Difference between revisions of "Team:Linkoping Sweden/Parts"

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<table>
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  <tr>
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    <th>Name</th>
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    <th>Marker</th>
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    <th>Class</th>
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    <th>Promoter</th>
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    <th>Insert</th>
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  </tr>
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  <tr>
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    <td>pGroE7</td>
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    <td>Chloramphenicol</td>
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    <td>B</td>
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    <td>pBAD</td>
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    <td>GroE</td>
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  </tr>
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  <tr>
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    <td>pG-KJE8</td>
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    <td>Chloramphenicol</td>
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    <td>B</td>
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    <td>pBAD</td>
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    <td>GroE, DnaKJE</td>
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  </tr>
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  <tr>
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    <td>pG-Tf2</td>
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    <td>Chloramphenicol</td>
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    <td>B</td>
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    <td>pBAD</td> 
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    <td>GroE, Tf</td>
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  </tr>
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  <tr>
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    <td>pEGFP-Tau</td>
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    <td>Kanamycin</td>
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    <td>A</td>
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    <td>T7</td>
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    <td>EGFP-Tau</td>
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  </tr>
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  <tr>
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    <td>pEGFP-Aβ</td>
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    <td>Kanamycin</td>
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    <td>A</td>
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    <td>T7</td>
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    <td>EGFP-Aβ</td>
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  </tr>
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  <tr>
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    <td>pSB4A5-GroES</td>
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    <td>Ampicillin</td>
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    <td>C</td>
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    <td>pTet</td>
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    <td>GroES</td>
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  </tr>
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</table>
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Revision as of 15:56, 19 September 2018

LiU iGEM

Parts

All parts

Collection

This is a collection of all parts we worked with, for more information about the parts, their function and results, please visit the results page or the parts site on parts.igem.org.

  • Composite parts: BBa_K2671420
  • Basic Parts: BBa_K2671337
  • Improved parts:
  • Verified collaboration parts: K2602011, K2602014, K2602016, K2602021, K2602024, K2602026
  • Verified biobrick: BBa_I746909

Primers used for all our experiments can be found below.

  • Vector reverse (VR): attaccgcctttgagtgagc
  • Vector forward 2 (VF2): tgccacctgacgtctaagaa
  • Backbone SNP reverse (BSNPR): ctctagaagcggccgcgaat
  • Backbone ENX forward (BENXF): tactagtagcggccgctg
  • Liu iGEM17 site directed mutagenesis reverse (SDMR): cccgaaccgctttacttg
  • Liu iGEM17 site directed mutagenesis forward (SDMR): ctcaggaagcactagtggctcggac
  • Liu iGEM17 mNG forward (mNG-F): ggactggtgcaggtcgaaga
Name Marker Class Promoter Insert
pGroE7 Chloramphenicol B pBAD GroE
pG-KJE8 Chloramphenicol B pBAD GroE, DnaKJE
pG-Tf2 Chloramphenicol B pBAD GroE, Tf
pEGFP-Tau Kanamycin A T7 EGFP-Tau
pEGFP-Aβ Kanamycin A T7 EGFP-Aβ
pSB4A5-GroES Ampicillin C pTet GroES