Difference between revisions of "Team:William and Mary/Parts"

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Revision as of 23:38, 17 October 2018

Parts

Parts

Besides our 3G parts, library we are submitting a series of heat inducible reporters(mScarlet) and protease(mf-Lon) which we are using to construct IFFLs with different parameters.

1. Heat inducible protease

Name
Part ID
K2680050 J23107 ts-CI + R0051 mf-Lon
K2680057 J23100 ts-CI + R0051 mf-Lon ssrA
K2680058 J23107 TPL + ptlpA BCD8 mf-lon thrL
K2680059 J23107 TPL + ptlpA BCD8 mf-lon ssrA thrL
K2680060 J23107 TPL + ptlpAr BCD8 mf-lon thrL
K2680061 J23107 TPL + ptlpAr BCD8 mf-lon ssrA thrL

2. Heat inducible reporters

Name
Part ID
K2680051 J23107 ts-CI + R0051 mScarlet-I pdt#3
K2680052 J23107 ts-CI + R0051 mScarlet-I pdt#3b
K2680053 J23100 ts-CI + R0051 mScarlet-I pdt#3
K2680054 J23100 ts-CI + R0051 mScarlet-I pdt#3b
K2680062 J23107 TPL + ptlpA b0034m mScarlet-I pdt#3
K2680065 J23107 TPL + ptplAr B0034 mScarlet-I pdt#3
K2680066 J23107 TPL + ptplA B0034 mScarlet-I pdt#3b
K2680067 J23107 TPL + ptplAr B0034 mScarlet-I pdt#3b
K2680068 J23107 ts-CI + R0051 mScarlet-I
K2680070 J23107 ts-CI + R0051 mScarlet-I pdt#3e
K2680071 J23107 TPL + ptlpA b0034m mScarlet-I pdt#3e
3. Heat inducible IFFLs
Name
Part ID
K2680064 J23107 TPL + ptlpA B0034m mScarlet-I pdt-3d thrL terminator + ptlpAr BCD8 mf-Lon ssrA
K2680069 J23107 TPL + ptlpA B0034m mScarlet-I thrL terminator + ptlpAr BCD8 mf-Lon ssrA
K2680063 J23107 TPL + ptlpA B0034m mScarlet-I pdt-3b thrL terminator + ptlpAr BCD8 mf-Lon ssrA