Difference between revisions of "Team:SHSBNU China/Safety"

Line 19: Line 19:
 
<div style="height:5vw;" class="first_classfication" onmouseover="fcova()" onmouseout="fcoua()" id="fc_Project"><!--Project-->
 
<div style="height:5vw;" class="first_classfication" onmouseover="fcova()" onmouseout="fcoua()" id="fc_Project"><!--Project-->
 
<div class="fc_box">
 
<div class="fc_box">
−
<a class="fc_link" style="height:100%; width:100%;" href="https://2018.igem.org/Team:SHSBNU_China/Background">
+
<a class="fc_link" style="height:100%; width:100%;" href="https://2018.igem.org/Team:SHSBNU_China/Project">
 
<h4 class="fc_content">Project</h4>
 
<h4 class="fc_content">Project</h4>
 
</a>
 
</a>
Line 27: Line 27:
 
<div style="height:5vw;" class="first_classfication" onmouseover="fcovb()" onmouseout="fcoub()" id="fc_Experiment"><!--Experiment-->
 
<div style="height:5vw;" class="first_classfication" onmouseover="fcovb()" onmouseout="fcoub()" id="fc_Experiment"><!--Experiment-->
 
<div class="fc_box">
 
<div class="fc_box">
−
<a class="fc_link" style="height:100%; width:100%;" href="https://2018.igem.org/Team:SHSBNU_China/Experiment">
+
<a class="fc_link" style="height:100%; width:100%;" href="https://2018.igem.org/Team:SHSBNU_China/Experiments">
 
<h4 class="fc_content">Experiment</h4>
 
<h4 class="fc_content">Experiment</h4>
 
</a>
 
</a>
Line 90: Line 90:
  
 
<div id="menu">
 
<div id="menu">
−
<p style="font-family: Serif;font-size: 3.5vw; font-weight: bold;text-align: center;margin: 0.5vw 0vw 0vw 0vw">Sample</p>
+
<p style="font-family: Serif;font-size: 3.5vw; font-weight: bold;text-align: center;margin: 0.5vw 0vw 0.5vw 0vw">Sample</p>
 
<div class="second_classfication">
 
<div class="second_classfication">
 
<a class="snd_class" href="">SHSBNU_China</a>
 
<a class="snd_class" href="">SHSBNU_China</a>
Line 103: Line 103:
 
<div class="third_classfication">
 
<div class="third_classfication">
 
<a class="trd_class" href="">classfication</a>
 
<a class="trd_class" href="">classfication</a>
 +
</div>
 +
<div id="menu_blank">
 
</div>
 
</div>
 
</div>
 
</div>
Line 108: Line 110:
 
<div id="articles">
 
<div id="articles">
 
<div id="sec_title">
 
<div id="sec_title">
−
<h1>Safety</h1>
+
<h1>Sample</h1>
 
</div>
 
</div>
  
 
<div id="sec_content">
 
<div id="sec_content">
−
<h2>I. OVERVIEW</h2>
+
<h2>I. Overview</h2>
−
<div class="content">
+
<div class="content">
−
+
<p class="text">
−
<section>
+
This year’s SHSBNU_China Team is trying to develop a highly efficient, low-cost and modularized biomaterial to decompose azo dye, the Biofilm x Laccase system, using the methods of synthetic biology. Here we wish to share our experiences with safety control.
−
<p class="text">
+
</p>
−
This year’s SHSBNU_China Team is trying to develop a highly efficient, low-cost and modularized biomaterial to decompose azo dye, the Biofilm x Laccase system, using the methods of synthetic biology. Here we wish to share our experiences with safety control.
+
</div>
−
</p>
+
−
</section>
+
−
</div>
+
  
