Then we put forward two questions: Why phase separation in cells can produce membraneless organelles? And how can we design our system to fulfill its intended functions? Like oil in water, the contents of cells can separate into droplets. According to physical principles, the process where material self-assemble into organelles is described as ‘phase separation’, which is the conversion of a single-phase system into a multiphase system. In general, materials flow to regions with low chemical potential instead of low concentration. Finally, the components no longer distribute uniformly but form granules locally which are organelles in the cell. That is to say, the main work to synthesize an organelle is to fulfill phase separation in a cell. Then, how can we do it? Composition can switch rapidly through changes in scaffold concentration or multivalency. And our design was inspired by recent works showing that multivalency drives protein phase separation and formation of synthetic organelles. What’s more, we take our inspiration from existing life systems and previous works. For example, Intrinsic Disordered Regions are the symbol of massive phase separation in the cell. They interact with each other through the van der Waals force, hydrophobic effect and electrostatic attraction. And there are many interactions like this in nature, such as FKBP and FRB, SUMO and SIM, SH3 and PRM, phyB and PIF6. Thus, we can make good use of them to induce our designed organelles and regulate them variously.! In a conclusion，multivalency drives protein’s -self-assemblyies and interaction binds the parts together. It means, interaction can induce phase separation and multivalency can make larger assemblies, which are two essential elementmodules in our design and ensure the formation of synthetic organelles.

To design multivalent modules, it is not ideal to use multiple repeat domains, which not only will make the protein extremely large and bring difficulties to DNA recombination, but also may be problematic for making transgenic animals. Thus, instead of using multiple repeats, we turned to de novo-designed homo-oligomeric coiled coils. And we named these coiled coils as HO-Tag (homo-oligomeric tag). They are short peptides, ~30 amino acids, therefore they are ideal tags to introduce multivalency. There are seven coiled coils previously characterized in protein de novo design studies. They have been proved by previous work of Shu Xiaokun’s lab, and according to their work, HOTag3 and HOTag6 are most robust in driving protein droplet formation over a wide range of protein concentrations, so we choose them.

To design interaction modules, we tried a lot of components and we fused them to the N-terminus of HOTag3 or HOTag6. Some of them are spontaneous and some are inducible. And we can regulate them through various kinds of inducers and different intensities of promoters.

SUMO and SIM

Post-translational modifications by the small ubiquitin-like modifier (SUMO) are crucial events in cellular response to radiation and a wide range of DNA-damaging agents. Previous studies have shown that SUMO mediates protein-protein interactions by binding to a SUMO-interacting motif (SIM) on receptor proteins. And recent studies have shown that a protein with ten repeats of human SUMO3 (polySUMO) and a protein with ten repeats of SIM (polySIM) can drive interacting multivalent scaffolds in vitro. Therefore, we chose SUMO3 and SIM as a pair of interaction modules and they can spontaneously drive the formation of synthetic organelles. For plasmid construction, we did the same as FKBP and FRB. What’s more, different from inducer-mediated interactions like FKBP and FRB, we used Tet07 promoter, an inducible promoter, to initiate the expression of SIM. Then, if we add dox (the inducer of Tet07) into yeast, we will see an magical scene: yeast with two colors replaces yeast with only one color before, and red droplets colocalize with green droplets in cells through fluorescence microscope. Synthetic organelles come true!

Phytohormone

Gibberellin (GA) is a well-known phytohormone, whose perception is mediated by GID1 (GA-INSENSITIVE-DWARF1). And a key event in GA signaling is the degradation of DELLA proteins, which are negative regulators of GA response that interact with GID1 in a GA-dependent manner. Since this interaction seems to be a simple biochemical reaction that does not require additional factors, we try to do further research to make full use of it. Fortunately, it has been reported that GAI (GA-INSENSITIVE) is one of DELLA family proteins in Arabidopsis and the affinity of the GID-GA interaction can increase about 100-fold by GAI, suggesting that the GID1-GA complex is stabilized by DELLA proteins. So we chose GID1 and GAI as a pair of interaction modules. And we assembled them on to yeast plasmid as the same as the construction of FKBP and FRB and transformed them into yeast. And then, only if we add GA to the yeast can we see red droplets colocalize with green droplets in cells through fluorescence microscope. Synthetic organelles come true!

