Difference between revisions of "Team:Bielefeld-CeBiTec/Accumulation"

Revision as of 16:08, 29 July 2018

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Copper Accumulation System

Overview

One important goal of nanoFactory is to create an efficient accumulation system for heavy metal ions, in this case of copper, in order to subsequently form nanoparticles in Escherichia coli. The main component for such a system is a highly efficient uptake system. Since copper is toxic to all types of cells (Ladomersky & Petris, 2015), the presence of transporters for the active and selective uptake of copper ions may initially come as a surprise. But copper also carries out important functions in many cells. As a cofactor for many different enzymes such as superoxide dismutase, it is primarily involved in electron transfer and dioxygen transport and activation (Solomon et al., 2014). Accordingly, there must be natural absorption mechanisms for this trace element into both the periplasm and the cytoplasm. Nevertheless non-bonded, dissolved copper in particular is very toxic, so there are also efficient export systems in E. coli to get rid of excess copper (Rensing & Grass, 2003). In order to create an efficient accumulation system for copper ions, efficient and selective uptake systems must be expressed on the one hand and existing export systems must be suppressed on the other.

Import

Export

Firstname	Lastname	Age
Jill	Smith	50
Eve	Jackson	94
John	Doe	80

References

hier Referenz eins
hier Referenz zwei
.........

@@ Line 48: / Line 48: @@
                     <h2>Overview</h2>
                     <br><br>
+                   <article>
 One important goal of nanoFactory is to create an efficient accumulation system for heavy metal                         ions, in this case of copper, in order to subsequently form nanoparticles in <i>Escherichia coli</i>. The main component for such a system is a highly efficient uptake system.
 Since copper is toxic to all types of cells (Ladomersky & Petris, 2015), the presence of transporters for the active and selective uptake of copper ions may initially come as a surprise. But copper also carries out important functions in many cells. As a cofactor for many different enzymes such as superoxide dismutase, it is primarily involved in electron transfer and dioxygen transport and activation (Solomon <i>et al.</i>, 2014). Accordingly, there must be natural absorption mechanisms for this trace element into both the periplasm and the cytoplasm. Nevertheless non-bonded, dissolved copper in particular is very toxic, so there are also efficient export systems in <i>E. coli</i> to get rid of excess copper (Rensing & Grass, 2003). In order to create an efficient accumulation system for copper ions, efficient and selective uptake systems must be expressed on the one hand and existing export systems must be suppressed on the other.
+                   </article>
                     <h2>Import</h2>