−
<h2>II. SAFE PROJECT DESIGN</h2>
+
<h2>II. Safe Project Design</h2>
−
<div class="content">
+
<div class="content">
−
<section>
+
<p class="text">
−
<p class="text">
+
Biofilm base CotA operator, produced in this project, was secreted by genetically modified strains of host bacteria E. coli. As we developed a water-filter which prevents bacteria from leakage, the recombinant DNA will not flow out into the environment. Even if a small fraction of CotA-producing bacteria leaked out, it would not express any protein because of the shortage of IPTG. In addition, our filter structure contains sterilizing device which can keep biofilm from leakage. Thus, there is no doubt that our product is safe and reliable.
−
Biofilm base CotA operator, produced in this project, was secreted by genetically modified strains of host bacteria E. coli. As we developed a water-filter which prevents bacteria from leakage, the recombinant DNA will not flow out into the environment. Even if a small fraction of CotA-producing bacteria leaked out, it would not express any protein because of the shortage of IPTG. In addition, our filter structure contains sterilizing device which can keep biofilm from leakage. Thus, there is no doubt that our product is safe and reliable.
+
</p>
−
</p>
+
</div>
−
</section>
+
<h3>a. Safe Lab Work</h3>
 +
<div class="content">
 +
<p class="text">
 +
According to the requirements of iGEM policy, we performed safely in every experiment followed by some basic regulations as follows:
 +
</p>
 +
<p class="text">· Thorough experiment and regulations should be understood by participants.
 +
<p class="text">· Steps with any probability of contamination should be performed in a biosafety cabinet.
 +
<p class="text">· Duplicating a key to the laboratory without permission is strictly prohibited.
 +
<p class="text">· Wear rubber gloves during all experimenters.
 +
<p class="text">· Sanitize the lab using UV-light every day.
 +
<p class="text">· Fire, electric heaters, and microwave ovens should remain attended while operating.
 +
<p class="text">· All liquid and solid waste containing living organisms must be daily autoclaved.
 +
<p class="text">· The last person to leave the lab should make sure that water, electricity, gas, and air conditioners have been turned off, and doors and windows have been locked before leaving.
 +
</div>
  
−
<h3>a. SAFE LAB WORK</h3>
+
<h2>III. Safe Shipment</h2>
 +
<div class="content">
 +
<p class="text">
 +
The DNA parts are absolutely safe because they encode non-hazardous proteins such as SpyCatcher, SpyTag, biofilm, CotA and their combinations to human. The DNA parts were safely confined within PCR tubes as the Parts Registry requires.
 +
</p>
 +
</div>
  
−
<div style="float:left" class="content_pic_left">
 
−
<img class="pictures" id = "" src=""/>
 
−
<p class="pic_text"> 在这用 “不知道怎么命名 safety 1”
 
−
</p></div>
 
  
−
<div class="content">
 
−
<section>
 
−
<p class="text">
 
−
According to the requirements of iGEM policy, we performed safely in every experiment followed by some basic regulations as follows:<br/>
 
−
<li>Thorough experiment and regulations should be understood by participants. </li>
 
−
<li>Steps with any probability of contamination should be performed in a biosafety cabinet. </li>
 
−
<li>Duplicating a key to the laboratory without permission is strictly prohibited. </li>
 
−
<li>Wear rubber gloves during all experimenters. </li>
 
  
−
<div style="float:" class="content_pic_right">
 
−
<img class="pictures" id = "" src=""/>
 
−
<p class="pic_text">在这用 “不知道怎么命名 safety 2”</p></div>
 
−
 
−
<li>Sanitize the lab using UV-light every day. </li>
 
−
<li>Fire, electric heaters, and microwave ovens should remain attended while operating. </li>
 
−
<li>All liquid and solid waste containing living organisms must be daily autoclaved. </li>
 
−
<li>The last person to leave the lab should make sure that water, electricity, gas, and air conditioners have been turned off, and doors and windows have been locked before leaving. </li>
 
−
</p>
 
−
</section>
 
−
</div>
 
−
</div>
 
−
<h2>III.SAFE SHIPMENT</h2>
 
−
<div class="content">
 
−
<section>
 
−
<p class="text">
 
−
The DNA parts are absolutely safe because they encode non-hazardous proteins such as SpyCatcher, SpyTag, biofilm, CotA and their combinations to human. The DNA parts were safely confined within PCR tubes as the Parts Registry requires.
 
