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<p2>This is a redox-cycling molecule produced by Pseudomonas aeuriginosa. In its oxidised form it can activate gene transcription of redox-signalling pathways, either directly or by oxidative stress. In normal aerobic conditions it is oxidised. | <p2>This is a redox-cycling molecule produced by Pseudomonas aeuriginosa. In its oxidised form it can activate gene transcription of redox-signalling pathways, either directly or by oxidative stress. In normal aerobic conditions it is oxidised. | ||
</p2> | </p2> | ||
− | + | <img class="center" src="https://static.igem.org/mediawiki/2018/4/4b/T--Imperial_College--Pyostructure.png" alt="" width="20%";> | |
<h4>Ferrocyanide/Ferricyanide:</h4> | <h4>Ferrocyanide/Ferricyanide:</h4> | ||
<p2>These molecules are well known redox mediators. The oxidised form (ferrocyanide) can draw electrons from the cells quinone pool. This amplifies the activity of redox-cycling molecules like pyocyanin. When the reduced form (ferricyanide) is present the opposite is true.</p2> | <p2>These molecules are well known redox mediators. The oxidised form (ferrocyanide) can draw electrons from the cells quinone pool. This amplifies the activity of redox-cycling molecules like pyocyanin. When the reduced form (ferricyanide) is present the opposite is true.</p2> | ||
− | + | <img class="center" src="https://static.igem.org/mediawiki/2018/3/38/T--Imperial_College--Ferrostructure.png" alt="" width="20%";> | |
+ | <img class="center" src="https://static.igem.org/mediawiki/2018/3/38/T--Imperial_College--Ferristructure.png" alt="" width="20%";> | ||
<h4>Sodium Sulfite:</h4> | <h4>Sodium Sulfite:</h4> | ||
<p2>This is an oxygen scavenger as it removes oxygen from solution. This allows for pyocyanin to be maintained in a reduced form but does not diminish GFP fluorescence, suggesting cells remain in aerobic conditions.</p2> | <p2>This is an oxygen scavenger as it removes oxygen from solution. This allows for pyocyanin to be maintained in a reduced form but does not diminish GFP fluorescence, suggesting cells remain in aerobic conditions.</p2> | ||
</div> | </div> | ||
− | + | <img class="center" src="https://2018.igem.org/File:T--Imperial_College--Naso3structure.png" alt="" width="20%";> | |
<h3 id="biomodule">Biological Module Design</h3> | <h3 id="biomodule">Biological Module Design</h3> | ||
Revision as of 05:51, 17 October 2018
Design
Design Overview
Electrochemical Module Design
![](https://static.igem.org/mediawiki/2018/e/ea/T--Imperial_College--Electrochemicalmodule.png)
Potentiostat:
Electrode or Electrode Array:
Pyocyanin:
![](https://static.igem.org/mediawiki/2018/4/4b/T--Imperial_College--Pyostructure.png)
Ferrocyanide/Ferricyanide:
![](https://static.igem.org/mediawiki/2018/3/38/T--Imperial_College--Ferrostructure.png)
![](https://static.igem.org/mediawiki/2018/3/38/T--Imperial_College--Ferristructure.png)
Sodium Sulfite:
![](https://2018.igem.org/File:T--Imperial_College--Naso3structure.png)
Biological Module Design
![](https://static.igem.org/mediawiki/2018/b/b9/T--Imperial_College--BiologicalModule.png)
SoxR:
pSoxS:
Quinone Pool:
PixCell Electrogenetic System Design
![](https://static.igem.org/mediawiki/2018/1/13/T--Imperial_College--0.3SystemPixcell.png)
![](https://static.igem.org/mediawiki/2018/5/58/T--Imperial_College--0.5SystemPixcell.png)