Difference between revisions of "Team:Linkoping Sweden/Demonstrate"
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<img src="https://static.igem.org/mediawiki/parts/9/92/T--Linkoping_Sweden--expdesign.png"/> | <img src="https://static.igem.org/mediawiki/parts/9/92/T--Linkoping_Sweden--expdesign.png"/> | ||
| − | <h4>Figure x. A simple explaination on how we archived our experimental results. The plasmids shown is the same as before, where A can be either of mNG-Aß1-42, EGFP-Aß1-42, a-synuclein-EGFP and Tau0N4R-EGFP. The concentrations used for inducing the chaperone plasmids was 0.25 mg/ml L-arabinose, 200 ng/ml tetracycline. For the substrate plasmids we used 0.5 mM IPTG, and the substrate was induced 30 mins after the chaperone plasmids. | + | <h4>Figure x. A simple explaination on how we archived our experimental results. The plasmids shown is the same as before, where A can be either of mNG-Aß1-42, EGFP-Aß1-42, a-synuclein-EGFP and Tau0N4R-EGFP, B is always pGroE7 and C is pSB4A5-GroES. The concentrations used for inducing the chaperone plasmids was 0.25 mg/ml L-arabinose, 200 ng/ml tetracycline. For the substrate plasmids we used 0.5 mM IPTG, and the substrate was induced 30 mins after the chaperone plasmids. |
<h3> | <h3> | ||
Results for the mNG-Aß1-42 substrate protein | Results for the mNG-Aß1-42 substrate protein | ||
Revision as of 17:00, 14 October 2018
Demonstrate
All work done on BBa_K2671420
Plasmids used during the characterization of our biobrick
Overview of our experimental design to archive the results shown below
