Results from One Test Tube Electroporations

At the core of the Broad Host Range Kit is the One Test Tube Method of testing plasmids in a host bacteria. This is how the majority of people who get our kit for the first time will begin their experiments. The idea here is that a researcher will be able to transform multiple assembled plasmids at once ,and then through selective plating see which works in the non-model organisms. This will give the researcher insight for what origin of replication works, which is the first step in building a plasmid to genetically engineering an organism. Results will be confirmed by one of two ways: assessing the phenotype produced by the reporter gene or sequencing at the barcode region . The reporter is either a fluorescent protein or chromoprotein specific and each color designates an origin. At a genomic level, a unique, non-coding sequence of DNA does the same thing, specifying which origin it is paired with. We created our one tube mixture of the initial assemblies by calculating an equimolar concentration.