Difference between revisions of "Team:Austin UTexas/Parts"
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<p>When observing the parts registry, we found that no tetracycline resistance gene had Phytobrick compatibility. Using primers, we added proper MoClo standard overhangs to each part as well as providing it the proper restriction sites — BsaI and BsmBI sites. The PCR products containing these overhangs were incorporated into the Universal Acceptor backbone, BBa_P10500, which is a modified pSB1C3 backbone. <a href = "http://parts.igem.org/Part:BBa_K2657001">Link to Part Page</a></p> | <p>When observing the parts registry, we found that no tetracycline resistance gene had Phytobrick compatibility. Using primers, we added proper MoClo standard overhangs to each part as well as providing it the proper restriction sites — BsaI and BsmBI sites. The PCR products containing these overhangs were incorporated into the Universal Acceptor backbone, BBa_P10500, which is a modified pSB1C3 backbone. <a href = "http://parts.igem.org/Part:BBa_K2657001">Link to Part Page</a></p> | ||
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| − | < | + | <h4>BBa_K2657002</h4> |
<p>At the same time while looking over the parts registry, we found no origin of transfer with Phytobrick overhangs. Using the same techniques that were used to make the TetR Phytobrick, we created a the Rp4 Phytobrick, which is a level 0 OriT Phytobrick.<a href = "http://parts.igem.org/Part:BBa_K2657002">Link to Part Page</a> Here is also an example of the Rp4 part used in conjugation <a href="https://2018.igem.org/Team:Austin_UTexas/Results/Conjugations">link</a></p> | <p>At the same time while looking over the parts registry, we found no origin of transfer with Phytobrick overhangs. Using the same techniques that were used to make the TetR Phytobrick, we created a the Rp4 Phytobrick, which is a level 0 OriT Phytobrick.<a href = "http://parts.igem.org/Part:BBa_K2657002">Link to Part Page</a> Here is also an example of the Rp4 part used in conjugation <a href="https://2018.igem.org/Team:Austin_UTexas/Results/Conjugations">link</a></p> | ||
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Revision as of 19:15, 15 October 2018
Parts
BBa_K2657001
When observing the parts registry, we found that no tetracycline resistance gene had Phytobrick compatibility. Using primers, we added proper MoClo standard overhangs to each part as well as providing it the proper restriction sites — BsaI and BsmBI sites. The PCR products containing these overhangs were incorporated into the Universal Acceptor backbone, BBa_P10500, which is a modified pSB1C3 backbone. Link to Part Page
BBa_K2657002
At the same time while looking over the parts registry, we found no origin of transfer with Phytobrick overhangs. Using the same techniques that were used to make the TetR Phytobrick, we created a the Rp4 Phytobrick, which is a level 0 OriT Phytobrick.Link to Part Page Here is also an example of the Rp4 part used in conjugation link
<groupparts>iGEM18 Austin_UTexas</groupparts>