Difference between revisions of "Team:Bielefeld-CeBiTec/Parts"

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         <tr>
 
         <tr>
 
             <th>BBa_K2638991</th>
 
             <th>BBa_K2638991</th>
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             <td></td>  
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             <td>I0500, BBa_K2638992</td>  
 
             <td>araBAD and RBS and Mutated Human Ferritin Heavy Chain (without Stop)</td>
 
             <td>araBAD and RBS and Mutated Human Ferritin Heavy Chain (without Stop)</td>
 
             <td>Vanessa Krämer</td>
 
             <td>Vanessa Krämer</td>
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             <td>1210</td>
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             <td>1765</td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
 
             <th>BBa_K2638997</th>
 
             <th>BBa_K2638997</th>
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             <td></td>  
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             <td>BBa_K118902, BBa_B0030, BBa_I0500</td>  
 
             <td>araBAD and RBS and Human Ferritin Heavy Chain</td>
 
             <td>araBAD and RBS and Human Ferritin Heavy Chain</td>
 
             <td>Vanessa Krämer</td>
 
             <td>Vanessa Krämer</td>
−
             <td>552</td>
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             <td>1793</td>
 
         </tr>
 
         </tr>
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</table>
 
</table>

Revision as of 04:59, 4 December 2018

Parts
The iGEM parts registry includes more than 20,000 BioBricks. The standardized form of BioBricks are the basis so all present and future iGEM teams can build on existing BioBricks to achieve their projects. This year we integrated more than 50 existing BioBricks into our project and we submitted more than 80 BioBricks. Further iGEM teams can realize their project by building on our BioBricks.
All of our submitted basic parts are listed in Table 1.
We are very proud of our favorite basic part BBa_K2638002, an impressive copper importer.
The best composite of our team BBa_K2638703 is a second generation targed vector for siRNA silencing. All other composite parts are listed in table 2.
Our part improvement is a modified human ferritin BBa_K2638999. The human ferritin was submitted by iGEM Calgary in 2013 and is an iron storage protein. We changed certain amino acid residues to add the ability to form silver and gold nanoparticles.
During our project we had to deal with gene expression, which requires a certain promoter strength. To enable a comparison of different promoter strengths, we developed a measurement vector. Furthermore, we characterized the system with our promoter-RBS library. The vector and the library are submitted as best part collection. The BioBricks are: BBa_K2638560 and BBa_K2638400 - BBa_K2638426.

