Difference between revisions of "Team:KCL UK/Safety"

Line 1: Line 1:
−
{{Example}}
+
{{:Team:KCL_UK/Templates/Header}}
 
<html>
 
<html>
 +
    <head>
  
 +
    <meta charset="UTF-8">
 +
    <meta name="viewport" content="width= device-width, initial-scale=1">
 +
    <title>KCL iGEM</title>
  
−
<div class="column full_size">
 
  
−
<h1> Safety </h1>
+
    <style>
−
<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
+
  
−
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
+
    @import url('https://fonts.googleapis.com/css?family=Raleway');
 +
    #top_title {display:none;}
  
−
</div>
 
  
  
−
<div class="column two_thirds_size">
+
  #HQ_page p {
−
<h3>Safe Project Design</h3>
+
font-family: 'Raleway', sans-serif;
 +
font-size: 1em;
 +
text-align: center;
  
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
}
  
−
<ul>
+
    #HQ_page h1,h2,h3 {
−
<li>Choosing a non-pathogenic chassis</li>
+
font-family: 'Raleway', sans-serif;
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
font-weight: 300;
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
}
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
−
</ul>
+
  
−
</div>
+
      #content, #HQ_page {
 +
            animation : none;
 +
    animation-delay : 0;
 +
    animation-direction : normal;
 +
    animation-duration : 0;
 +
    animation-fill-mode : none;
 +
    animation-iteration-count : 1;
 +
    animation-name : none;
 +
    animation-play-state : running;
 +
    animation-timing-function : ease;
 +
    backface-visibility : visible;
 +
    background : 0;
 +
    background-attachment : scroll;
 +
    background-clip : border-box;
 +
    background-color : transparent;
 +
    background-image : none;
 +
    background-origin : padding-box;
 +
    background-position : 0 0;
 +
    background-position-x : 0;
 +
    background-position-y : 0;
 +
    background-repeat : repeat;
 +
    background-size : auto auto;
 +
    border : 0;
 +
    border-style : none;
 +
    border-width : medium;
 +
    border-color : inherit;
 +
    border-bottom : 0;
 +
    border-bottom-color : inherit;
 +
    border-bottom-left-radius : 0;
 +
    border-bottom-right-radius : 0;
 +
    border-bottom-style : none;
 +
    border-bottom-width : medium;
 +
    border-collapse : separate;
 +
    border-image : none;
 +
    border-left : 0;
 +
    border-left-color : inherit;
 +
    border-left-style : none;
 +
    border-left-width : medium;
 +
    border-radius : 0;
 +
    border-right : 0;
 +
    border-right-color : inherit;
 +
    border-right-style : none;
 +
    border-right-width : medium;
 +
    border-spacing : 0;
 +
    border-top : 0;
 +
    border-top-color : inherit;
 +
    border-top-left-radius : 0;
 +
    border-top-right-radius : 0;
 +
    border-top-style : none;
 +
    border-top-width : medium;
 +
    bottom : auto;
 +
    box-shadow : none;
 +
    box-sizing : content-box;
 +
    caption-side : top;
 +
    clear : none;
 +
    clip : auto;
 +
    color : inherit;
 +
    columns : auto;
 +
    column-count : auto;
 +
    column-fill : balance;
 +
    column-gap : normal;
 +
    column-rule : medium none currentColor;
 +
    column-rule-color : currentColor;
 +
    column-rule-style : none;
 +
    column-rule-width : none;
 +
    column-span : 1;
 +
    column-width : auto;
 +
    content : normal;
 +
    counter-increment : none;
 +
    counter-reset : none;
 +
    cursor : auto;
 +
    direction : ltr;
 +
    display : inline;
 +
    empty-cells : show;
 +
    float : none;
 +
    font : normal;
 +
    font-family: 'Raleway', sans-serif;
 +
    font-size : medium;
 +
    font-style : normal;
 +
    font-variant : normal;
 +
    font-weight : normal;
 +
    height : auto;
 +
    hyphens : none;
 +
    left : auto;
 +
    letter-spacing : normal;
 +
    line-height : normal;
 +
    list-style : none;
 +
    list-style-image : none;
 +
    list-style-position : outside;
 +
    list-style-type : disc;
 +
    margin : 0;
 +
    margin-bottom : 0;
 +
    margin-left : 0;
 +
    margin-right : 0;
 +
    margin-top : 0;
 +
    max-height : none;
 +
    max-width : none;
 +
    min-height : 0;
 +
    min-width : 0;
 +
    opacity : 1;
 +
    orphans : 0;
 +
    outline : 0;
 +
    outline-color : invert;
 +
    outline-style : none;
 +
    outline-width : medium;
 +
    overflow : visible;
 +
    overflow-x : visible;
 +
    overflow-y : visible;
 +
    padding : 0;
 +
    padding-bottom : 0;
 +
    padding-left : 0;
 +
    padding-right : 0;
 +
    padding-top : 0;
 +
    page-break-after : auto;
 +
    page-break-before : auto;
 +
    page-break-inside : auto;
 +
    perspective : none;
 +
    perspective-origin : 50% 50%;
 +
    position : static;
 +
    /* May need to alter quotes for different locales (e.g fr) */
 +
    quotes : '\201C' '\201D' '\2018' '\2019';
 +
    right : auto;
 +
    tab-size : 8;
 +
    table-layout : auto;
 +
    text-align : inherit;
 +
    text-align-last : auto;
 +
    text-decoration : none;
 +
    text-decoration-color : inherit;
 +
    text-decoration-line : none;
 +
    text-decoration-style : solid;
 +
    text-indent : 0;
 +
    text-shadow : none;
 +
    text-transform : none;
 +
    top : auto;
 +
    transform : none;
 +
    transform-style : flat;
 +
    transition : none;
 +
    transition-delay : 0s;
 +
    transition-duration : 0s;
 +
    transition-property : none;
 +
    transition-timing-function : ease;
 +
    unicode-bidi : normal;
 +
    vertical-align : baseline;
 +
    visibility : visible;
 +
    white-space : normal;
 +
    widows : 0;
 +
    width : auto;
 +
    word-spacing : normal;
 +
    z-index : auto;
 +
      }
  
