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<h7><ins>Abstract</ins></h7> | <h7><ins>Abstract</ins></h7> | ||
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<h2>The team started with performing PCR for the plasmids as it is a general technique and the results would serve as a standard to compare the results of LAMP and RPA, the other relatively new amplification techniques we used for the project.</h2> | <h2>The team started with performing PCR for the plasmids as it is a general technique and the results would serve as a standard to compare the results of LAMP and RPA, the other relatively new amplification techniques we used for the project.</h2> | ||
<h2>We performed PCR, LAMP and RPA reactions to characterize our plasmids and determine if amplification happens with our designed primers. We wanted to test how specific each of these amplification techniques is by running each plasmid with its primers and the primers of other fragments. Our results show that LAMP is the most specific amplification technique which is consistent with results from the literature that show that LAMP has very high specificity (1). PCR had the lowest specificity which is also consistent with results from literature (2). RPA has been shown to be the best amplification technique currently available in terms of parameters like speed, complexity, and user-friendliness. However, our results show that RPA is less specific than LAMP. Daher et al (2015) (3) showed that mismatches can occur if extra precautions are not taken during primer design to eliminate this. In our case, our primers could be the reason for RPA being less specific than LAMP.</h2> | <h2>We performed PCR, LAMP and RPA reactions to characterize our plasmids and determine if amplification happens with our designed primers. We wanted to test how specific each of these amplification techniques is by running each plasmid with its primers and the primers of other fragments. Our results show that LAMP is the most specific amplification technique which is consistent with results from the literature that show that LAMP has very high specificity (1). PCR had the lowest specificity which is also consistent with results from literature (2). RPA has been shown to be the best amplification technique currently available in terms of parameters like speed, complexity, and user-friendliness. However, our results show that RPA is less specific than LAMP. Daher et al (2015) (3) showed that mismatches can occur if extra precautions are not taken during primer design to eliminate this. In our case, our primers could be the reason for RPA being less specific than LAMP.</h2> |
Revision as of 17:38, 17 October 2018