Difference between revisions of "Team:NUDT CHINA/Model/Deduction"
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| − | <h1 style="font-size: 40px"> | + | <h1 style="font-size: 40px">Deduction</h1> |
| − | <h2 style="font-size: 32px;margin-bottom: 10px;margin-top: 15px;"> | + | <h2 style="font-size: 32px;margin-bottom: 10px;margin-top: 15px;">Phenotype</h2> |
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<div class="col-md-push-1 col-md-10" alt = "this is a paragraph model"> | <div class="col-md-push-1 col-md-10" alt = "this is a paragraph model"> | ||
| − | <p> | + | <p>Having completed parameters simulation（插入链接到拟合），we can now use our model to make assessments about GFP and ErbB3 with the confidence that we have maximized our potential to obtain insights that will be directly relevant to the use of our trim21-antibody system. |
| + | To investigate the relation between antibody phenotype and time as well as the concentration of plasmid dosage, we respectively introduced equations between the phenotype and the corresponding protein concentration into our model. | ||
| + | </p> | ||
<p> | <p> | ||
| − | + | For gfp, the relationship between the general fluorescence intensity and the concentration of the fluorescent substance is . In the wetlab,as the concentration of gfp is counted in nanomole and the content is very low,it can be considered that the relationship at this time is in accordance with .At this time, the fluorescence intensity is proportional to the concentration of the fluorescent substance.Simultaneously, we obtained a large number of images of gfp fluorescence intensity at different time points through wetlab. These images were expressed by imagej to quantify their fluorescence intensity. Then, we use Excel to process the acquired data. For example, the shooting parameters (background brightness, contrast) of images obtained under different conditions is adjusted to reduce noise. Finally, we used the previous construction to obtain the relationship between plasmid concentration and fluorescence intensity decay. The relationship between plasmid concentration and fluorescence intensity decay was constructed using the previous method to analyze. | |
</p> | </p> | ||
</div> | </div> | ||
Revision as of 18:12, 16 October 2018
Designed Protein Degradation Method Based on
Trim21 And Nanobody
Deduction
Phenotype
Having completed parameters simulation（插入链接到拟合），we can now use our model to make assessments about GFP and ErbB3 with the confidence that we have maximized our potential to obtain insights that will be directly relevant to the use of our trim21-antibody system. To investigate the relation between antibody phenotype and time as well as the concentration of plasmid dosage, we respectively introduced equations between the phenotype and the corresponding protein concentration into our model.
For gfp, the relationship between the general fluorescence intensity and the concentration of the fluorescent substance is . In the wetlab,as the concentration of gfp is counted in nanomole and the content is very low,it can be considered that the relationship at this time is in accordance with .At this time, the fluorescence intensity is proportional to the concentration of the fluorescent substance.Simultaneously, we obtained a large number of images of gfp fluorescence intensity at different time points through wetlab. These images were expressed by imagej to quantify their fluorescence intensity. Then, we use Excel to process the acquired data. For example, the shooting parameters (background brightness, contrast) of images obtained under different conditions is adjusted to reduce noise. Finally, we used the previous construction to obtain the relationship between plasmid concentration and fluorescence intensity decay. The relationship between plasmid concentration and fluorescence intensity decay was constructed using the previous method to analyze.
This is the sign of the famous game!
This is the sign of the famous game!
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Maybe you want to write something interesting thing here! Someone famous in Source Title
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This is the sign of the famous game!
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| name | city | |
|---|---|---|
| Tanmay | Bangalore | 560001 |
| Sachin | Mumbai | 400003 |
| Uma | Pune | 411027 |
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