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Revision as of 01:14, 16 October 2018


Integrated Human Practices

Transformation of Assembly Plasmids into Mu Free Donors

Dr.Brian Redna of Gingko Bioworks emphasized that our kit would be limited by the ability of the plasmids to be inserted into the bacteria of interest as many can not be transformed with standard protocols. Therefore, we transformed the assembly plasmids into a strain of E. coli that can act as a plasmid donor in conjugations.

See Demonstrate Page
Figure 1: These are the results of Rice's one tube electroporation with E. coli.