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Revision as of 01:24, 16 October 2018
Integrated Human Practices
Optimization of Golden Gate Assembly
In order to increase the transformation efficiency of Golden Gate Assembly reactions we made standardized bridging sequences that reduced the number of parts in a reaction, increasing the likelihood of a successful assembly. Using this method, we were able to make many assemblies expressing different antibiotic resistance quickly.
Transformation of Assembly Plasmids into Mu Free Donors
Dr.Brian Redna of Gingko Bioworks emphasized that our kit would be limited by the ability of the plasmids to be inserted into the bacteria of interest as many can not be transformed with standard protocols. Therefore, we transformed the assembly plasmids into a strain of E. coli that can act as a plasmid donor in conjugations.
See Demonstrate Page
