Difference between revisions of "Team:William and Mary/Notebook"

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·        Continued cloning 3G parts onto our standard WM Pad backbone
 
·        Redesigned primers for cloning 3G promoter parts, as previous ones were faulty
 
·        Received results from sequencing, confirmed parts
 
·        Beginning to assemble circuits using 3G assembly
 
·        Making inducible reporter construct and an mf-Lon based IFFL
 
 
  
 
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Revision as of 16:33, 16 October 2018

Notebook

Week 1: 180528-180604
  • Began cloning 3G parts based off the library sent from the Murray lab
  • Researched literature on dual inducible promoters
  • Sent out several cloned 3G parts for sequencing
  • Planned out several IFFLs to test.
Week 2: 180604-180610
  • Continued cloning 3G parts onto our standard WM Pad backbone
  • Redesigned primers for cloning 3G promoter parts, as previous ones were faulty
  • Received results from sequencing, confirmed parts
  • Beginning to assemble circuits using 3G assembly
  • Making inducible reporter construct and an mf-Lon based IFFL