−
<div class="column third_size">
+
h1 {
−
<h3>Safe Lab Work</h3>
+
display: block;
 +
font-size: 2em;
 +
margin-bottom: 0.67em;
 +
margin-left: 0;
 +
margin-right: 0;
 +
  }
  
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
  a:hover {text-decoration:none;}
 +
a:visited {
 +
  color:red;
 +
  text-decoration:none;
 +
}
  
 +
  .dropdown-nav-buttonmasks:visited {
 +
    color:white;
 +
      text-decoration: none;
 +
    }
 +
.standalonenav:visited {
 +
  color:white;
 +
  text-decoration: none;
 +
}
  
−
<h3>Safe Shipment</h3>
 
  
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
+
 
−
</div>
+
        * {
 +
            box-sizing: border-box;
 +
            font-family: 'Raleway', sans-serif;
 +
        }
 +
 
 +
 
 +
        body{
 +
        margin: 0;
 +
    padding: 0;
 +
        font-family: 'Raleway', sans-serif;
 +
        text-align: center;
 +
        }
 +
 
 +
        img {
 +
            max-width: 100%;
 +
            height: auto;
 +
        }
 +
 
 +
        .container {
 +
            width: 95%;
 +
            margin: 0 auto;
 +
        }
 +
        .fixcontainer {
 +
          width:100%;
 +
          margin: 0 auto;
 +
        }
 +
 
 +
        /*typography------------------------------*/
 +
        .title {
 +
            font-weight: 900;
 +
            font-weight: bold;
 +
            font-size: 3.2rem;
 +
            margin-bottom: 1em;
 +
            padding-bottom: 2em;
 +
            text-emphasis: bold;
 +
        }
 +
        .smallertitle {
 +
          font-weight: 900;
 +
          font-weight: bold;
 +
          font-size: 1.4rem;
 +
          margin-bottom: 1em;
 +
          padding-top: 3.1em;
 +
          padding-bottom: 0em;
 +
          text-emphasis: bold;
 +
 
