Difference between revisions of "Team:William and Mary/Experiments"

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<title>Page Title</title>
  
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<h1>Experiments</h1>
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<p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
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<h1 style="color:black;text-align:center;">Methods & Data</h1>
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Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.
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<div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 25px' ><b>Overview</b></div>
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In the interest of promoting reproducible science, and enabling others to build off of our work, we have included both the precise methods used as well as the raw data (in csv format) for every experiment mentioned on our wiki. Experiments are split up by the page on which they are mentioned. For experiments performed with flow cytometry, .fcs files for both measurements and calibration beads (Spherotek RCP-30-5A, lot number: AB01) are included as a separate file. For all experiments, unless stated otherwise, the following is true: Circuits used were present in psb1C3, antibiotics were used in the following concentrations [Chloramphenicol: 7.5mg/mL, Kanamycin: 35mg/mL], the media used was M9 minimal media with 0.4% Casamino Acids and 0.4% Glucose (see protocols).
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<h3>What should this page contain?</h3>
 
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<li> Protocols </li>
 
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<li> Experiments </li>
 
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<li> Documentation of the development of your project </li>
 
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<div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 25px' ><b>Heat Inducible System</b></div>
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<h3>Inspiration</h3>
 
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
 
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Revision as of 20:51, 14 October 2018

Page Title

Methods & Data

Overview
In the interest of promoting reproducible science, and enabling others to build off of our work, we have included both the precise methods used as well as the raw data (in csv format) for every experiment mentioned on our wiki. Experiments are split up by the page on which they are mentioned. For experiments performed with flow cytometry, .fcs files for both measurements and calibration beads (Spherotek RCP-30-5A, lot number: AB01) are included as a separate file. For all experiments, unless stated otherwise, the following is true: Circuits used were present in psb1C3, antibiotics were used in the following concentrations [Chloramphenicol: 7.5mg/mL, Kanamycin: 35mg/mL], the media used was M9 minimal media with 0.4% Casamino Acids and 0.4% Glucose (see protocols). /div>
Heat Inducible System