Table 1:All BioBricks submitted by iGEM Bielefeld CeBiTec 2018.
Identifier Type Description Designer Length
BBa_K2638000 Coding Heavy Metal Transporter (hmtA) Erika Schneider 1920
BBa_K2638001 Coding CopC coding sequence Erika Schneider 387
BBa_K2638002 Coding CopD CDS Erika Schneider 912
BBa_K2638100 Coding alkyl hydroperoxide reductase, C22 subunit (ahpC) Johannes Ruhnau 564
BBa_K2638103 Coding glutathione synthetase (gshB) Johannes Ruhnau 951
BBa_K2638105 Coding catalase HPI (katG) Johannes Ruhnau 2181
BBa_K2638106 Coding superoxide dismutase (sodA) Johannes Ruhnau 621
BBa_K2638120 Coding glutathione peroxidase (btuE) Johannes Ruhnau 552
BBa_K2638121 Coding glutathione reductase (gor) Johannes Ruhnau 1353
BBa_K2638150 Coding Glutathione gamma-glutamylcysteinyltransferase 1 Matthias Otto 1458
BBa_K2638151 Coding Glutathione gamma-glutamylcysteinyltransferase 1 with RBS and pTet promoter Matthias Otto 1535
BBa_K2638152 Coding Glutathione gamma-glutamylcysteinyltransferase 1 with RBS and Anderson promoter Matthias Otto 1519
BBa_K2638153 Coding Glutathione gamma-glutamylcysteinyltransferase 1 with AraBAD promoter and RBS Matthias Otto 2694
BBa_K2638200 Coding OprC (TonB dependent copper transport porin) Jakob Zubek 2127
BBa_K2638426 Reporter mRFP+ Levin Joe Klages 827
BBa_K2638601 Coding Human Ferritin Heavy Chain Copper Enhanced Binding Christoph Geske 552
BBa_K2638602 Coding Human Ferritin Heavy Chain E-Helix Removed Weaker Binding Christoph Geske 480
BBa_K2638603 Coding Human Ferritin Heavy Chain Copper Enhanced Binding and E-Helix removed Christoph Geske 474
BBa_K2638701 Generator Seamless siRNA Expression Vektor pGuide Antonin Lenzen 1780
BBa_K2638702 Generator Seamless siRNA Expression Vektor for Hfq binding siRNAs Antonin Lenzen 1865
BBa_K2638707 Reporter siRNA Target Vector with BFP and a GGGGS Linker Antonin Lenzen 2779
BBa_K2638708 Reporter siRNA Target Vector with BFP and an EAAAK Linker Antonin Lenzen 2779
BBa_K2638709 Reporter siRNA Target Vector with BFP and a cMyc Linker Antonin Lenzen 2779
BBa_K2638710 Reporter siRNA Target Vector with BFP and XP Linker Antonin Lenzen 2779
BBa_K2638711 Reporter siRNA Target Vector with AmilCP and a GGGGS Linker Antonin Lenzen 1226
BBa_K2638712 Reporter siRNA Target Vector with AmilCP and EAAAK Linker Antonin Lenzen 1226
BBa_K2638713 Reporter siRNA Target Vector with AmilCP and a cMyc Linker Antonin Lenzen 1226
BBa_K2638714 Reporter siRNA Target Vector with AmilCP and XP Linker Antonin Lenzen 1226
BBa_K2638751 Regulatory Synthetic Origin of Replication B Antonin Lenzen 837
BBa_K2638752 Regulatory pLac including a TetO sequence in place of the CAP binding sequence Antonin Lenzen 206
BBa_K2638753 Regulatory pLac including a Tet operator sequence behind the Lac operator Antonin Lenzen 213
BBa_K2638754 Coding E. coli Bacterioferritin Bfr Antonin Lenzen 477
BBa_K2638755 Coding Ferredoxin reductase Antonin Lenzen 747
BBa_K2638756 Coding Bacterial non-heme ferritin FtnA from Escherichia coli Antonin Lenzen 498
BBa_K2638757 Coding Ferric Uptake Regulator (Fur) from Escherichia coli K12 Antonin Lenzen 447
BBa_K2638758 Generator Seamless siRNA Expression Vektor for Omp-Utr protected siRNAs Antonin Lenzen 1897
BBa_K2638759 Generator Seamless siRNA Expression Vektor with an Hfq binding tag and OmpA 5'-UTR Antonin Lenzen 1982
BBa_K2638998 Coding araBAD and RBS and Mutated Human Ferritin Heavy Chain Vanessa Krämer 1566
BBa_K2638999 Coding Mutated Human Ferritin Heavy Chain Vanessa Krämer 552
Table 2:All Composite Parts submitted by iGEM Bielefeld CeBiTec 2018.
Identifier Components Description Designer Length
BBa_K2638003 BBa_K525998, BBa_K2638001 T7 + RBS + CopC Erika Schneider 425
BBa_K2638004 BBa_K525998, BBa_K2638002 T7 + RBS + CopD Erika Schneider 950
