Difference between revisions of "Team:Toronto/InterLab"
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<ul> | <ul> | ||
<li>Purpose of this calibration: To transform absorbance data to a OD<sub>600</sub> measurement, calculate a plate-reader specific (Tecan Infinite 200 Pro) conversion factor for OD<sub>600</sub> from Abs<sub>600</sub> calculated for Ludox CL-X on a mass spectrophotometer.</li> | <li>Purpose of this calibration: To transform absorbance data to a OD<sub>600</sub> measurement, calculate a plate-reader specific (Tecan Infinite 200 Pro) conversion factor for OD<sub>600</sub> from Abs<sub>600</sub> calculated for Ludox CL-X on a mass spectrophotometer.</li> | ||
| − | <li></li | + | <li>Beer-Lambert’s law of absorbance dictates that optical path length plays a fundamental role in determining absorbance: </li> |
| − | + | ||
| − | + | ||
| − | + | ||
<img src="https://static.igem.org/mediawiki/2018/b/b5/T--Toronto--7_25_2018_Interlab_lambert-law.gif" alt="lambert-law"> | <img src="https://static.igem.org/mediawiki/2018/b/b5/T--Toronto--7_25_2018_Interlab_lambert-law.gif" alt="lambert-law"> | ||
| + | <li>This is necessary because cuvettes (used in photometer) have a fixed path optical path length when using light scattering to measure absorbance as opposed to the varying path lengths of wells in a 96-well plate which change as the volume of sample added in them changes.</li> | ||
| + | <li>Results: Cell density readings can thus be converted to OD600 by multiplying correction factor value, <b>4.138</b>.</li> | ||
| + | </ul> | ||
| + | |||
<img src="https://static.igem.org/mediawiki/2018/b/b7/T--Toronto--7_25_2018_Interlab_Capture.png" alt="Capture"> | <img src="https://static.igem.org/mediawiki/2018/b/b7/T--Toronto--7_25_2018_Interlab_Capture.png" alt="Capture"> | ||
<img src="https://static.igem.org/mediawiki/2018/3/37/T--Toronto--7_25_2018_Interlab_Capture1.png" alt="Capture1"> | <img src="https://static.igem.org/mediawiki/2018/3/37/T--Toronto--7_25_2018_Interlab_Capture1.png" alt="Capture1"> | ||
Revision as of 00:39, 26 July 2018
- Purpose of this calibration: To transform absorbance data to a OD600 measurement, calculate a plate-reader specific (Tecan Infinite 200 Pro) conversion factor for OD600 from Abs600 calculated for Ludox CL-X on a mass spectrophotometer.
- Beer-Lambert’s law of absorbance dictates that optical path length plays a fundamental role in determining absorbance:
- This is necessary because cuvettes (used in photometer) have a fixed path optical path length when using light scattering to measure absorbance as opposed to the varying path lengths of wells in a 96-well plate which change as the volume of sample added in them changes.
- Results: Cell density readings can thus be converted to OD600 by multiplying correction factor value, 4.138.
