Difference between revisions of "Team:TUST China/Attributions"

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    <title>attibution</title>
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            <h1 style="top: 160px;text-align: center">Detection of Tetracycline</h1>
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        <h1 style="top: 160px;text-align: center;font-size: 3vw;">Attributions</h1>
 +
        <div class="head-content"> <p>We would like to thank all of those who have supported and assisted us in the laboratory, in the school and in society since the
 +
            beginning of our tetracycline detecting and degradation project. Without those help, this project wouldn't have been possible and cannot been progressing well.</p>
 +
 
 +
    </div>
 +
    </div>
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</div>
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<div class="container">
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 +
    <div class="container content-cen">
 +
 
 +
 
 +
        <h2>General Support</h2>
 +
        <p>We would like to extend our sincerest thanks to our team Primary PI, <strong>Professor Cheng Zhong</strong>. His enthusiasm for the synthesis biology and assistance for the lab resources could not be more greatly appreciated.
 +
            We would like to thank the <strong>PhD student LongHui Huang</strong>, our instructors. He selfless help us in the molecular experiments operation and help us improve our project design to better achieve our final anticiptions.
 +
            We also want to thank our advisors,<strong> TingYue Deng</strong>. She gave lots of advices when we found my team members and built our new iGEM team. What’s more, she also gave us suggestions when we perfected our project parts, including the human practice and parts submission.
 +
        </p>
 +
        <h2>Project & Work Support</h2>
 +
        <p>We would like to thank each team members of 2018 TUST_China iGEM team. And this the team member list and their respective attributions in the below.</p>
 +
        <h2>Team Members</h2>
 +
      <center> <table>
 +
            <tbody style="line-height: 40px;">
 +
            <tr>
 +
                <td><li>WenXin Lin</li></td>
 +
                <td><li>ZhenYu Xiong</li></td>
 +
 
 +
            </tr>
 +
            <tr>
 +
                <td><li>HaoRan Xu</li></td>
 +
                <td><li>YuWen Zhang</li></td>
 +
 
 +
            </tr>
 +
            <tr>
 +
                <td><li>ZeYun You</li></td>
 +
                <td><li>YuXuan Quan</li></td>
 +
 
 +
            </tr>
 +
            <tr>
 +
                <td><li>LeLing Xu</li></td>
 +
                <td><li>XiangYu Zhou</li></td>
 +
 
 +
            </tr>
 +
            <tr>
 +
                <td><li>FangYuan Zhao</li></td>
 +
                <td><li>Meng Wang</li></td>
 +
 
 +
            </tr>
 +
            <tr><td><li>XiaoJin Li</li></td>
 +
                <td><li>Ge Gao</li></td></tr>
 +
            <tr><td><li>Kai Lu</li></td><td><li>Jing Li</li></td></tr>
 +
            </tbody>
 +
        </table></center>
 +
        <h2>Distribution</h2>
 +
<div style="display:block;width: 100%;height: 800px;"><center>
 +
        <div class="qcontainer">
 +
            <div class="film">
 +
                <div class="face front"><div class="round-none"><p>Wiki</p></div></div>
 +
                <div class="face back"><span><br>
 +
 
 +
<br>
 +
YuXuan Quan<br>
 +
</span></div>
 +
            </div>
 +
        </div>
 +
        <div class="qcontainer">
 +
            <div class="film">
 +
                <div class="face front"><div class="round-none"><p>Chasis</p></div></div>
 +
                <div class="face back"><span>WenXin Lin<br>
 +
LeLing Xu<br>
 +
YuWen Zhang<br>
 +
Meng Wang<br>
 +
Jing Li<br>
 +
</span></div>
 +
            </div>
 
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         </div>
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         <div class="qcontainer">
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            <div class="film">
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                <div class="face front"><div class="round-none"><p>Verification</p></div></div>
 +
                <div class="face back"><span>WenXin Lin<br>
 +
XiangYu Zhou<br>
 +
ZeYun You<br>
 +
LeLing Xu<br>
 +
Kai Lu<br>
  
