Difference between revisions of "Team:Rice/Parts"

(Designed table for parts)
Line 1: Line 1:
 
{{Rice}}
 
{{Rice}}
 +
 
<html>
 
<html>
 +
<head>
 +
    <link href="https://fonts.googleapis.com/css?family=Assistant:800|Didact+Gothic|Open+Sans:300" rel="stylesheet">
 +
</head>
 
<style>
 
<style>
−
table{
+
    body{
−
    border-collapse: collapse;
+
background-color: white !important;
−
}
+
}
−
th, td {
+
table{
−
    border-bottom: 1px solid #ddd;
+
        position: absolute !important;
−
}
+
width: 72%;
−
tr:hover {background-color:#f5f5f5;}
+
  margin: auto;
−
th {
+
  float: right;
−
    background-color: #82C7A5;
+
  padding-top: 10px;
−
     color: white;
+
  padding-right: 10px;
−
}
+
        margin-right: 3%
 +
z-index:-1000 !important;
 +
}
 +
th{
 +
color:#1097bc!important;
 +
font-family: 'Didact Gothic', sans-serif!important;
 +
font-size: 26px!important;
 +
                        line-height: 120%;
 +
                        text-align: left;
 +
}
 +
    tr{
 +
        color:#000000!important;
 +
font-family: 'Open Sans', sans-serif!important;
 +
font-size: 18px!important;
 +
}
 +
    p{
 +
    margin: 2%;
 +
    color:#000000!important;
 +
font-family: 'Open Sans', sans-serif!important;
 +
font-size: 18px!important;  
 +
     }
 +
.container{
 +
  width: 72%!important;
 +
  margin: auto!important;
 +
  float: right!important;
 +
  padding-top: 10px!important;
 +
  padding-right: 10px!important;
 +
        margin-right: 3%!important;
 +
z-index: -500!important;
 +
    }
 
</style>
 
</style>
 +
<body>
 +
<div class="container">
 +
<table align="center" class="container" style="background:white">
 +
<tr>
 +
    <th>Part #</th>
 +
    <th>Name</th>
 +
    <th>Type</th>
 +
    <th>Description</th>
 +
  </tr>
 +
  <tr>
 +
    <td><a href='http://parts.igem.org/Part:BBa_K2194000' style='color:rgb(153,117,185)'><font color='Indigo  '>BBa_K2194000</font></a></td>
 +
    <td>chrR6 Chromate Reductase Enzyme</td>
 +
    <td>basic</td>
 +
    <td width = "50%"><img src="https://static.igem.org/mediawiki/parts/4/40/ChrR6_yellow.svg" style="height:70%; width:20%;">
 +
<p> The chrR6 protein is a mutated/enhanced version (<sup>Tyr</sup>128<sup>Asn</sup>) of the chrR enzyme originally found in <i>Escherichia coli</i>. It has chromate and uranyl reductase activity. BioBrick BBa_K2194000 contains only the cds for this protein.
  
−
<div class="column full_size">
+
<p>
−
<h1>Parts</h1>
+
</td>
−
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
  </tr>
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
  <tr>
−
</div>
+
    <td><a href='http://parts.igem.org/Part:BBa_K2194002' style='color:rgb(153,117,185)'><font color='Indigo  '>BBa_K2194002</font></a></td>
−
 
+
    <td width = "20%"><i>PgntK</i> Negative Feedback Membrane Stress Promoter </td>
−
<div class="column full_size">
+
    <td>basic</td>
−
<div class="highlight decoration_background">
+
    <td><img src="https://static.igem.org/mediawiki/parts/d/d0/Rice_PgntK_without_RBS.svg" style="height:70%; width:20%;" > <p> The <i>PgntK</i> promoter is a native <i>Escherichia coli</i> negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the <i>gntK</i> gene in <i>Escherichia coli</i>, which has been previously experimentally shown to incorporate the full <i>Pgntk</i> promoter region.</p> </td>
−
<h3>Note</h3>
+
  </tr>
−
<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
+
  <tr>
−
</div>
+
    <td><a href='http://parts.igem.org/Part:BBa_K2194001' style='color:rgb(153,117,185)'><font color='Indigo  '>BBa_K2194001</font></a></td>
−
</div>
+
    <td>Sulfate Binding Protein (sbp) cds</td>
−
 
