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<p2>Oxidised redox molecules oxidise the transcription factor SoxR. This allows it to bind to and initiate transcription from the pSoxS promoter. This allows for the electronic induction of any gene placed downstream of this promoter.</p2> | <p2>Oxidised redox molecules oxidise the transcription factor SoxR. This allows it to bind to and initiate transcription from the pSoxS promoter. This allows for the electronic induction of any gene placed downstream of this promoter.</p2> | ||
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<button class="collapsible">Details</button> | <button class="collapsible">Details</button> |
Latest revision as of 18:48, 17 October 2018
Design
Design Overview
Electrochemical Module Design
![](https://static.igem.org/mediawiki/2018/d/d6/T--Imperial_College--Electrochemicalmodulenew.png)
Potentiostat:
Electrode or Electrode Array:
Pyocyanin:
![](https://static.igem.org/mediawiki/2018/4/4b/T--Imperial_College--Pyostructure.png)
Ferrocyanide/Ferricyanide:
![](https://static.igem.org/mediawiki/2018/3/38/T--Imperial_College--Ferrostructure.png)
![](https://static.igem.org/mediawiki/2018/1/17/T--Imperial_College--Ferristructure.png)
Sodium Sulfite:
![](https://static.igem.org/mediawiki/2018/4/4c/T--Imperial_College--Naso3structure.png)
Biological Module Design
![](https://static.igem.org/mediawiki/2018/6/6d/T--Imperial_College--Biologicalmodulenew.png)
SoxR:
pSoxS:
Quinone Pool:
Biological Module Mechanism:
When the Iron-Sulfur centres of SoxR are oxidised by oxidised pyocyanin produced by the electrochemical module, it activates transcription downstream of pSoxS. This allows for electrogenetic induction of any gene or gene circuit downstream of this promoter.PixCell Electrogenetic System Design
![](https://static.igem.org/mediawiki/2018/1/19/T--Imperial_College--Offstatesystemfinal.png)
![](https://static.igem.org/mediawiki/2018/9/96/T--Imperial_College--Onstatesystem.png)