−
            <h2>Sample Pretreatment</h2>
+
</span></div>
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            <p >
+
             </div>
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                Aquaculture, fish pond water samples and stool samples. (From the area with high tetracycline content. Water samples need to be protected from light, and the following operations are required to avoid light.)
+
        </div>
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            </p>
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        <div class="qcontainer">
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             <h2>Pretreatment of Water Samples:</h2>
+
             <div class="film">
−
            <p>1. Centrifuge the water sample in the 50ml centrifuge tube（8000-10000r/min, 5min）and take the supernatant in the clean centrifuge tube. Repeat many times until there is no visible impurity. (Repeat this procedure no less than three times.)</p>
+
                 <div class="face front"><div class="round-none"><p>H&P</p></div></div>
−
            <p>2. The water sample in the centrifugal tube is passed through the membrane and packed into 10 clean centrifugal tubes, each containing 10 ml.</p>
+
                <div class="face back"><span>WenXin Lin<br>
−
             <p>3. Placing 10 centrifuge tubes at -80 degrees Celsius for no less than 24 hours.</p>
+
ZeYun You<br>
−
            <p>4. The samples were sealed with preservative film after 24 hours of freezing, and several small holes were punctured. The samples were lyophilized by freeze-drying machine until they were all turned into powder.</p>
+
FangYuan Zhao<br>
−
            <h2>Pretreatment of Feces</h2>
+
ZhenYu Xiong<br>
−
            <p>1. Take appropriate amount of fecal samples in 50 ml centrifugal tube with water solution, centrifugal(8000-10000r/min，5min）, take supernatant in a clean centrifugal tube. Adding water to dissolve, centrifugation
+
Kai Lu<br>
−
                 （8000-10000r/min，5min）, and take the supernatant in centrifugation. (Repeated operation above, all the supernatant was placed in centrifuge
+
−
                tube.
+
−
            </p>
+
−
            <p>2. The supernatant will be centrifugally repeated several times until there is no visible impurity.</p>
+
−
            <p>3. The samples in the centrifugal tube were passed through the mmbrane and packed into 10 clean centrifugal tubes, each containing 10 ml.</p>
+
−
            <p>4. Placing 10 centrifuge tubes at -80 degrees Celsius for less than 24 hours.</p>
+
−
            <p>5. Freezing the sample after 24 hours with a plastic wrap and seal a plurality of small holes.</p>
+
−
            <p>6. The lyophilized machine was used for freeze-drying operations until all the samples were changed into powder.</p>
+
−
            <h2>Subsequent Operations After Freeze Drying</h2>
+
−
            <p>1. The freeze-dried powder is merged into a centrifugal tube, dissolved in a small amount of water (just dissolved, water cannot be excessive), and put in - 80 degrees Celsius freezing 24 hours, freeze-dried.</p>
+
−
            <p>2. Adding 1mlDMF to dissolve the powder (over film treatment) and get the final samples. (Need to operate in hood)</p>
+
−
            <h2>Detection: High Performance Liquid Chromatography</h2>
+
−
            <p>1. Instrument: WatersC18ODS-3 column (250 mm 4.6 mm, 5 m).</p>
+
−
            <p>2. Reagents:</p>
+
−
            <p>Mobile phase A solution: methanol (filtration treatment) B liquid: ddH2O</p>
+
−
            <p>Note:</p>
+
−
            <p>Ultrasound is needed for at least half an hour before use and the temperature should not be too high.
+
−
                Injection needles need to be cleaned by mobile phase. Use brown liquid vial
+
−
            </p>
+
−
            <p>3. Setting speed: 1 mL/minColumn <p>temperature: 35 degrees Celsius</p> <p>Absorption peak: 355nm</p>
+
  
−
            <p>Injection volume: 20ul</p>
+
</span></div>
−
             </p>
+
             </div>
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            <p>Setting Concentration Gradient：</p>
+
        </div>
−
             <table class="table table-striped" >
+
        <div class="qcontainer">
−
                 <thead>
+
             <div class="film">
−
                    <tr>
+
                 <div class="face front"><div class="round-none"><p>HPLC</p></div></div>
−
                        <td>Time</td>
+
                 <div class="face back"><span><br>HaoRan Xu<br>
−
                        <td>A</td>
+
XiaoJin Li<br>
−
                        <td>B</td>
+
Ge Gao<br>
−
                    </tr>
+
−
                 </thead>
+
−
                <tbody>
+
−
                <tr>
+
−
                    <td style="border-right: solid 2px #add9c0;">0-2min</td>
+
−
                    <td>5%</td>
+
−
                    <td>95%</td>
+
  
−
                </tr>
+
</span></div>
−
                <tr>
+
            </div>
−
                    <td style="border-right: solid 2px #add9c0;">2.1min</td>
+
        </div>
−
                    <td>40%</td>
+
        <div class="qcontainer">
−
                    <td>60%</td>
+
            <div class="film">
−
                </tr>
+
                <div class="face front"><div class="round-none"><p>Modeling</p></div></div>
−
                <tr>
+
                <div class="face back"><span><br>LeLing Xu<br>
−
                    <td style="border-right: solid 2px #add9c0;">8min</td>
+
Ge Gao<br>
−
                    <td>70%</td>
+
−
                    <td>30%</td>
+
  
−
                </tr>
+
</span></div>
−
                <tr>
+
            </div>
−
                    <td>8.1-12min</td>
+
        </div>
−
                    <td>90%</td>
+
        <div class="qcontainer">
−
                    <td>10%</td>
+
            <div class="film">
 +
                <div class="face front"><div class="round-none"><p>Art</p></div></div>
 +
                <div class="face back"><span><br>Ying Ma<br>
 +
Jing Li<br>
  