 +
        }
 +
        h1 {
 +
            font-weight: 300;
 +
            font-size: 2.5em;
 +
            text-transform: capitalize;
 +
          }
 +
        .title span {
 +
            margin-top: 1em;
 +
            font-weight: 200;
 +
            display: block;
 +
            font-size: .9em;
 +
        }
 +
 
 +
 
 +
        /*buttonmasks -------------------------------*/
 +
        .buttonmask {
 +
            display: inline-block;
 +
            font-size: 1.25rem;
 +
            text-decoration: none;
 +
            text-transform: uppercase;
 +
            border-width: 2px;
 +
            border-style: solid;
 +
            padding: .5em 1.75em;
 +
 
 +
        }
 +
  .buttonmask}
 +
 
 +
        .buttonmask-small {
 +
            font-size: .9rem;
 +
            font-weight: 700;
 +
        }
 +
 
 +
        .buttonmask-accent {
 +
            color: #FF0000;
 +
            border-color: #FF0000;
 +
        }
 +
 
 +
        .buttonmask-accent:hover {
 +
        background: #FF0000;
 +
        color: black;
 +
        }
 +
 
 +
 
 +
        /*header------------------------------*/
 +
 
 +
        header {
 +
            background-color: rgba(0,0,0,.75);
 +
            position: absolute;
 +
            left: 0;
 +
            right: 0;
 +
            max-height: 7em;
 +
        }
 +
 
 +
        nav ul {
 +
            margin: 0;
 +
            padding: 0;
 +
            list-style: none;
 +
            float: right;
 +
            margin-right: 5em!important;
 +
            margin-top: 2em;
 +
            position: relative;
 +
        }
 +
 
 +
        nav li {
 +
            display: inline-block;
 +
            margin: 1em;
 +
            position: relative;
 +
        }
 +
 
 +
        .nav-buttonmasks {
 +
            font-weight: 900;
 +
            text-decoration: none;
 +
            text-transform: uppercase;
 +
            font-size: 0.95rem;
 +
            padding: .5em;
 +
            color: #FFF;
 +
            position: relative;
 +
            padding-bottom: 3em;
 +
        }
 +
 
 +
        .nav-buttonmasks:hover {color: #FF0000;}
 +
        .dropdown-nav-buttonmasks:hover {color: #FF0000;}
 +
 
 +
        .dropdown-nav-buttonmasks {
 +
            font-weight: 900;
 +
            text-decoration: none;
 +
            text-transform: uppercase;
 +
            font-size: 0.95rem;
 +
            padding: .5em;
 +
            color: #FFF;
 +
            display: none;
 +
            background-color:  rgba(0,0,0,.75);
 +
            z-index: 10;
 +
            width: 10em;
 +
            margin-left: -3em;
 +
        }
 +
        #navadjust {
 +
          margin-left:-0.3em;
 +
        }
 +
 
 +
        .dropdownone {
 +
          margin-top: 2.58em!important;
 +
        }
 +
        .dropdowntwo {
 +
            margin-top: 4.75em!important;
 +
        }
 +
        .dropdownthree {
 +
          margin-top: 6.93em!important;
 +
        }
 +
        .dropdownfour {
 +
            margin-top: 9.1em!important;
 +
        }
 +
        .dropdownfive {
 +
            margin-top: 11.28em!important;
 +
        }
 +
        .dropdownsix {
 +
            margin-top: 13.46em!important;
 +
        }
 +
        .dropdownseven {
 +
          margin-top: 15.66em!important;
 +
}
 +
        .dropdowneight {
 +
          margin-top: 17.88em!important;
 +
}
 +
 
 +
        .nav-buttonmasks:hover .dropdown-nav-buttonmasks{
 +
            display: block;
 +
            position: absolute;
 +
        }
 +
        .logo {
 +
            height: 90px;
 +
            width: 90px;
 +
            float: left;
 +
            margin-left: 6em;
 +
            margin-top: .5em;
 +
        }
 +
.uselessspacer {
 +
          height: 16vh;
 +
          background-color: black;
 +
        }
 +
 
