Difference between revisions of "Team:William and Mary/Measurement"

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<h1 style="color:black;text-align:center;">Measurement</h1>
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<div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 25px' ><b>Overview</b></div>
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This year team William and Mary's project focused on measuring the dynamical outputs of our decoding circuit. In the process we performed time series measurements, focusing on scalability and parameter testing. Although single cell measurements using flow cytometry are our preferred way to obtain data, for much of our project we were required to test for qualitative circuit behavior using a plate reader due to the number of constructs we had to test. However 
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<h1>Measurement</h1>
 
  
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<p>There are a lot of exciting parts in the Registry, but many parts have still not been characterized. Synthetic Biology needs great measurement approaches for characterizing new parts, and efficient new methods for characterizing many parts at once. If you've done something exciting in the area of Measurement, describe it here!</p>
 
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<h3>Best Innovation in Measurement Special Prize</h3>
 
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<p>If you've done excellent work in measurement, you should consider nominating your team for this special prize. Designing great measurement approaches for characterizing new parts or developing and implementing an efficient new method for characterizing thousands of parts are good examples.
 
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To compete for the <a href="https://2018.igem.org/Judging/Awards">Best Innovation in Measurement prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a>.
 
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You must also delete the message box on the top of this page to be eligible for this prize.
 
  
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Figure 2: Relative fluorescence (fluorescence/max) measurements of the temperature activatable circuit BBa_K2680051 when grown at 30C and then activated by exposure to 37C  for the entirety of the experiment (A) or transiently (B). Dots represent the geometric mean of 3 (B) or 6 (A) distinct biological replicates (colonies) and the blue shaded region represents one geometric standard deviation above and below the mean. The grey shaded region in (B) represents the period in which the temperature was 37C. Normalized fluorescence (relative to max) was calculated by dividing each colony relative to it's maximal expression.
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<h3>Inspiration</h3>
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<p>You can look at what other teams did to get some inspiration! <br />
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Here are a few examples:</p>
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<li><a href="https://2016.igem.org/Team:Stanford-Brown">2016 Stanford-Brown</a></li>
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<li><a href="https://2016.igem.org/Team:Genspace">2016 Genspace</a></li>
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<li><a href="https://2015.igem.org/Team:William_and_Mary">2015 William and Mary</a></li>
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<li><a href="https://2014.igem.org/Team:Aachen">2014 Aachen  </a></li>
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Revision as of 15:39, 15 October 2018

Page Title

Measurement

Overview
This year team William and Mary's project focused on measuring the dynamical outputs of our decoding circuit. In the process we performed time series measurements, focusing on scalability and parameter testing. Although single cell measurements using flow cytometry are our preferred way to obtain data, for much of our project we were required to test for qualitative circuit behavior using a plate reader due to the number of constructs we had to test. However
Figure 2: Relative fluorescence (fluorescence/max) measurements of the temperature activatable circuit BBa_K2680051 when grown at 30C and then activated by exposure to 37C for the entirety of the experiment (A) or transiently (B). Dots represent the geometric mean of 3 (B) or 6 (A) distinct biological replicates (colonies) and the blue shaded region represents one geometric standard deviation above and below the mean. The grey shaded region in (B) represents the period in which the temperature was 37C. Normalized fluorescence (relative to max) was calculated by dividing each colony relative to it's maximal expression.