BBa_K2638005 BBa_I0500, BBa_B0030, BBa_K2638001 T7 + RBS + CopC Erika Schneider 1626
BBa_K2638006 BBa_I0500, BBa_B0030, BBa_K2638002 T7 + RBS + CopD Erika Schneider 2151
BBa_K2638109 BBa_R0040, BBa_K1460002 PTetR + CRS5 Johannes Ruhnau 1032
BBa_K2638110 BBa_J61101, BBa_K2638103, BBa_J61101, BBa_K2638150 PTetR + gshA + gshB + Phyto Johannes Ruhnau 2450
BBa_K2638112 BBa_I0500, BBa_B0030, BBa_B0034, BBa_K2638121, BBa_B0034, BBa_K2638103, BBa_B0034, BBa_K2638120 PTetR + gshA + gshB + GSR Johannes Ruhnau 4167
BBa_K2638113 BBa_I0500, BBa_B0030, BBa_B0034, BBa_K2638100 PTetR + ahpC + ahpF Johannes Ruhnau 1823
BBa_K2638114 BBa_R0040, BBa_K554003, BBa_K1104200 PTetR + SoxR + RBS + OxyR Johannes Ruhnau 1489
BBa_K2638117 BBa_R0040, BBa_J61101, BBa_K2638106 PTetR + RBS + sodA + KatE Johannes Ruhnau 701
BBa_K2638118 BBa_R0040, BBa_B0034, BBa_K2638406, BBa_B0034, BBa_K2638105 PTetR + RBS + sodA + KatG Johannes Ruhnau 2908
BBa_K2638201 BBa_K525998, BBa_K2638200 OprC (TonB dependent copper transport porin, BBa_K2638200) with T7 promotor and RBS (BBa_K525998) Jakob Zubek 2165
BBa_K2638204 BBa_I0500, BBa_B0030, BBa_K2638200 OprC (TonB dependent copper transport porin, BBa_K2638200) with AraC/pBad Jakob Zubek 3366
BBa_K2638400 BBa_K2638500 Combination of BBa_K2638500 + BBa_K2638560 Levin Joe Klages 889
BBa_K2638401 BBa_K2638502, BBa_K2638426 13 Levin Joe Klages 889
BBa_K2638402 BBa_K2638503, BBa_K2638426 14 Levin Joe Klages 889
BBa_K2638403 BBa_K2638504, BBa_K2638426 15 Levin Joe Klages 889
BBa_K2638404 BBa_K2638506, BBa_K2638426 17 Levin Joe Klages 889
BBa_K2638405 BBa_K2638507, BBa_K2638426 18 Levin Joe Klages 889
BBa_K2638406 BBa_K2638509, BBa_K2638426 110 Levin Joe Klages 889
BBa_K2638407 BBa_K2638510, BBa_K2638426 111 Levin Joe Klages 889
BBa_K2638408 BBa_K2638511, BBa_K2638426 112 Levin Joe Klages 889
BBa_K2638409 BBa_K2638517, BBa_K2638426 118 Levin Joe Klages 903
BBa_K2638410 BBa_K2638520, BBa_K2638426 21 Levin Joe Klages 890
BBa_K2638411 BBa_K26358522, BBa_K2638426 23 Levin Joe Klages 890
BBa_K2638412 BBa_K2638525, BBa_K2638426 26 Levin Joe Klages 890
BBa_K2638413 BBa_K2638526, BBa_K2638426 27 Levin Joe Klages 890
BBa_K2638414 BBa_K2638528, BBa_K2638426 29 Levin Joe Klages 890
BBa_K2638415 BBa_K2638531, BBa_K2638426 212 Levin Joe Klages 890
BBa_K2638416 BBa_K2638532, BBa_K2638426 213 Levin Joe Klages 889
BBa_K2638417 BBa_K2638534, BBa_K2638426 215 Levin Joe Klages 900
BBa_K2638418 BBa_K2638537, BBa_K2638426 218 Levin Joe Klages 904
BBa_K2638419 BBa_K2638542, BBa_K2638426 33 Levin Joe Klages 889
BBa_K2638420 BBa_K2638545, BBa_K2638426 36 Levin Joe Klages 889
BBa_K2638421 BBa_K2638548, BBa_K2638426 39 Levin Joe Klages 889
BBa_K2638422 BBa_K2638551, BBa2638426 312 Levin Joe Klages 889
BBa_K2638423 BBa_K2638554, BBa_K2638426 315 Levin Joe Klages 899
BBa_K2638424 BBa_K26358556, BBa_2638426 317 Levin Joe Klages 909
BBa_K2638425 BBa_K2638557, BBa_K2638426 318 Levin Joe Klages 903
BBa_K2638703 BBa_K2638716, BBa_B0010, BBa_B0012, BBa_R0010, BBa_B0032, BBa_K592100 siRNA Target Vector 2 with BFP and GGGGS Linker Antonin Lenzen 2787
BBa_K2638704 BBa_K2638717, BBa_B0010, BBa_B0012, BBa_R0010, BBa_B0032, BBa_K592100 siRNA Target Vector 2 with BFP and GGGGS Linker Antonin Lenzen 2787
BBa_K2638705 BBa_K2638718, BBa_B0010, BBa_B0012, BBa_R0010, BBa_B0032, BBa_K592100 siRNA Target Vector 2 with BFP and cMyc Linker Antonin Lenzen 2787
BBa_K2638706 BBa_K2638719, BBa_B0010, BBa_B0012, BBa_R0010, BBa_B0032, BBa_K592100 siRNA Target Vector 2 with BFP and XP Linker Antonin Lenzen 2787
BBa_K2638991 I0500, BBa_K2638992 araBAD and RBS and Mutated Human Ferritin Heavy Chain (without Stop) Vanessa Krämer 1765
BBa_K2638997 BBa_K118902, BBa_B0030, BBa_I0500 araBAD and RBS and Human Ferritin Heavy Chain Vanessa Krämer 1793