As mentioned above, interactions can be formed not only by inducers such as rapamycin and gibberellin, but also spontaneously, just as SUMO and SIM. So can we combine these two ways of interactions? To solve this problem, we did further studies about phytohormone and found ABA. Abscisic acid (ABA) is an important phytohormone that regulates plant stress responses. Proteins from the PYR-PYL-PCAR family were identified as ABA receptors. Upon binding to ABA, a PYL protein associates with type 2C protein phosphatases (PP2Cs) such as ABI1 and ABI2, inhibiting their activity. Previous structural and biochemical observations have provided insight into PYL-mediated ABA signaling and given rise to a working model. In the absence of ABA signaling, PP2Cs are fully active and PYLs exist as inactive homodimers in cells, unable to bind or inhibit PP2Cs, mainly due to the incompatible conformation of CL2loop. In response to ABA binding, the CL2 loop undergoes a conformational rearrangement to close onto the ABA-bound pocket, then, the interaction between PYLs and PP2Cs can be formed. Here we chose PYL1 and ABI1 as a pair of interaction modules. Then, we assembled them on to yeast plasmid as the same as the construction of FKBP and FRB and transformed them into yeast. Based on the interaction of PYL1 and ABI1, we can get a wonderful scene: In the absence of ABA, the synthetic organelles composed only of PYL1 appear, because of the homodimers of PYL1. And after we add ABA into yeast, ABI1 can enter the organelles with the interaction of ABI1 and PYL1, and we can see red droplets colocalize with green droplets in cells through fluorescence microscope. In this way, new components can enter the original organelles and the time of occurrence can be regulated as it is inducer-mediated regulation. So it give our designs and functions more possibilities.

Optogenetic control

The above describes a lot of components used as interaction modules, but they all have a common disadvantage——irreversible! Now, this problem can be solved be the Arabidopsis red light-inducible phytochrome (PHYB-PIF) system, which comprises the phytochrome B (PHYB) protein and the basic-helix-loop-helix (bHLH) transcription factor phytochrome interaction factor (PIF; PIF3 or PIF6). These two domains are induced to bind under far infrared and the binding is reversed within seconds of exposure to infrared light but is otherwise stable for hours in the dark. What’s more, the phytochrome system has a 10-100 larger dynamic range than the cryptochrome and LOV-based systems, and the affinity of its light-gated interaction is tighter than others. Therefore, we chose PHYB and PIF6 as a pair of interaction modules. Then, we assembled them on to yeast plasmid as the same as the construction of FKBP and FRB and transformed them into yeast. After that, synthetic organelles can form and disappear under the regulation of far infrared.

Now, we have artificially designed phase separation in cells and synthesized membraneless organelles. But how can we fulfill intended functions with synthetic organelles? Here, we propose two ideas. We reserve two sites to implement functions, which means function modules, such as enzymes in metabolism, proteins in signaling pathway, transcription factors in transcription and so on, have two sites in our designs.

Welcome——with nanobody

We introduce a magic protein, anti-GFP nanobody, which is very small (only 13-kDa, 1.5nm 2.5nm) and high-affinity (0.59nM) camelid antibody to GFP. So we can use its characteristic to improve our designs. We can fuse GFP to the C-terminus of interaction modules and to the N-terminus of HOTags, and fuse function modules to the C-terminus of anti-GFP nanobodies. Then, with the help of interaction between anti-GFP nanobodies and GFP, synthetic organelles will “welcome” function modules, expected functions can be realized. You may ask: How does anti-GFP nanobody improve the design? Firstly, it will not make the protein extremely large and will reduce the effect on the structure of function modules, which can ensure the quality of functions. Secondly, it can bring components not belonging to the original structure to synthetic organelles, which can enlarge the enrichment range of synthetic organelles. Thirdly, it is easy to regulate the expression of target proteins. So you can see, nanobodies may do better and give you a surprise!