+
    <td>basic</td>
−
<div class="clear extra_space"></div>
+
    <td><img src="https://static.igem.org/mediawiki/parts/5/57/Rice_sbp.svg" style="height:30%; width:20%;"> <p> The sbp protein is found in<i>Escherichia coli</i> and specifically binds sulfate and related species (like chromate and selenate) for their transmembrane transport via the ABC transporter system encoded by the <i>cysPUWA</i> genes. BioBrick BBa_K2194001 contains only the cds for this protein.
−
<div class="line_divider"></div>
+
</p></td>
−
<div class="clear extra_space"></div>
+
  </tr>
−
 
+
  <tr>
−
 
+
  <td><a href='http://parts.igem.org/Part:BBa_K2194003' style='color:rgb(153,117,185)'><font color='Indigo  '>BBa_K2194003</font></a></td>
−
 
+
  <td>cysPUWA Sulfate Transport System cds</td>
−
 
+
  <td>composite</td>
−
 
+
  <td><img src="https://static.igem.org/mediawiki/parts/d/d4/CysPUWA_rice.svg" style="width:70% !important; height:90%" !important; > <p>The <i>cysPUWA</i> genes encode the sulfate ABC membrane transporter in <i>Escherichia coli</i>. This system also transports related species such as chromate. BioBrick BBa_K2194003 contains the cds for <i>cysP</i>, <i>cysU</i>, <i>cysW</i>, and <i>cysA</i> in the context in which they appear in <i>E. coli</i> MG1655.
−
<div class="column two_thirds_size">
+
</p></td>
−
<div class="highlight decoration_B_full">
+
  </tr>
−
 
+
  <tr>
−
<h3>Adding parts to the registry</h3>
+
  <td><a href='http://parts.igem.org/Part:BBa_K2194004' style='color:rgb(153,117,185)'><font color='Indigo  '>BBa_K2194004</font></a></td>
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
  <td>Sulfate Transport System w/ Negative Feedback for Membrane Stress</td>
−
 
+
  <td>composite</td>
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
+
  <td><img src="https://static.igem.org/mediawiki/parts/1/11/PgntK%2C_sbp%2C_cys.svg" style="height:100%; width:90%;" > <p>This transcriptional unit codes for the genetic parts necessary to bind and transport chromate, sulfate, and related species across the cell membrane. BioBrick BBa_K2194004 is a composite part composed of negative feedback membrane stress promoter <i>PgntK</i> (<a href="http://parts.igem.org/Part:BBa_K2194002">BBa_K2194002</a>), a synthetic RBS, cysPUWA proteins (<a href="http://parts.igem.org/Part:BBa_K2194003">BBa_K2194003</a>), Elowitz RBS (<a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>), sulfate binding protein sbp (<a href="http://parts.igem.org/Part:BBa_K2194001">BBa_K2194001</a>), and a synthetic terminator.  </td>
−
<div class="button_link">
+
  </tr>
−
<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
+
  <tr>
−
ADD PARTS
+
  <td><a href='http://parts.igem.org/Part:BBa_K2194005' style='color:rgb(153,117,185)'><font color='Indigo  '>BBa_K2194005</font></a></td>
−
</a>
+
  <td> chrR6 Chromate Reductase Enzyme with Chromate-sensitive Promoter </td>
−
</div>
+
  <td>composite</td>
−
 
+
  <td><img src="https://static.igem.org/mediawiki/parts/f/f9/Rice_Pchr%2BChrR6.svg" style="height:100%; width:40%;"> <p>This transcriptional unit codes for a chromate reductase enzyme under a chromate-sensitive promoter. BioBrick BBa_K2194005 is a composite part composed of <i>Pchr</i> chromate-sensitive promoter ("cr-promoter" feature of <a href="http://parts.igem.org/Part:BBa_K1758312">BBa_K1758312</a>), with rbs (<a href="http://parts.igem.org/Part:BBa_K1758100">BBa_K1758100</a>), and chrR6 chromate reductase (<a href="http://parts.igem.org/Part:BBa_K2194000">BBa_K2194000</a>).  </p></td>
−
</div>
+
  </tr>
−
</div>
+
</table>
−
 
+
</div>
−
 
+
</body>
−
 
+
−
<div class="column third_size">
+
−
<div class="highlight decoration_A_full">
+
−
<h3>Inspiration</h3>
+
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
−
 
