Difference between revisions of "Team:William and Mary/Notebook"

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<li>Began cloning 3G parts based off the library sent from the Murray lab</li>
 
<li>Began cloning 3G parts based off the library sent from the Murray lab</li>
 
<li>Researched literature on dual inducible promoters</li>
 
<li>Researched literature on dual inducible promoters</li>
−
<li> Sent out several cloned 3G parts for sequencing</li>
+
<li>Sent out several cloned 3G parts for sequencing</li>
−
<li>   Planned out several IFFLs to test. </li>
+
<li> Planned out several IFFLs to test. </li>
 
</ul>
 
</ul>
 
<div>
 
<div>
 
   
 
   
 
<div style='padding-top: 40px;'></div>
 
<div style='padding-top: 40px;'></div>
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<div style = 'padding-left: 8%; padding-bottom: 10px;font-size: 25px' ><b> Week 2: 180604-180610: 180528-180604</b></div>
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<div style='background: #808080; margin: 0px 8% 20px 8%; height:2px;></div>
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<div style='padding-top: 0px;'></div>
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·        Continued cloning 3G parts onto our standard WM Pad backbone
 +
·        Redesigned primers for cloning 3G promoter parts, as previous ones were faulty
 +
·        Received results from sequencing, confirmed parts
 +
·        Beginning to assemble circuits using 3G assembly
 +
·        Making inducible reporter construct and an mf-Lon based IFFL
 +
 +
 +
<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 0px;line-height: 25px;'>
 +
<ul>
 +
<li>Continued cloning 3G parts onto our standard WM Pad backbone</li>
 +
<li> Redesigned primers for cloning 3G promoter parts, as previous ones were faulty</li>
 +
<li>Received results from sequencing, confirmed parts</li>
 +
<li>  Beginning to assemble circuits using 3G assembly</li>
 +
<li> Making inducible reporter construct and an mf-Lon based IFFL</li>
 +
</ul>
 +
<div>
 +
 +
<div style='padding-top: 40px;'></div>
 +
  
 
</body>
 
</body>

Revision as of 16:32, 16 October 2018

Notebook

Week 1: 180528-180604
  • Began cloning 3G parts based off the library sent from the Murray lab
  • Researched literature on dual inducible promoters
  • Sent out several cloned 3G parts for sequencing
  • Planned out several IFFLs to test.
Week 2: 180604-180610: 180528-180604
· Continued cloning 3G parts onto our standard WM Pad backbone · Redesigned primers for cloning 3G promoter parts, as previous ones were faulty · Received results from sequencing, confirmed parts · Beginning to assemble circuits using 3G assembly · Making inducible reporter construct and an mf-Lon based IFFL
  • Continued cloning 3G parts onto our standard WM Pad backbone
  • Redesigned primers for cloning 3G promoter parts, as previous ones were faulty
  • Received results from sequencing, confirmed parts
  • Beginning to assemble circuits using 3G assembly
  • Making inducible reporter construct and an mf-Lon based IFFL