Difference between revisions of "Team:UI Indonesia/Project"

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   <h3 class="w3-center"><b>MY WORK<b></h3>
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   <h5>Diphtheria is becoming a prominent issue in Indonesia as its incidence is increasing recently. It also causes various complications, leading to morbidity and mortality. We realized the urgency of fast, reliable, and cheap early detection method for diphtheria infection as one of means necessary for its eradication. Therefore, we created a chimeric between native Escherichia coli Tar chemotaxis receptor and human HB-EGF receptor so the bacterium may recognize diphtheria toxin. Moreover, we combined CheA and CheY in E. coli chemotaxis system with LuxAB and eYFP, respectively. When in contact, LuxAB and eYFP create resonance energy transfer system in which LuxAB gives its emission to eYFP. Without diphtheria toxin, CheA will be in phosphorylated state, allowing interaction with CheY and energy transfer, resulting in yellow color. Toxin binding into chimeric receptor will inhibit CheA phosphorylation, hindering interaction with CheY and energy transfer, resulting in blue color (i.e. LuxAB native color).</h5>
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   <h5>Diphtheria is an infection caused by pathogenic bacterium Corynebacterium diphtheriae. The hallmark of diphtheria infection is pseudomembrane formation in posterior pharynx, potentially leading to respiratory tract obstruction and death. In addition, the bacterium produces toxin that enters cell via interaction with heparin-binding EGF-like growth factor (HB-EGF) receptor and inhibits protein synthesis, resulting in lethal complications such as myocarditis, bleeding problem, kidney and liver necrosis.<br><br>
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  Despite being preventable and curable, incidence and mortality rate caused by diphtheria are still increasing, especially in developing countries including Indonesia. Recently, there has been diphtheria outbreak affecting major provinces in Indonesia, mainly in unvaccinated children and adults. Therefore, there are two issues to be highlighted: (1) lack of early detection and prompt treatment of diphtheria infection and (2) lack of awareness regarding diphtheria and vaccination among Indonesian people.</h5>
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   <h5>We, fourteen undergraduate students from various backgrounds in iGEM University of Indonesia (UI) 2018 team, are collaborating in attempt to overcome these problems. We plan to develop an alternative method for detecting diphtheria toxin by integrating native Escherichia coli chemotaxis system with heparin-binding EGF-like growth factor (HB-EGF) receptor and fluorescence resonance energy transfer (FRET) system, comprising of LuxAB and enhanced yellow fluorescence protein (eYFP). Furthermore, we plan to raise awareness regarding diphtheria and vaccination among people around us by means of charities and social works. For further information about our project, human practices, collaboration with other iGEM teams, and many others, feel free to navigate our Wiki pages.</h5>
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Revision as of 15:00, 25 June 2018

Home
Project ⯆
Overview Our Project Results and Discussions
Modeling Interlab Studies Human Practices Collaborations Team Attributions

MY WORK

Diphtheria is becoming a prominent issue in Indonesia as its incidence is increasing recently. It also causes various complications, leading to morbidity and mortality. We realized the urgency of fast, reliable, and cheap early detection method for diphtheria infection as one of means necessary for its eradication. Therefore, we created a chimeric between native Escherichia coli Tar chemotaxis receptor and human HB-EGF receptor so the bacterium may recognize diphtheria toxin. Moreover, we combined CheA and CheY in E. coli chemotaxis system with LuxAB and eYFP, respectively. When in contact, LuxAB and eYFP create resonance energy transfer system in which LuxAB gives its emission to eYFP. Without diphtheria toxin, CheA will be in phosphorylated state, allowing interaction with CheY and energy transfer, resulting in yellow color. Toxin binding into chimeric receptor will inhibit CheA phosphorylation, hindering interaction with CheY and energy transfer, resulting in blue color (i.e. LuxAB native color).
Team UI Indonesia
igemui2018@gmail.com