Difference between revisions of "Team:NYU Abu Dhabi/Parts"
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<img src="https://static.igem.org/mediawiki/2018/9/9d/T--NYU_Abu_Dhabi--Parts1.png"width="300" class="center"> | <img src="https://static.igem.org/mediawiki/2018/9/9d/T--NYU_Abu_Dhabi--Parts1.png"width="300" class="center"> | ||
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| − | <h2><i>Figure | + | <h2><i>Figure: Agarose gel electrophoresis showing PCR amplification of: gbpA BioBrick <a href="http://parts.igem.org/Part:BBa_K2808000"target="_blank">BBA_K2808000</a>; hipO <a href="http://parts.igem.org/Part:BBa_K2808001"target="_blank">BBA_K2808001</a>; invA <a href="http://parts.igem.org/Part:BBa_K2808002"target="_blank">BBA_K2808002</a>; lmo0733 <a href="http://parts.igem.org/Part:BBa_K2808003"target="_blank">BBA_K2808003</a>. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) negative control for gbpA BioBrick; (Lane 4) hipO BioBrick; (Lane 5) negative control for hipO BioBrick; (Lane 6) invA BioBrick; (Lane 7) negative control for invA BioBrick; (Lane 8) lmo0733 BioBrick; (Lane 9) negative control for lmo0733 BioBrick. Lanes 2, 4, 6, and 8 show bands around 1019 bp, 203 bp, 818 bp, and 403 bp respectively as expected for the PCR products of the gbpA, hipO, invA, and lmo0733 BioBricks respectively. |
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<img src="https://static.igem.org/mediawiki/2018/1/1b/T--NYU_Abu_Dhabi--Parts2.png"width="300" class="center"> | <img src="https://static.igem.org/mediawiki/2018/1/1b/T--NYU_Abu_Dhabi--Parts2.png"width="300" class="center"> | ||
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| − | <h2><i>Figure | + | <h2><i>Figure: Agarose gel electrophoresis showing double digestion of the BioBricks: gbpA <a href="http://parts.igem.org/Part:BBa_K2808000"target="_blank">BBA_K2808000</a>; hipO <a href="http://parts.igem.org/Part:BBa_K2808001"target="_blank">BBA_K2808001</a>; invA <a href="http://parts.igem.org/Part:BBa_K2808002"target="_blank">BBA_K2808002</a>; lmo0733 <a href="http://parts.igem.org/Part:BBa_K2808003"target="_blank">BBA_K2808003</a> with EcoRI and PstI. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) gbpA BioBrick digested with EcoRI and PstI. (Lane 4) hipO BioBrick; (Lane 5) hipO BioBrick digested with EcoRI and PstI; (Lane 6) invA BioBrick; (Lane 7) invA BioBrick digested with EcoRI and PstI; (Lane 8) lmo0733 BioBrick; (Lane 9) lmo0733 BioBrick digested with EcoRI and PstI. The lengths of the pSB1C3 backbone, gbpA gene, hipO gene, invA gene, and lmo0733 genes are 2070 bp, 1458 bp, 1152 bp, 2065 bp, and 510 bp respectively. |
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<img src="https://static.igem.org/mediawiki/2018/b/bf/T--NYU_Abu_Dhabi--Parts3.png" height="400" class="center2"> | <img src="https://static.igem.org/mediawiki/2018/b/bf/T--NYU_Abu_Dhabi--Parts3.png" height="400" class="center2"> | ||
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| − | <h2><i>Figure | + | <h2><i>Figure: Agarose gel electrophoresis showing double digestion of the gbpA BioBrick <a href="http://parts.igem.org/Part:BBa_K2808000"target="_blank">BBA_K2808000</a> (gbpA gene (1458 bp) inserted into the pSB1C3 backbone (2070 bp)) with EcoRI and PstI. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) gbpA BioBrick digested with EcoRI and PstI. |
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Revision as of 05:16, 13 October 2018
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Parts
| Name | Type | Description |
|---|---|---|
| BBa_K2808000 | DNA | N-acetyl glucosamine-binding protein A (gbpA) gene for the detection of Vibrio cholera The gbpA gene in Vibrio cholerae codes for the N-acetyl glucosamine-binding protein A (GbpA), which is a chitin-binding protein involved in the attachment of V. cholerae to environmental chitin surfaces and human intestinal cells. The gbpA gene has been found to be consistently present and highly conserved in V. cholerae. GbpA has been used as a target gene for the species-specific detection of V. cholerae O1, O139, Non-O1/Non-O139. |
