Difference between revisions of "Team:Bielefeld-CeBiTec/Ferritin"

Revision as of 14:44, 29 August 2018

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Iron Uptake by Ferritin

Fe(III) and Fe(II) are important cofactors used in the active sites of a broad range of enzymes. Therefore, the iron homeostasis is a process tightly controlled in all organisms. Metal ions can also have toxic side effects when the amount of free ions inside the cell is too high because copper and iron ions can catalyse the formation of reactive oxygen species (ROS) which are highly toxic to the cell. For this reason we measured the toxicity of different ion concentrations and searched for countermeasures (see Toxicity).
To prohibit toxic side effects the efflux and influx of ions are tightly controlled by a set of regulators, importers, exporters and storage proteins. Iron ions are stored in ferritin and ferritin-like proteins. Bacteria like Escherichia coli have ferritin (FtnA) as well as bacterioferritin proteins (BfrB). These proteins can mineralize iron by oxidizing soluble Fe(II)-ions to Fe(III)-ions which form oxides and become insoluble. The Ferric uptake regulator (Fur) controls the expression of ferritins and several proteins used in iron mineralisation and mobilisation. In the presence of sufficient iron, Fur is activated by binding Fe(II)-ions and blocks further expression of iron acquisition proteins. In a low-iron environment the ions get desorbed and Fur is inactivated allowing expression of iron uptake-proteins. While Fur negatively regulates expression, an siRNA (ryhB), which is also negatively regulated by Fur, is used as a second level of regulation. The siRNA is transcribed in absence of Fur and regulates the expression of iron storage proteins. So in a high-iron environment Fur is active and ryhB is inactive so iron-storage proteins like FtnA can be expressed. In a low-iron environment, the expression of iron-storage proteins is stopped. Instead, the absence of Fur allows the cell to produce proteins needed for iron mobilisation, namely the Ferredoxin reductase (Fpr) and the bacterioferritin-associated Ferredoxin (Bfd). It was shown that Fpr, Bfd and nicotinamide adenine dinucleotide phosphate (NADPH) are needed to mobilize Fe(II) from BfrB, while only Fpr and NADPH are needed for the iron-mobilisation from FtnA (see figure 1).
Figure 1: Mechanism of Fe(II) mobilisation from BfrB (A) and from FtnA (B). To mobilise Fe(II)-ions from BfrB, Fpr, Bfd and NADPH are needed. To mobilise Fe(II)-ions from FtnA only NADPH and Fpr are needed. Figure from Rivera (2017).
FtnA is a homo 24mer with an iron-oxidase center and a mineralisation center at each monomer. BfrB coordinates a heme-iron complex between two subunits, leading to 12 heme complexes in each BfrB protein complex.
Ferritins are used in vitro for the production of different nanoparticles composed of AgS, CuS or AuS. Another use is the isolation of phosphate from water.(Jacobs et al., (2010))
Additionally mobilising great amounts of iron ions at the same time could potentially be used to kill cells.

Iwahori, K., Takagi, R., Kishimoto, N., & Yamashita, I. (2011). A size controlled synthesis of CuS nano-particles in the protein cage, apoferritin. Materials Letters, 65(21-22), 3245-3247.
Jacobs, J. F., Hasan, M. N., Paik, K. H., Hagen, W. R., & van Loosdrecht, M. (2010). Development of a bionanotechnological phosphate removal system with thermostable ferritin. Biotechnology and bioengineering, 105(5), 918-923.
Massé, E., & Gottesman, S. (2002). A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli. Proceedings of the National Academy of Sciences, 99(7), 4620-4625.
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REFERENZEN

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-Fe(III) and Fe(II) are important cofactors used in the active sites of a broad range of enzymes. Therefore, the iron homeostasis is a process tightly controlled in all organisms. Metal ions can also have toxic side effects when the amount of free ions inside the cell is too high because copper and iron ions can catalyse the formation of reactive oxygen species (ROS) which are highly toxic to the cell. For this reason we measured the toxicity of different ion concentrations and searched for countermeasures (see <a href src="https://2018.igem.org/Team:Bielefeld-CeBiTec/Toxicity_Theory"> Toxicity</A>. </br>
+Fe(III) and Fe(II) are important cofactors used in the active sites of a broad range of enzymes. Therefore, the iron homeostasis is a process tightly controlled in all organisms. Metal ions can also have toxic side effects when the amount of free ions inside the cell is too high because copper and iron ions can catalyse the formation of reactive oxygen species (ROS) which are highly toxic to the cell. For this reason we measured the toxicity of different ion concentrations and searched for countermeasures (see <a href src="https://2018.igem.org/Team:Bielefeld-CeBiTec/Toxicity_Theory"> Toxicity</A>). </br>
 To prohibit toxic side effects the efflux and influx of ions are tightly  controlled by a set of regulators, importers, exporters and storage proteins.
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