Difference between revisions of "Team:Imperial College/Measurement"
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| − | <During PixCell project we discovered that conventional DNA assembly methods not always adapt to rapid troubleshooting of some construct parts. As our initial construct was open for improvement to improve our model we decided to create a library of transcription factors and promoters and try creating new constructs with them. So, we started looking for a more modular method that would allow us to easily vary 1 or 2 components in a construct keeping everything else the same in order to build our library. We found that BASIC was the perfect technology for this.> | + | <p>During PixCell project we discovered that conventional DNA assembly methods not always adapt to rapid troubleshooting of some construct parts. As our initial construct was open for improvement to improve our model we decided to create a library of transcription factors and promoters and try creating new constructs with them. So, we started looking for a more modular method that would allow us to easily vary 1 or 2 components in a construct keeping everything else the same in order to build our library. We found that BASIC was the perfect technology for this.</p> |
<BASIC is a modular assembly method in which the different construct parts are made modular linking them to pairs of oligos called linkers. Each linker assembles a part to the previous and subsequent parts. This way it allows to build complex genetic circuits and multipart combinatorial assemblies. Besides, linker oligos are large enough to allow encoding an RBS sequence inside, this enables RBS troubleshooting of a construct trying different combinations of RBS without having to change the full structure of the assembly. Furthermore, BASIC includes linkers capable of fusing proteins, making it the best method capable of rapid assembly and characterisation of protein fusion libraries.> | <BASIC is a modular assembly method in which the different construct parts are made modular linking them to pairs of oligos called linkers. Each linker assembles a part to the previous and subsequent parts. This way it allows to build complex genetic circuits and multipart combinatorial assemblies. Besides, linker oligos are large enough to allow encoding an RBS sequence inside, this enables RBS troubleshooting of a construct trying different combinations of RBS without having to change the full structure of the assembly. Furthermore, BASIC includes linkers capable of fusing proteins, making it the best method capable of rapid assembly and characterisation of protein fusion libraries.> | ||
Revision as of 17:25, 17 October 2018
During PixCell project we discovered that conventional DNA assembly methods not always adapt to rapid troubleshooting of some construct parts. As our initial construct was open for improvement to improve our model we decided to create a library of transcription factors and promoters and try creating new constructs with them. So, we started looking for a more modular method that would allow us to easily vary 1 or 2 components in a construct keeping everything else the same in order to build our library. We found that BASIC was the perfect technology for this.
BASIC Method
