Difference between revisions of "Team:William and Mary/Notebook"
Lillianparr (Talk | contribs) |
Lillianparr (Talk | contribs) |
||
| Line 48: | Line 48: | ||
<div style='padding-top: 40px;'></div> | <div style='padding-top: 40px;'></div> | ||
| − | <div style = 'padding-left: | + | <div style = 'padding-left: 8%; padding-bottom: 10px;font-size: 25px' ><b>Week 2</b></div> |
| − | <div style='background: #808080; margin: 0px | + | <div style='background: #808080; margin: 0px 8% 20px 8%; height:2px;></div> |
<div style='padding-top: 0px;'></div> | <div style='padding-top: 0px;'></div> | ||
Revision as of 16:44, 16 October 2018
Notebook
Week 1
- Began cloning 3G parts based off the library sent from the Murray lab
- Researched literature on dual inducible promoters
- Sent out several cloned 3G parts for sequencing
- Planned out several IFFLs to test.
Week 2
- Continued cloning 3G parts onto our standard WM Pad backbone
- Redesigned primers for cloning 3G promoter parts, as previous ones were faulty
- Received results from sequencing, confirmed parts
- Beginning to assemble circuits using 3G assembly
- Making inducible reporter construct and an mf-Lon based IFFL
Week 3: 180611-180617
- Completed cloning the portion of our comprehensive 3G assembly part library needed for our circuits
- Adding remaining 3G parts from Murray’s lab to our library
- Working on designing and building a dual inducible promoter and low copy backbone for assembly
- Confirmed minipreps based on sequencing results
- Started plate reader for experiments on circuits with IPTG and ATC inducer
- Started using FACS machine for experiments