Difference between revisions of "Team:William and Mary/Parts"

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Besides our <a href='https://2018.igem.org/Team:William_and_Mary/Part_Collection' style="color:green;">3G parts</a>, library we are submitting a series of heat inducible reporters(mScarlet) and protease(mf-Lon) which we are using to construct IFFLs with different parameters.  
 
Besides our <a href='https://2018.igem.org/Team:William_and_Mary/Part_Collection' style="color:green;">3G parts</a>, library we are submitting a series of heat inducible reporters(mScarlet) and protease(mf-Lon) which we are using to construct IFFLs with different parameters.  
  
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<div class='row',align="center;", style="margin:50px; 10px; 0px; 0px;">
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3. Heat inducible IFFLs
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<table  style=' margin-left: 20%;'>
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<th style='background-color: #65A686;column-width: 100px;'><center>Name</center></th>
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<th style='background-color: #65A686;column-width: 800px;'><center>Part ID</center></th>
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<td style='background-color: #A2CBAB;column-width: 100px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680064"> K2680064 </a></td>
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<td style='background-color: #96B69C;column-width: 800px; font-size: 15px;'>J23107 TPL + ptlpA B0034m mScarlet-I pdt-3d thrL terminator + ptlpAr BCD8 mf-Lon ssrA</td>
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<td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680069"> K2680069 </a></td>
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<td style='background-color: #96B69C;column-width: 400px; font-size: 15px;'>J23107 TPL + ptlpA B0034m mScarlet-I thrL terminator + ptlpAr BCD8 mf-Lon ssrA</td>
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</tr>
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<tr>
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<td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680063"> K2680063 </a></td>
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<td style='background-color: #96B69C;column-width: 400px; font-size: 15px;'>J23107 TPL + ptlpA B0034m mScarlet-I pdt-3b thrL terminator + ptlpAr BCD8 mf-Lon ssrA</td>
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</table>
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</center>
 
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<div class='row',align="center;", style="margin:50px; 10px; 0px; 0px;">
 
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<center>
 
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3. Heat inducible IFFLs
 
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<table  style=' margin-left: 20%;'>
 
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<tr>
 
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<th style='background-color: #65A686;column-width: 100px;'><center>Name</center></th>
 
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<th style='background-color: #65A686;column-width: 800px;'><center>Part ID</center></th>
 
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</tr>
 
  
  
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<td style='background-color: #A2CBAB;column-width: 100px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680064"> K2680064 </a></td>
 
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<td style='background-color: #96B69C;column-width: 800px; font-size: 15px;'>J23107 TPL + ptlpA B0034m mScarlet-I pdt-3d thrL terminator + ptlpAr BCD8 mf-Lon ssrA</td>
 
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</tr>
 
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<tr>
 
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<td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680069"> K2680069 </a></td>
 
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<td style='background-color: #96B69C;column-width: 400px; font-size: 15px;'>J23107 TPL + ptlpA B0034m mScarlet-I thrL terminator + ptlpAr BCD8 mf-Lon ssrA</td>
 
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</tr>
 
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<tr>
 
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<td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680063"> K2680063 </a></td>
 
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<td style='background-color: #96B69C;column-width: 400px; font-size: 15px;'>J23107 TPL + ptlpA B0034m mScarlet-I pdt-3b thrL terminator + ptlpAr BCD8 mf-Lon ssrA</td>
 
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</tr>
 
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</table>
 
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</center>
 
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Revision as of 02:52, 18 October 2018

Parts

Parts

Besides our 3G parts, library we are submitting a series of heat inducible reporters(mScarlet) and protease(mf-Lon) which we are using to construct IFFLs with different parameters.
3. Heat inducible IFFLs
Name
Part ID
K2680064 J23107 TPL + ptlpA B0034m mScarlet-I pdt-3d thrL terminator + ptlpAr BCD8 mf-Lon ssrA
K2680069 J23107 TPL + ptlpA B0034m mScarlet-I thrL terminator + ptlpAr BCD8 mf-Lon ssrA
K2680063 J23107 TPL + ptlpA B0034m mScarlet-I pdt-3b thrL terminator + ptlpAr BCD8 mf-Lon ssrA

1. Heat inducible protease

Name
Part ID
K2680050 J23107 ts-CI + R0051 mf-Lon
K2680057 J23100 ts-CI + R0051 mf-Lon ssrA
K2680058 J23107 TPL + ptlpA BCD8 mf-lon thrL
K2680059 J23107 TPL + ptlpA BCD8 mf-lon ssrA thrL
K2680060 J23107 TPL + ptlpAr BCD8 mf-lon thrL
K2680061 J23107 TPL + ptlpAr BCD8 mf-lon ssrA thrL

2. Heat inducible reporters

Name
Part ID
K2680051 J23107 ts-CI + R0051 mScarlet-I pdt#3
K2680052 J23107 ts-CI + R0051 mScarlet-I pdt#3b
K2680053 J23100 ts-CI + R0051 mScarlet-I pdt#3
K2680054 J23100 ts-CI + R0051 mScarlet-I pdt#3b
K2680062 J23107 TPL + ptlpA b0034m mScarlet-I pdt#3
K2680065 J23107 TPL + ptplAr B0034 mScarlet-I pdt#3
K2680066 J23107 TPL + ptplA B0034 mScarlet-I pdt#3b
K2680067 J23107 TPL + ptplAr B0034 mScarlet-I pdt#3b
K2680068 J23107 ts-CI + R0051 mScarlet-I
K2680070 J23107 ts-CI + R0051 mScarlet-I pdt#3e
K2680071 J23107 TPL + ptlpA b0034m mScarlet-I pdt#3e