Difference between revisions of "Team:Austin UTexas/Parts"

Line 10: Line 10:
 
<br>
 
<br>
 
<h1>Parts</h1>
 
<h1>Parts</h1>
−
<br><br>
+
<br>
−
<h4>BBa_K2657001</h4>
+
<p><b>Overall, we submitted four basic Phytobricks and one composite Phytobrick</b></p>
 +
<br>
 +
<h4>BBa_K2657001 - Tetracycline Resistance Gene</h4>
 
<p>When observing the parts registry, we found that no tetracycline resistance gene had Phytobrick compatibility. Using primers, we added proper MoClo standard overhangs to each part as well as providing it the proper restriction sites — BsaI and BsmBI sites. The PCR products containing these overhangs were incorporated into the Universal Acceptor backbone, BBa_P10500, which is a modified pSB1C3 backbone. <a href = "http://parts.igem.org/Part:BBa_K2657001">Link to Part Page</a></p>
 
<p>When observing the parts registry, we found that no tetracycline resistance gene had Phytobrick compatibility. Using primers, we added proper MoClo standard overhangs to each part as well as providing it the proper restriction sites — BsaI and BsmBI sites. The PCR products containing these overhangs were incorporated into the Universal Acceptor backbone, BBa_P10500, which is a modified pSB1C3 backbone. <a href = "http://parts.igem.org/Part:BBa_K2657001">Link to Part Page</a></p>
 
<br><br>
 
<br><br>
−
<h4>BBa_K2657002</h4>
+
<h4>BBa_K2657002 - Rp4 Origin of Transfer</h4>
 
<p>At the same time while looking over the parts registry, we found no origin of transfer with Phytobrick overhangs. Using the same techniques that were used to make the TetR Phytobrick, we created a the Rp4 Phytobrick, which is a level 0 OriT Phytobrick.<a href = "http://parts.igem.org/Part:BBa_K2657002">Link to Part Page</a> Here is also an example of the Rp4 part used in conjugation <a href="https://2018.igem.org/Team:Austin_UTexas/Results/Conjugations">link</a></p>
 
<p>At the same time while looking over the parts registry, we found no origin of transfer with Phytobrick overhangs. Using the same techniques that were used to make the TetR Phytobrick, we created a the Rp4 Phytobrick, which is a level 0 OriT Phytobrick.<a href = "http://parts.igem.org/Part:BBa_K2657002">Link to Part Page</a> Here is also an example of the Rp4 part used in conjugation <a href="https://2018.igem.org/Team:Austin_UTexas/Results/Conjugations">link</a></p>
 +
<br><br>
 +
  
  

Revision as of 19:18, 15 October 2018


Parts


Overall, we submitted four basic Phytobricks and one composite Phytobrick


BBa_K2657001 - Tetracycline Resistance Gene

When observing the parts registry, we found that no tetracycline resistance gene had Phytobrick compatibility. Using primers, we added proper MoClo standard overhangs to each part as well as providing it the proper restriction sites — BsaI and BsmBI sites. The PCR products containing these overhangs were incorporated into the Universal Acceptor backbone, BBa_P10500, which is a modified pSB1C3 backbone. Link to Part Page



BBa_K2657002 - Rp4 Origin of Transfer

At the same time while looking over the parts registry, we found no origin of transfer with Phytobrick overhangs. Using the same techniques that were used to make the TetR Phytobrick, we created a the Rp4 Phytobrick, which is a level 0 OriT Phytobrick.Link to Part Page Here is also an example of the Rp4 part used in conjugation link



<groupparts>iGEM18 Austin_UTexas</groupparts>