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<h4>Pyocyanin:</h4> | <h4>Pyocyanin:</h4> | ||
− | <p2>This is a redox-cycling molecule produced by <i>Pseudomonas aeuriginosa</i>. In its oxidised form it applies oxidative stress to a cell, activating redox-signalling pathways in the process. In normal aerobic conditions it is oxidised. | + | <p2>This is a redox-cycling molecule produced by <i>Pseudomonas aeuriginosa</i>. In its oxidised form it applies oxidative stress to a cell, activating redox-signalling pathways in the process. In normal aerobic conditions it is oxidised. |
+ | <img src="https://static.igem.org/mediawiki/2018/4/4b/T--Imperial_College--Pyostructure.png" alt="" width="5%"; > | ||
</p2> | </p2> | ||
<h4>Ferrocyanide/Ferricyanide:</h4> | <h4>Ferrocyanide/Ferricyanide:</h4> | ||
− | <p2>These molecules are well known redox mediators, meaning, they alter the redox-state of the cell. When the reduced form (ferricyanide) is present a reducing cellular environment is created, preventing the induction of redox-sensing gene circuits. When the oxidised form (ferrocyanide) is present an oxidising cellular environment is creating, permitting activation of redox-sensing gene circuit. .</p2> | + | <p2>These molecules are well known redox mediators, meaning, they alter the redox-state of the cell. When the reduced form (ferricyanide) is present a reducing cellular environment is created, preventing the induction of redox-sensing gene circuits. When the oxidised form (ferrocyanide) is present an oxidising cellular environment is creating, permitting activation of redox-sensing gene circuit. |
+ | <img src="https://static.igem.org/mediawiki/2018/3/38/T--Imperial_College--Ferrostructure.png" alt="" width="5%"; >.</p2> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/1/17/T--Imperial_College--Ferristructure.png" alt="" width="5%"; >.</p2> | ||
<h4>Sodium Sulfite:</h4> | <h4>Sodium Sulfite:</h4> | ||
Line 44: | Line 47: | ||
Supposed Electrochemical Module Mechanism: | Supposed Electrochemical Module Mechanism: | ||
Sulfite removes oxygen from solution allowing pyocyanin to be maintained in a reduced state. A potential of +0.5V generates oxidised pyocyanin and ferricyanide. Ferricyanide pushes the cell into an oxidising condition, allowing pyocyanin to remain oxidised and activate gene expression of a redox sensing gene circuit. A -0.3V potential generated reduced pyocyanin and ferrocyanide. Ferrocyanide pushes the cell into a reducing condition, allowing pyocyanin to remain reduced to prevent activation of gene expression by a redox sensing gene circuit. | Sulfite removes oxygen from solution allowing pyocyanin to be maintained in a reduced state. A potential of +0.5V generates oxidised pyocyanin and ferricyanide. Ferricyanide pushes the cell into an oxidising condition, allowing pyocyanin to remain oxidised and activate gene expression of a redox sensing gene circuit. A -0.3V potential generated reduced pyocyanin and ferrocyanide. Ferrocyanide pushes the cell into a reducing condition, allowing pyocyanin to remain reduced to prevent activation of gene expression by a redox sensing gene circuit. | ||
− | + | <img src="https://2018.igem.org/File:T--Imperial_College--Naso3structure.png" alt="" width="5%"; >.</p2> | |
</p2> | </p2> | ||
</div> | </div> |
Revision as of 17:57, 17 October 2018
Design
Design Overview
Electrochemical Module Design
![](https://static.igem.org/mediawiki/2018/e/ea/T--Imperial_College--Electrochemicalmodule.png)
Potentiostat:
Electrode or Electrode Array:
Pyocyanin:
![](https://static.igem.org/mediawiki/2018/4/4b/T--Imperial_College--Pyostructure.png)
Ferrocyanide/Ferricyanide:
![](https://static.igem.org/mediawiki/2018/3/38/T--Imperial_College--Ferrostructure.png)
![](https://static.igem.org/mediawiki/2018/1/17/T--Imperial_College--Ferristructure.png)
Sodium Sulfite:
![](https://2018.igem.org/File:T--Imperial_College--Naso3structure.png)
Biological Module Design
![](https://static.igem.org/mediawiki/2018/b/b9/T--Imperial_College--BiologicalModule.png)
SoxR:
pSoxS:
Quinone Pool:
Biological Module Mechanism:
When the Iron-Sulfur centres of SoxR are oxidised by oxidised pyocyanin produced by the electrochemical module, it activates transcription downstream of pSoxS. This allows for electrogenetic induction of any gene or gene circuit downstream of this promoter.PixCell Electrogenetic System Design
![](https://static.igem.org/mediawiki/2018/1/13/T--Imperial_College--0.3SystemPixcell.png)
![](https://static.igem.org/mediawiki/2018/5/58/T--Imperial_College--0.5SystemPixcell.png)