	LIN,WENXIN	717
	BBa_K2685020	Regulatory	J23114	LIN,WENXIN	40
	BBa_K2685021	Regulatory	PTet	LIN,WENXIN	54