 +
</span></div>
 +
            </div>
 +
        </div>
 +
        <div class="qcontainer">
 +
            <div class="film">
 +
                <div class="face front"><div class="round-none"><p >Logistics</p></div></div>
 +
                <div class="face back"><span><br>ZeYun You<br>
 +
ZhenYu Xiong<br>
 +
HaoRan Xu<br>
  
−
                </tr>
 
−
                <tr>
 
−
                    <td>12-15min</td>
 
−
                    <td>5％</td>
 
−
                    <td>95%</td>
 
  
 +
</span></div>
 +
            </div>
 +
        </div>
 +
        <div class="qcontainer">
 +
            <div class="film">
 +
                <div class="face front"><div class="round-none"><p>Pre</p></div></div>
 +
                <div class="face back"><span>YuWen Zhang<br>
 +
WenXin Lin<br>
 +
FangYuan Zhao<br>
 +
Ge Gao<br>
  
−
                </tr>
 
  
−
                </tbody>
+
 
−
            </table>
+
</span></div>
−
            <p>This method should peak at about 6.72min.</p>
+
             </div>
−
             <p class="lead">Please keep all the data and images of the liquid phase and send the results to TUST_China</p>
+
−
            <p><i class="footiconfont bigicon">&#xe609;&nbsp;&nbsp;tustigem2018@163.com</i></p>
+
 
         </div>
 
         </div>
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    </div>
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 +
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 +
        </center>
 +
 +
        <h2>Funding Support</h2>
 +
        <p>First and foremost, we would like to thank <strong>Professor Cheng Zhong</strong> again for providing us with vital financial and lab resource support. We would also like to thank the following organizations and offices for generously providing us with the financial support necessary for conducting and completing our project in this year:</p>
 +
        <p><strong>Tianjin University of Science & Technology Academic Registrar
 +
            College of Biotechnology, Tianjin University of Science & Technology
 +
            Shandong BenZhen Cosmetics CO. LTD.
 +
        </strong></p>
 +
        <a href="https://mp.weixin.qq.com/s/ybDigKkiJZC4uPmwLUYoAg" style="margin-left: 100px;"><img src="images/spologo.png" width="100px"></a>
 +
        <a href="http://en.tust.edu.cn/default.html " style="margin-left: 100px;"><img src="https://static.igem.org/mediawiki/2018/4/4c/T--TUST_China--tustlogo.png" width="300px"></a>
 
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{{{{TUST_China/foot}}

Revision as of 07:32, 14 October 2018

nav

protocol

Attributions

We would like to thank all of those who have supported and assisted us in the laboratory, in the school and in society since the beginning of our tetracycline detecting and degradation project. Without those help, this project wouldn't have been possible and cannot been progressing well.

General Support

We would like to extend our sincerest thanks to our team Primary PI, Professor Cheng Zhong. His enthusiasm for the synthesis biology and assistance for the lab resources could not be more greatly appreciated. We would like to thank the PhD student LongHui Huang, our instructors. He selfless help us in the molecular experiments operation and help us improve our project design to better achieve our final anticiptions. We also want to thank our advisors, TingYue Deng. She gave lots of advices when we found my team members and built our new iGEM team. What’s more, she also gave us suggestions when we perfected our project parts, including the human practice and parts submission.

Project & Work Support

We would like to thank each team members of 2018 TUST_China iGEM team. And this the team member list and their respective attributions in the below.

Team Members

  • WenXin Lin
    		•
  • ZhenYu Xiong
    		•
  • HaoRan Xu
    		•
  • YuWen Zhang
    		•
  • ZeYun You
    		•
  • YuXuan Quan
    		•
  • LeLing Xu
    		•
  • XiangYu Zhou
    		•
  • FangYuan Zhao
    		•
  • Meng Wang
    		•
  • XiaoJin Li
    		•
  • Ge Gao
  • Kai Lu
  • Jing Li
    •

    Distribution

    Wiki



    YuXuan Quan

    Chasis

    WenXin Lin
    LeLing Xu
    YuWen Zhang
    Meng Wang
    Jing Li

    Verification

    WenXin Lin
    XiangYu Zhou
    ZeYun You
    LeLing Xu
    Kai Lu

    H&P

    WenXin Lin
    ZeYun You
    FangYuan Zhao
    ZhenYu Xiong
    Kai Lu

    HPLC


    HaoRan Xu
    XiaoJin Li
    Ge Gao

    Modeling


    LeLing Xu
    Ge Gao

    Art


    Ying Ma
    Jing Li

    Logistics


    ZeYun You
    ZhenYu Xiong
    HaoRan Xu

    Pre

    YuWen Zhang
    WenXin Lin
    FangYuan Zhao
    Ge Gao

    Funding Support

    First and foremost, we would like to thank Professor Cheng Zhong again for providing us with vital financial and lab resource support. We would also like to thank the following organizations and offices for generously providing us with the financial support necessary for conducting and completing our project in this year:

    Tianjin University of Science & Technology Academic Registrar College of Biotechnology, Tianjin University of Science & Technology Shandong BenZhen Cosmetics CO. LTD.

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