Difference between revisions of "Team:UMaryland/History"

Revision as of 14:09, 17 October 2018

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Past iGEM Teams involved with PET

Listed here are past iGEM teams that have worked on projects on or related to PET degredation, with condensed descriptions of their results.

2012

BAU-Indonesia

Isolation of cutinase gene from nature with primers

TU Darmstadt

Surface display of cutinase on E. coli
Attempted TPA transport into E. coli, further research required
Expressed all TPH enzymes, did not attempt to measure activity
Confirmed anaerobic conversion of PCA via AroY and XylE enzymes

UC Davis

Confirmed cutinase activity using PNPB esterase assay
Engineered E. coli ethylene glycol metabolism with directed evolution

2013

Imperial College

Produced P3HB bioplastic from mixed waste containing at least some PET

2014

METU Turkey

Reduced catechol (downstream product) to pyruvate

ITB Indonesia

LC cutinatse activity confimed with SEM, PNPB

2015

Pasteur Paris

PNPB assay to confirm activity of esterase EST13
Fluorescent detection of TPA can not be accomplished when in LB broth

2016

ASIJ Tokyo

Attempt at detecting PET degradation by mass change failed

AUC Turkey

Withdrawn

Baltimore BioCrew

Planned to weigh PET degradation, no results

BGU Israel

PNPB and EM to confirm LC cutinase activity
P. putida can grow on PCA as sole carbon source, but not TPA
E. coli expressing LC-cutinase with pelB leader sequence grew on M9 plates with PET as sole carbon source. Expected to be due to consumption of ethylene glycol from PET degradation
Unable to determine enzyme efficiency based on growth due to heterogeneity in PET distribution
Measured fluorescence of TPA on plates, unable to quantify LC cutinase activity

Harvard BioDesign

Petase function confirmed with PNPB
Bacteria produced electric current when supplied with unspecified quantity of TPA

Tianjin

EM confirmation of PETase activity of PET film degradation
Multispectral scanning quantified PETase products for cell free system

TJUSLS China

HPLC detection of MHET to confirm PETase activity in varying conditions
Surface display of PETase in E. coli

UESTC-China

SEM and PNPB to confirm PETase activity
Possible detection of TPA by UV vis (higher absorbance across spectrum)

UoA New Zealand

Assembled PETase part with His tag

2017

Baltimore Bio-Crew

Fluorescine diacetate hydrolysis assay to confirm PETase and MHETase hydrolytic activity

BOKU-Vienna

Discussion of a possible method for directed evolution of PETase by culturing cells on PET film that fluoresces when degraded
ITB Indonesia

Successful biofilm formation on PET, but biofilm matrix hampered PETase activity
Engineered E. coli ethylene glycol metabolism with directed evolution

2018

Makerere University

TBA

OLS Canmore Canada

TBA

RHIT

TBA

ULaVerne

TBA

UMaryland

Check out our results on this wiki!

Yale

TBA

Contact Us
umarylandigem@gmail.com
Biology - Psychology Building
4094 Campus Dr, College Park, MD 20742

@@ Line 64: / Line 64: @@
 						<a href="https://2014.igem.org/Team:METU_Turkey">METU Turkey</a>
 						<ul>
-							<li>Reduced catechol to pyruvate</li>
+							<li>Reduced catechol (downstream product) to pyruvate</li>
 						</ul><br>
 						<a href="https://2014.igem.org/Team:ITB_Indonesia">ITB Indonesia</a>