 +
        /*top------------------------------*/
 +
        .top {
 +
          background-color: black;
 +
          height: 19vh;
 +
        }
 +
        .line {
 +
                width: 30%;
 +
                height: 1.5px;
 +
                margin: auto;
 +
            background: red;
 +
        }
 +
        .top h1 {
 +
          color: red!important;
 +
        }
 +
 
 +
        /*content------------------------------*/
 +
        .divisiontitletext {
 +
          margin: auto;
 +
 
 +
          font-size: 2em!important;
 +
        }
 +
        .leftline {
 +
          float: left;
 +
          width: 37%;
 +
          height: 1.5px;
 +
          background: red;
 +
          margin-left: -33em!important;
 +
          margin-top: 5em;
 +
        }
 +
        .divisiontitle {
 +
          width: 100%;
 +
          height: 7em;
 +
          background-color: black;
 +
          color: white;
 +
          margin: auto;
 +
        }
 +
        .collab {
 +
          width: 100%;
 +
          background-color: black;
 +
          outline: 2px solid red;
 +
          outline-offset: -2em;
 +
        }
 +
        #first {
 +
          height: 60em;
 +
        }
 +
        #second {
 +
          height: 30em;
 +
        }
 +
        #third {
 +
          height: 20em;
 +
        }
 +
        .longname {
 +
          font-size: 2em;
 +
          color: white;
 +
          background-color: black;
 +
          width: 80vw;
 +
          margin-top: .6em;
 +
          z-index: 1;
 +
          position: absolute;
 +
          margin: auto;
 +
          margin-left: 10vw;
 +
          padding-top: .4em;
 +
        }
 +
        #secondlongname {
 +
          width: 40vw;
 +
          margin-left: 30vw;
 +
        }
 +
        .smallname {
 +
          font-size: 2em;
 +
          color: red;
 +
          background-color: black;
 +
          width: 18vw;
 +
          margin-top: .4em;
 +
          z-index: 1
 +
;
 +
          position: absolute;
 +
        }
 +
        .leftname {
 +
          float:left;
 +
          margin-left: 3em!important;
 +
        }
 +
        .rightname {
 +
          float:right;
 +
          margin-left: 60vw;
 +
        }
 +
 
 +
        .description {
 +
          text-align: left;
 +
          background-color: black;
 +
          padding-top: 2em;
 +
          width: 80vw;
 +
          margin: auto;
 +
          font-size: 1.2em;
 +
          color: white;
 +
        }
 +
        .descriptionfix {
 +
          text-align: left;
 +
          background-color: black;
 +
          padding-top: 2em;
 +
          width: 80vw;
 +
          margin: auto;
 +
          font-size: 1.2em;
 +
          color: white;
 +
        }
 +
        .leftdescription {
 +
          float: left;
 +
          margin-left: 10vw;
 +
          width: 40vw;
 +
          display: inline-block;
 +
          background-color: black;
 +
          padding-top: 8em;
 +
 
 +
        }
 +
        .ourwiki {
 +
          display: inline-block;
 +
          width: 30vw;
 +
          background-color: black;
 +
          float: left;
 +
          margin-left: 8vw;
 +
        }
 +
        .firstdescription{
 +
          padding-top: 8em;
 +
        }
 +
        .seconddescription {
 +
          padding-top: 3em;
 +
        }
 +
        .linedescadjust {
 +
          margin-top: 2em;
 +
          margin
 +
        }
 +
        .bullet {
 +
          margin-top: 1em;
 +
          color: white;
 +
          font-size: 1.1em;
 +
          text-align: left;
 +
        }
 +
        .ourwikidesc {
 +
          margin-bottom: 1em;
 +
        }
 +
        .test {
 +
          height: 25em;
 +
          background-color: black;
 +
        }
 +
        .collabinfographic {
 +
          width: 70vw;
 +
          height: 130em;
 +
          background-image: url(https://static.igem.org/mediawiki/2018/0/0d/T--KCL_UK--collaborationinfographic.png);
 +
          background-size:contain;
 +
          background-repeat: no-repeat;
 +
            margin: auto;
 +
        }
 +
        .spacer {
 +
          background-color: black;
 +
          height: 3em!important;
 +
        }
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
    </style>
 +
 
