Difference between revisions of "Team:NUDT CHINA/Notebook"

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  <caption>Fig.1 OD600 reference point</caption>
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</td>
 
</td>
 
<td width="538">
 
<td width="538">
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<p>Finish the Phosphorylation and annealing of the oligos&nbsp;with Qijie Xu</p>
+
<p>Finish the culture of A549 cell and optimization of culture condition&nbsp;with Chuanyang Liu</p>
 
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<td width="538">
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<p>Work on miRNA locker assembly with Qijie Xu</p>
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<p>Work on Western Blot assay for target gene expression&nbsp;with Chuanyang Liu</p>
 
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</td>
 
<td width="538">
 
<td width="538">
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<p>Finish the Asymmetric PCR to generate ssDNAs from dsDNAs&nbsp;with Qijie Xu</p>
+
<p>Do Western Blot assay EMT markers&nbsp;with Chuanyang Liu</p>
 
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</td>
 
</tr>
 
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</td>
 
</td>
 
<td width="538">
 
<td width="538">
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<p>Construct parts&nbsp;with Qijie Xu</p>
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<p>Finish the Cell proliferation and migration assay of miR-214 Locker group&nbsp;with Chuanyang Liu</p>
 
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</td>
 
</tr>
 
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</td>
 
</td>
 
<td width="538">
 
<td width="538">
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<p>Work on the experimental validation of parts&nbsp;with Qijie Xu</p>
+
<p>Finish the Cell proliferation and migration assay of miR-654 Locker group&nbsp;with Chuanyang Liu</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
 
</table>
 
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</center>
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</div>
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<table class="table table-striped">
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<div class="col-md-push-1 col-md-10">
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  <caption>Fig.1 OD600 reference point</caption>
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<center>
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<table class="table table-striped">
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  <caption>scx</caption>
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<tbody>
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<tr>
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<p><strong>D</strong><strong>ata</strong></p>
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<td width="538">
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<p><strong>Project</strong></p>
 +
</td>
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</tr>
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<tr>
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<td width="153">
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<p><strong>6.15-7.4</strong></p>
 +
</td>
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<td width="538">
 +
<p>Finish the culture of A549 cell and optimization of culture condition&nbsp;with Chuanyang Liu</p>
 +
</td>
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</tr>
 +
<tr>
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<td width="153">
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<p><strong>7.5-</strong><strong>8.13</strong></p>
 +
</td>
 +
<td width="538">
 +
<p>Work on Western Blot assay for target gene expression&nbsp;with Chuanyang Liu</p>
 +
</td>
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</tr>
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<tr>
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<td width="153">
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<p><strong>8.</strong><strong>14-8.26</strong></p>
 +
</td>
 +
<td width="538">
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<p>Do Western Blot assay EMT markers&nbsp;with Chuanyang Liu</p>
 +
</td>
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</tr>
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<tr>
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<td width="153">
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<p><strong>8.27-9.22</strong></p>
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</td>
 +
<td width="538">
 +
<p>Finish the Cell proliferation and migration assay of miR-214 Locker group&nbsp;with Chuanyang Liu</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="153">
 +
<p><strong>9.22-10.14</strong></p>
 +
</td>
 +
<td width="538">
 +
<p>Finish the Cell proliferation and migration assay of miR-654 Locker group&nbsp;with Chuanyang Liu</p>
 +
</td>
 +
</tr>
 +
</tbody>
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</table>
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</center> 
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</div>
  
 +
<div class="col-md-push-1 col-md-10">
 
<center>
 
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</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Finish the Phosphorylation and annealing of the oligos&nbsp;with Chushu Zhu</p>
+
<p>Finish the culture of A549 cell and optimization of culture condition&nbsp;with Chuanyang Liu</p>
 
</td>
 
</td>
 
</tr>
 
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</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Work on miRNA locker assembly with Chushu Zhu</p>
+
<p>Work on Western Blot assay for target gene expression&nbsp;with Chuanyang Liu</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
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</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Finish the Asymmetric PCR to generate ssDNAs from dsDNAs&nbsp;with Chushu Zhu</p>
+
<p>Do Western Blot assay EMT markers&nbsp;with Chuanyang Liu</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
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</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Construct parts&nbsp;with Chushu Zhu</p>
+
<p>Finish the Cell proliferation and migration assay of miR-214 Locker group&nbsp;with Chuanyang Liu</p>
 
</td>
 
</td>
 
</tr>
 
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</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Work on the experimental validation of parts&nbsp;with Chushu Zhu</p>
+
<p>Finish the Cell proliferation and migration assay of miR-654 Locker group&nbsp;with Chuanyang Liu</p>
 
</td>
 
</td>
 
</tr>
 
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Revision as of 16:25, 7 October 2018

Development of A Novel

Blood-MicroRNA Handy Detection System with CRISPR

Chuanyang Liu, Changsong Gao, Chushu Zhu and Qijie Xu completed most of the experiments.

scx

Data

Project

6.15-7.4

Finish the culture of A549 cell and optimization of culture condition with Chuanyang Liu

7.5-8.13

Work on Western Blot assay for target gene expression with Chuanyang Liu

8.14-8.26

Do Western Blot assay EMT markers with Chuanyang Liu

8.27-9.22

Finish the Cell proliferation and migration assay of miR-214 Locker group with Chuanyang Liu

9.22-10.14

Finish the Cell proliferation and migration assay of miR-654 Locker group with Chuanyang Liu
scx

Data

Project

6.15-7.4

Finish the culture of A549 cell and optimization of culture condition with Chuanyang Liu

7.5-8.13

Work on Western Blot assay for target gene expression with Chuanyang Liu

8.14-8.26

Do Western Blot assay EMT markers with Chuanyang Liu

8.27-9.22

Finish the Cell proliferation and migration assay of miR-214 Locker group with Chuanyang Liu

9.22-10.14

Finish the Cell proliferation and migration assay of miR-654 Locker group with Chuanyang Liu
scx

Data

Project

6.15-7.4

Finish the culture of A549 cell and optimization of culture condition with Chuanyang Liu

7.5-8.13

Work on Western Blot assay for target gene expression with Chuanyang Liu

8.14-8.26

Do Western Blot assay EMT markers with Chuanyang Liu

8.27-9.22

Finish the Cell proliferation and migration assay of miR-214 Locker group with Chuanyang Liu

9.22-10.14

Finish the Cell proliferation and migration assay of miR-654 Locker group with Chuanyang Liu
scx

Data

Project

6.15-7.4

Finish the culture of A549 cell and optimization of culture condition with Chuanyang Liu

7.5-8.13

Work on Western Blot assay for target gene expression with Chuanyang Liu

8.14-8.26

Do Western Blot assay EMT markers with Chuanyang Liu

8.27-9.22

Finish the Cell proliferation and migration assay of miR-214 Locker group with Chuanyang Liu

9.22-10.14

Finish the Cell proliferation and migration assay of miR-654 Locker group with Chuanyang Liu