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               Optimization of the Arg1 construct for flotation in E. coli should yield the function of efficient, inducible flotation in transformed E. coli cells. The Arg1 operon is greater than 7kb and the secondary Gvp genes have no known fundamental role in the production of the gas vesicles. We aim to reduce the size of the Arg1 operon to exclude the secondary proteins, thereby relying on the primary proteins, and test flotation in E. coli cells. In addition to laboratory experimentation for characterization of the Arg1 construct, gas vesicles expressed by it, and the function/necessity of the gas vesicle proteins that go into forming the vesicles, our team will be doing extensive mathematical modelling and computational analysis of data to model growth dynamics of the engineered cell strain and the kinetics of metal binding surface proteins, as well as to determine the buoyant force imparted by gas vesicle expression. These secondary models will be fed into a larger model that aims to predict the potential performance and operating characteristics for our proposed cellular bioremediation platform on an industrial scale.<br><br>

               Optimization of the Arg1 construct for flotation in E. coli should yield the function of efficient, inducible flotation in transformed E. coli cells. The Arg1 operon is greater than 7kb and the secondary Gvp genes have no known fundamental role in the production of the gas vesicles. We aim to reduce the size of the Arg1 operon to exclude the secondary proteins, thereby relying on the primary proteins, and test flotation in E. coli cells. In addition to laboratory experimentation for characterization of the Arg1 construct, gas vesicles expressed by it, and the function/necessity of the gas vesicle proteins that go into forming the vesicles, our team will be doing extensive mathematical modelling and computational analysis of data to model growth dynamics of the engineered cell strain and the kinetics of metal binding surface proteins, as well as to determine the buoyant force imparted by gas vesicle expression. These secondary models will be fed into a larger model that aims to predict the potential performance and operating characteristics for our proposed cellular bioremediation platform on an industrial scale.<br><br>

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