+
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
+
−
<ul>
+
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
+
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
+
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
 
+
−
 
+
−
<div class="clear extra_space"></div>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
<div class="column full_size">
+
−
 
+
−
<h3>What information do I need to start putting my parts on the Registry?</h3>
+
−
<p>The information needed to initially create a part on the Registry is:</p>
+
−
<ul>
+
−
<li>Part Name</li>
+
−
<li>Part type</li>
+
−
<li>Creator</li>
+
−
<li>Sequence</li>
+
−
<li>Short Description (60 characters on what the DNA does)</li>
+
−
<li>Long Description (Longer description of what the DNA does)</li>
+
−
<li>Design considerations</li>
+
−
</ul>
+
−
 
+
−
<p>
+
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
+
−
 
+
−
</div>
+
−
 
+
−
 
+
−
<div class="clear extra_space"></div>
+
−
<div class="line_divider"></div>
+
−
<div class="clear extra_space"></div>
+
−
 
+
−
<div class="column full_size">
+
−
<h3>Part Table </h3>
+
−
 
+
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
−
 
+
−
</html>
+
−
<groupparts>iGEM18 Rice</groupparts>
+
−
<html>
+
−
</div>
+
−
 
+
−
<table style="width:100%">
+
−
  <tr>
+
−
    <th>Part name</th>
+
−
    <th>Type</th>
+
−
    <th> Description </th>
+
−
    <th> Link to part </th>
+
−
  </tr>
+
−
  <tr align = "center">
+
−
    <td>Sample </td>
+
−
    <td> N/A </td>
+
−
    <td> Just a sample </td>
+
−
    <td> <a> sample link </a> </td>
+
−
  </tr>
+
−
  <tr align = "center">
+
−
    <td>Sample </td>
+
−
    <td> N/A </td>
+
−
    <td> Just a sample </td>
+
−
    <td> <a> sample link </a> </td>
+
−
  </tr>
+
−
</table>
+
−
 
+
−
 
+
 
</html>
 
</html>

Revision as of 01:52, 2 October 2018

Part # Name Type Description
BBa_K2194000 chrR6 Chromate Reductase Enzyme basic

The chrR6 protein is a mutated/enhanced version (Tyr128Asn) of the chrR enzyme originally found in Escherichia coli. It has chromate and uranyl reductase activity. BioBrick BBa_K2194000 contains only the cds for this protein.

BBa_K2194002 PgntK Negative Feedback Membrane Stress Promoter basic

The PgntK promoter is a native Escherichia coli negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the gntK gene in Escherichia coli, which has been previously experimentally shown to incorporate the full Pgntk promoter region.
BBa_K2194001 Sulfate Binding Protein (sbp) cds basic

The sbp protein is found inEscherichia coli and specifically binds sulfate and related species (like chromate and selenate) for their transmembrane transport via the ABC transporter system encoded by the cysPUWA genes. BioBrick BBa_K2194001 contains only the cds for this protein.

BBa_K2194003 cysPUWA Sulfate Transport System cds composite

The cysPUWA genes encode the sulfate ABC membrane transporter in Escherichia coli. This system also transports related species such as chromate. BioBrick BBa_K2194003 contains the cds for cysP, cysU, cysW, and cysA in the context in which they appear in E. coli MG1655.

BBa_K2194004 Sulfate Transport System w/ Negative Feedback for Membrane Stress composite

This transcriptional unit codes for the genetic parts necessary to bind and transport chromate, sulfate, and related species across the cell membrane. BioBrick BBa_K2194004 is a composite part composed of negative feedback membrane stress promoter PgntK (BBa_K2194002), a synthetic RBS, cysPUWA proteins (BBa_K2194003), Elowitz RBS (BBa_B0034), sulfate binding protein sbp (BBa_K2194001), and a synthetic terminator.

BBa_K2194005 chrR6 Chromate Reductase Enzyme with Chromate-sensitive Promoter composite

This transcriptional unit codes for a chromate reductase enzyme under a chromate-sensitive promoter. BioBrick BBa_K2194005 is a composite part composed of Pchr chromate-sensitive promoter ("cr-promoter" feature of BBa_K1758312), with rbs (BBa_K1758100), and chrR6 chromate reductase (BBa_K2194000).