 +
 
 +
 
 +
 
 +
    </head>
 +
 
 +
 
 +
    <body>
 +
 
 +
 
 +
 
 +
    <div class="uselessspacer"></div>
 +
 
 +
    <section class="top">
 +
      <h1>Safety</h1>
 +
      <div class="line"></div>
 +
    </section>
 +
 
 +
    <section class="collabcontent">
 +
      <div class="collab" id="first">
 +
        <div class="firstdescription description">Laboratory safety is paramount in ensuring a productive work environment. Prior to entering the lab to begin our project, our lab team attended two laboratory safety presentations where they were briefed and trained in maintaining safe practice in the lab.</div>
 +
          <div class="line linedescadjust"></div>
 +
          <div class="seconddescription description">With regards to our project, The RNAs we used in our project are well categorised and do not pose a threat to the public nor our lab workers. Our model organism used, E. Coli is typically low risk, falling into ‘Risk group 1’. E. coli typically inhabits the human colon, via ingestion however under good lab safety practice, this shouldn’t be an issue. E. Coli bind the mucosal colon surface firstly by the production of specific glycocalyx or fimbriae from the bacterium surface. Glycocalyx bind specific lectins on the enterocyte lining of the human colon. E. coli K12 is defective in several cell wall characteristics.</div>
 +
          <div class="line linedescadjust"></div>
 +
          <div class="seconddescription description">It's outer membrane has a defective lipopolysaccharide core which interrupts attachment of O- antigen polysaccharide side chains. It does not contain glycocalyx needed to attach to mucosal surfaces of the human colon due to altered O -antigen properties and K12 do not express capsular (K) antigens which are important in colonization and virulence. (Kuhnert et al 1995) Therefore, cannot colonise the human colon under normal conditions and K12 cannot under normal conditions colonize the GI tract of animals, negative results noted in mice, pigs, chickens and calves.
 +
          </div>
 +
          <div class="line linedescadjust"></div>
 +
          <div class="seconddescription description">K12 has not shown to have negative effects on plants (U.S. Department of Agriculture) Therefore, K12 is very unlikely to pose a hazard to animals, plants or microorganisms. Our plasmids do not pose any threat in toxicity or harmfulness and are adequately safe for transport and storage.
 +
          </div>
 +
 
 +
 
 +
 
 +
      </div>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
    </section>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
    </body>
  
  
 
</html>
 
</html>

Revision as of 18:02, 16 October 2018

KCL iGEM

KCL iGEM

Safety

Laboratory safety is paramount in ensuring a productive work environment. Prior to entering the lab to begin our project, our lab team attended two laboratory safety presentations where they were briefed and trained in maintaining safe practice in the lab.
With regards to our project, The RNAs we used in our project are well categorised and do not pose a threat to the public nor our lab workers. Our model organism used, E. Coli is typically low risk, falling into ‘Risk group 1’. E. coli typically inhabits the human colon, via ingestion however under good lab safety practice, this shouldn’t be an issue. E. Coli bind the mucosal colon surface firstly by the production of specific glycocalyx or fimbriae from the bacterium surface. Glycocalyx bind specific lectins on the enterocyte lining of the human colon. E. coli K12 is defective in several cell wall characteristics.
It's outer membrane has a defective lipopolysaccharide core which interrupts attachment of O- antigen polysaccharide side chains. It does not contain glycocalyx needed to attach to mucosal surfaces of the human colon due to altered O -antigen properties and K12 do not express capsular (K) antigens which are important in colonization and virulence. (Kuhnert et al 1995) Therefore, cannot colonise the human colon under normal conditions and K12 cannot under normal conditions colonize the GI tract of animals, negative results noted in mice, pigs, chickens and calves.
K12 has not shown to have negative effects on plants (U.S. Department of Agriculture) Therefore, K12 is very unlikely to pose a hazard to animals, plants or microorganisms. Our plasmids do not pose any threat in toxicity or harmfulness and are adequately safe for transport and storage.