| BBa_K2808001 | DNA | N-benzoylglycine amidohydrolase (hippuricase) (hipO) gene for the detection of Campylobacter jejuni hipO gene codes for N-benzoylglycine amidohydrolase (hippuricase) in Campylobacter jejuni. Due its specificity to the organism and the lack of toxicity, various research projects have used this gene for the detection of C. jejuni through techniques such as PCR. |
| BBa_K2808002 | DNA | Invasion protein (invA) gene for the detection of Salmonella typhimurium The invA gene is involved in the invasion of the cells of the intestinal epithelium and could be involved in the translocation of the InvE protein in Salmonella typhimurium. Due its specificity to the organism and the lack of toxicity, various research projects have used this gene for the detection of S. typhimurium. |
| BBa_K2808003 | DNA | lmo0773 alcohol dehydrogenase gene for the detection of Listeria monocytogenes The lmo0773 alcohol dehydrogenase is involved in oxidoreductase activity. Due its specificity to the organism and the lack of toxicity, various research projects have used this gene for the detection of Listeria monocytogenes. |
| BBa_K2808004 | Primer | gbpA F3 2 |
| BBa_K2808005 | Primer | gbpA B3 1 |
| BBa_K2808006 | Primer | Hipo PCR forward 1 |
| BBa_K2808007 | Primer | Hipo PCR reverse 1 |
| BBa_K2808008 | Primer | Hipo PCR forward 2 |
| BBa_K2808009 | Primer | Hipo PCR reverse 2 |
| BBa_K2808010 | Primer | PCR forward Salmonella 1 |
| BBa_K2808010 | Primer | PCR forward Salmonella 1 |
| BBa_K2808011 | Primer | PCR reverse Salmonella 2 |
| BBa_K2808012 | Primer | PCR forward Listeria |
| BBa_K2808013 | Primer | lmo0733 B3 2 |
| BBa_K2808014 | Primer | gbpA F3 1 |
| BBa_K2808015 | Primer | gbpA B3 1 |
| BBa_K2808016 | Primer | gbpA FIP 1 |
| BBa_K2808017 | Primer | gbpA BIP 1 |
| BBa_K2808018 | Primer | hipO F3 |
| BBa_K2808019 | Primer | hipO B3 |
| BBa_K2808020 | Primer | hipO FIP |
| BBa_K2808021 | DNA | hipO BIP |
| BBa_K2808022 | Primer | invA F3 1 |
| BBa_K2808023 | Primer | invA B3 1 |
| BBa_K2808024 | Primer | invA FIP 1 |
| BBa_K2808025 | Primer | invA BIP 1 |
| BBa_K2808026 | Primer | lmo0733 F3 2 |
| BBa_K2808027 | Primer | lmo0733 B3 2 |
| BBa_K2808028 | Primer | lmo0733 FIP 2 |
| BBa_K2808029 | Primer | lmo0733 BIP 2 |
| BBa_K2808030 | Primer | gbpA RPA F2 |
| BBa_K2808031 | Primer | gbpA RPA R2 | BBa_K2808032 | Primer | invA RPA F1 | BBa_K2808033 | Primer | invA RPA R1 | BBa_K2808034 | Primer | lmo0733 RPA F1 | BBa_K2808035 | Primer | lmo0733 RPA R1 |
BioBricks PCR amplification
Figure: Agarose gel electrophoresis showing PCR amplification of: gbpA BioBrick BBA_K2808000; hipO BBA_K2808001; invA BBA_K2808002; lmo0733 BBA_K2808003. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) negative control for gbpA BioBrick; (Lane 4) hipO BioBrick; (Lane 5) negative control for hipO BioBrick; (Lane 6) invA BioBrick; (Lane 7) negative control for invA BioBrick; (Lane 8) lmo0733 BioBrick; (Lane 9) negative control for lmo0733 BioBrick. Lanes 2, 4, 6, and 8 show bands around 1019 bp, 203 bp, 818 bp, and 403 bp respectively as expected for the PCR products of the gbpA, hipO, invA, and lmo0733 BioBricks respectively.
BioBricks double digestion reaction
Figure: Agarose gel electrophoresis showing double digestion of the BioBricks: gbpA BBA_K2808000; hipO BBA_K2808001; invA BBA_K2808002; lmo0733 BBA_K2808003 with EcoRI and PstI. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) gbpA BioBrick digested with EcoRI and PstI. (Lane 4) hipO BioBrick; (Lane 5) hipO BioBrick digested with EcoRI and PstI; (Lane 6) invA BioBrick; (Lane 7) invA BioBrick digested with EcoRI and PstI; (Lane 8) lmo0733 BioBrick; (Lane 9) lmo0733 BioBrick digested with EcoRI and PstI. The lengths of the pSB1C3 backbone, gbpA gene, hipO gene, invA gene, and lmo0733 genes are 2070 bp, 1458 bp, 1152 bp, 2065 bp, and 510 bp respectively.
gbpA BioBrick double digestion reaction
