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<h4>Ferrocyanide/Ferricyanide:</h4> | <h4>Ferrocyanide/Ferricyanide:</h4> | ||
− | <p2>These molecules are well known redox mediators, meaning, they alter the redox-state of the cell. When the reduced form (ferricyanide) is present a reducing cellular environment is created, preventing the induction of redox-sensing gene circuits. When the oxidised form (ferrocyanide) is present an oxidising cellular environment is creating, permitting activation of redox-sensing gene circuit. | + | <p2>These molecules are well known redox mediators, meaning, they alter the redox-state of the cell. When the reduced form (ferricyanide) is present a reducing cellular environment is created, preventing the induction of redox-sensing gene circuits. When the oxidised form (ferrocyanide) is present an oxidising cellular environment is creating, permitting activation of redox-sensing gene circuit. </br> |
<img src="https://static.igem.org/mediawiki/2018/3/38/T--Imperial_College--Ferrostructure.png" alt="" width="20%"; >.</p2> | <img src="https://static.igem.org/mediawiki/2018/3/38/T--Imperial_College--Ferrostructure.png" alt="" width="20%"; >.</p2> | ||
<img src="https://static.igem.org/mediawiki/2018/1/17/T--Imperial_College--Ferristructure.png" alt="" width="20%"; >.</p2> | <img src="https://static.igem.org/mediawiki/2018/1/17/T--Imperial_College--Ferristructure.png" alt="" width="20%"; >.</p2> |
Revision as of 18:00, 17 October 2018
Design
Design Overview
Electrochemical Module Design
![](https://static.igem.org/mediawiki/2018/e/ea/T--Imperial_College--Electrochemicalmodule.png)
Potentiostat:
Electrode or Electrode Array:
Pyocyanin:
![](https://static.igem.org/mediawiki/2018/4/4b/T--Imperial_College--Pyostructure.png)
Ferrocyanide/Ferricyanide:
![](https://static.igem.org/mediawiki/2018/3/38/T--Imperial_College--Ferrostructure.png)
![](https://static.igem.org/mediawiki/2018/1/17/T--Imperial_College--Ferristructure.png)
Sodium Sulfite:
![](https://2018.igem.org/File:T--Imperial_College--Naso3structure.png)
Biological Module Design
![](https://static.igem.org/mediawiki/2018/b/b9/T--Imperial_College--BiologicalModule.png)
SoxR:
pSoxS:
Quinone Pool:
Biological Module Mechanism:
When the Iron-Sulfur centres of SoxR are oxidised by oxidised pyocyanin produced by the electrochemical module, it activates transcription downstream of pSoxS. This allows for electrogenetic induction of any gene or gene circuit downstream of this promoter.PixCell Electrogenetic System Design
![](https://static.igem.org/mediawiki/2018/1/13/T--Imperial_College--0.3SystemPixcell.png)
![](https://static.igem.org/mediawiki/2018/5/58/T--Imperial_College--0.5SystemPixcell.png)