Difference between revisions of "Team:SHSBNU China/InterLab"

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<h3>a. Plasmids Used</h3>
 
<h3>a. Plasmids Used</h3>
 
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<div class="content">
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<li class="content_list">Negative control  BBa_R0040      (provided in kit plate)</li>
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<li class="content_list"><p>Negative control  BBa_R0040      (provided in kit plate)</p></li>
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<li class="content_list">Positive control  BBa_I20270    (provided in kit plate)</li>
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<li class="content_list"><p>Positive control  BBa_I20270    (provided in kit plate)</p></li>
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<li class="content_list">Test Device 1      BBa_J364000    (provided in kit plate)</li>
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<li class="content_list"><p>Test Device 1      BBa_J364000    (provided in kit plate)</p></li>
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<li class="content_list">Test Device 2      BBa_J364001    (provided in kit plate)</li>
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<li class="content_list"><p>Test Device 2      BBa_J364001    (provided in kit plate)</p></li>
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<li class="content_list">Test Device 3      BBa_J36400    (provided in kit plate)</li>
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<li class="content_list"><p>Test Device 3      BBa_J36400    (provided in kit plate)</p></li>
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<li class="content_list">Test Device 4      BBa_J364007    (provided in kit plate)</li>
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<li class="content_list"><p>Test Device 4      BBa_J364007    (provided in kit plate)</p></li>
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<li class="content_list">Test Device 5      BBa_J364008    (provided in kit plate) </li>
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<li class="content_list"><p>Test Device 5      BBa_J364008    (provided in kit plate)</p></li>
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<li class="content_list">Test Device 6      BBa_J364009    (provided in kit plate)</li>
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<li class="content_list"><p>Test Device 6      BBa_J364009    (provided in kit plate)</p></li>
 
</div>
 
</div>
 
<h3>b. Strain used</h3>
 
<h3>b. Strain used</h3>

Revision as of 14:53, 16 October 2018

Section Sample

InterLab

I. Background

“All of 2018 iGEM teams are invited and encouraged to participate in the Fifth International InterLaboratory Measurement Study in synthetic biology.” Our team took part in this study which is aimed to figure out and correct the sources of systematic variability in synthetic biology measurements. The main task was to quantify expression of GFP in common, comparable or absolute units. In our case, we measured fluorescence using plate reader.

II. Design

Fluorescence is widely used as a proxy for promoter activity by expressing fluorescent proteins such as green fluorescent protein (GFP). Despite this is an indirect measurement, it provides a useful insight into expression levels and has significant advantage that it could be a real-time monitor without disrupting cells.

Fluorescence/OD600 is routinely used to give an adjustment of the relative expression per cell.

III. Material and Methods

a. Plasmids Used

  • Negative control BBa_R0040 (provided in kit plate)

  • Positive control BBa_I20270 (provided in kit plate)

  • Test Device 1 BBa_J364000 (provided in kit plate)

  • Test Device 2 BBa_J364001 (provided in kit plate)

  • Test Device 3 BBa_J36400 (provided in kit plate)

  • Test Device 4 BBa_J364007 (provided in kit plate)

  • Test Device 5 BBa_J364008 (provided in kit plate)

  • Test Device 6 BBa_J364009 (provided in kit plate)

    • b. Strain used

    c. Machines

    d. Software

    f. Results

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