−
</p>
 
−
</section>
 
−
</div>
 
−
<!--这个就删了吧
 
  
 +
 +
 +
 +
 +
 +
 +
<h2>I. Sample</h2>
 +
<h3>a. Sample</h3>
 +
<div class="content">
 +
<div style="float:left" class="content_pic_left">
 +
<img class="pictures" id = "" src=""/>
 +
<p class="pic_text">This is a Picture. This is a Picture. This is a Picture. This is a Picture.</p>
 +
</div>
 +
<p class="text">
 +
This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. 
 +
</p>
 
<div style="float:" class="content_pic_right">
 
<div style="float:" class="content_pic_right">
 
<img class="pictures" id = "" src=""/>
 
<img class="pictures" id = "" src=""/>
 
<p class="pic_text">This is a Picture. This is a Picture. This is a Picture. This is a Picture.</p>
 
<p class="pic_text">This is a Picture. This is a Picture. This is a Picture. This is a Picture.</p>
 
</div>
 
</div>
−
 
−
 
−
 
 
<p class="text">
 
<p class="text">
 
This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture.   
 
This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture.   
 
</p>
 
</p>
−
-->
 
 
</div>
 
</div>
 
</div>
 
</div>
 
<div id=footer>
 
<div id=footer>
 
<div style="display:table-cell;vertical-align:middle;">
 
<div style="display:table-cell;vertical-align:middle;">
−
<p style="font-size:1vw;font-family:'Times New Roman';font-style:italic;color:#666666">SHSBNU_China 2018, Created by AzirQuantum</p>
+
<p style="font-size:1vw;font-family:'Times New Roman';font-style:italic;color:#666666">SHSBNU_China 2018, Created by Azir</p>
 
</div>
 
</div>
 
</div>
 
</div>

Revision as of 03:08, 16 October 2018

Section Sample

Sample

I. Overview

This year’s SHSBNU_China Team is trying to develop a highly efficient, low-cost and modularized biomaterial to decompose azo dye, the Biofilm x Laccase system, using the methods of synthetic biology. Here we wish to share our experiences with safety control.

II. Safe Project Design

Biofilm base CotA operator, produced in this project, was secreted by genetically modified strains of host bacteria E. coli. As we developed a water-filter which prevents bacteria from leakage, the recombinant DNA will not flow out into the environment. Even if a small fraction of CotA-producing bacteria leaked out, it would not express any protein because of the shortage of IPTG. In addition, our filter structure contains sterilizing device which can keep biofilm from leakage. Thus, there is no doubt that our product is safe and reliable.

a. Safe Lab Work

According to the requirements of iGEM policy, we performed safely in every experiment followed by some basic regulations as follows:

· Thorough experiment and regulations should be understood by participants.

· Steps with any probability of contamination should be performed in a biosafety cabinet.

· Duplicating a key to the laboratory without permission is strictly prohibited.

· Wear rubber gloves during all experimenters.

· Sanitize the lab using UV-light every day.

· Fire, electric heaters, and microwave ovens should remain attended while operating.

· All liquid and solid waste containing living organisms must be daily autoclaved.

· The last person to leave the lab should make sure that water, electricity, gas, and air conditioners have been turned off, and doors and windows have been locked before leaving.

III. Safe Shipment

The DNA parts are absolutely safe because they encode non-hazardous proteins such as SpyCatcher, SpyTag, biofilm, CotA and their combinations to human. The DNA parts were safely confined within PCR tubes as the Parts Registry requires.

I. Sample

a. Sample

This is a Picture. This is a Picture. This is a Picture. This is a Picture.

This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture.

This is a Picture. This is a Picture. This is a Picture. This is a Picture.

This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture. This is a Picture.