Difference between revisions of "Team:NUDT CHINA/Notebook"

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<div class="col-md-12 column" alt="cover_photo" style="width:100%;height:250px;background-size:cover;background-position:0px -350px; background-image:url(https://static.igem.org/mediawiki/2016/thumb/3/3f/NUDT_CHINA2016_FILES-img-banner-3.0.png/800px-NUDT_CHINA2016_FILES-img-banner-3.0.png);background-repeat:no-repeat;">
 
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  <p style="font-size:36px;margin: 0 0%;padding: 8% 0 0 10%;color: white;">Designed Protein Degradation Method Based on</p>
 
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<p style="font-size:36px;margin: 0 0%;padding: 0 0 0 10%;color: white;">Trim21 And Nanobody</p>
 
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                                       <h1 style="font-size: 40px;font-weight: 1200"></h1>

Revision as of 14:57, 12 October 2018

Jiaxin Ma ,Tianyi Zhang,Chenyu Lu and Chenxing Sun completed most of the experiments.

Jiaxin Ma

Data

Project

6.15-7.3

Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Tianyi Zhang.

7.4-8.3

Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Tianyi Zhang.

8.4-8.15

Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Tianyi Zhang.

8.16-8.28

Work on repeated validation of GFP interfering efficiency with Tianyi Zhang.

8.29-9.22

Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Tianyi Zhang.

9.22-10.10

Work on the proliferation and migration assay of cells with ErbB3 interfered with Tianyi Zhang.
Tianyi Zhang

Data

Project

6.15-7.3

Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Jiaxin Ma.

7.4-8.3

Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Jiaxin Ma.

8.4-8.15

Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Jiaxin Ma.

8.16-8.28

Work on repeated validation of GFP interfering efficiency with Jiaxin Ma.

8.29-9.22

Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Jiaxin Ma.

9.22-10.10

Work on the proliferation and migration assay of cells with ErbB3 interfered with Jiaxin Ma.
Chenyu Lu

Data

Project

6.15-7.3

Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenxing Sun.

7.4-8.3

Hela Cell culture and preparatory works on fluorescence detection with Chenxing Sun.

8.4-8.28

Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenxing Sun.

8.29-9.22

Construct Parts with Chenxing Sun.

9.22-10.10

Work on the experimental validation of parts with Chenxing Sun.
Chenxing Sun

Data

Project

6.15-7.3

Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenyu Lu .

7.4-8.3

Hela Cell culture and preparatory works on fluorescence detection with Chenyu Lu .

8.4-8.28

Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenyu Lu .

8.29-9.22

Construct Parts with Chenyu Lu .

9.22-10.10

Work on the experimental validation of parts with Chenyu Lu .