Difference between revisions of "Team:Tokyo Tech"

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<title>Coli Sapiens</title>
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<meta charset="UTF-8">
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<meta name="viewport" content="width=device-width, initial-scale=1">
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<link rel="stylesheet" href="main.css">
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<style>
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body,h1,h2,h3,h4,h5 {font-family: "Arial", sans-serif}
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body {font-size:16px;}
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    <div class="photo-show">
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<div class="column full_size" >
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    <img src="https://static.igem.org/mediawiki/2017/f/f4/T--TokyoTech--load_1.png" width="600">
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<h1> Welcome to iGEM 2018! </h1>
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    <img src="https://static.igem.org/mediawiki/2017/4/43/T--TokyoTech--load_2.png" width="600">
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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    </div>
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<img src="http://placehold.it/1080x320/c4baba/e4dede">
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<div class="column full_size" >
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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</ul>
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</div>
 
</div>
  
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<!-- Sidebar/menu -->
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<nav class="w3-sidebar w3-red w3-collapse w3-top w3-large w3-padding" style="z-index:3;width:300px;font-weight:bold;" id="mySidebar"><br>
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  <a href="javascript:void(0)" onclick="w3_close()" class="w3-button w3-hide-large w3-display-topleft" style="width:100%;font-size:20px">Close Menu</a>
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  <div class="w3-container" style="margin-top: 20px;margin-bottom: 25px" id="wrap">
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    <img src="https://static.igem.org/mediawiki/2018/a/ad/T--Tokyo_Tech--logo_0629.png" style="width: 100%">
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  </div>
  
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<div class="clear extra_space"></div>
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  <div id="contents" style="z-index: 10">
  
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    <ul class="click_menu_checkbox vertm_down">
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    <li style="margin-bottom: 10px; border: 5px double #fff; border-radius: 10px;">
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    <input type="checkbox" id="vmcb-a" />
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    <label for="vmcb-a"><a href="https://2018.igem.org/Team:Tokyo_Tech">Home</a></label>
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    </li>
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    <li style="margin-bottom: 10px; border: 5px double #fff; border-radius: 10px">
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    <input type="checkbox" id="vmcb-b" />
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    <label for="vmcb-b"><a href="https://2018.igem.org/Team:Tokyo_Tech/Medal">Achievements</a></label>
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    </li>
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    <li style="margin-bottom: 10px; border: 5px double #fff; border-radius: 10px">
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    <input type="checkbox" id="vmcb-c" />
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    <label for="vmcb-c"><a>Project</a></label>
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    <ul>
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      <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Description" class="w3-bar-item w3-button w3-hover-white">Description</a></li>
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      <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Project/Basic_Parts" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Basic Parts</a></li>
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      <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Project/Composite_Parts" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Composite Parts</a></li>
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    </ul>
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    </li>
  
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<div class="column full_size" >
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    <li style="margin-bottom: 10px; border: 5px double #fff; border-radius: 10px">
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<h3> Styling your wiki </h3>
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    <input type="checkbox" id="vmcb-d" />
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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    <label for="vmcb-d"><a>Experiment</a></label>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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    </li>
  
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</div>
 
  
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    <li style="margin-bottom: 10px; text-align: center; border: 5px double #fff; border-radius: 10px">
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    <input type="checkbox" id="vmcb-e" />
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    <label for="vmcb-e"><a href="https://2018.igem.org/Team:Tokyo_Tech/Model">Modelling</a></label>
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    </li>
  
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    <li style="margin-bottom: 10px; text-align: center; border: 5px double #fff; border-radius: 10px">
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    <input type="checkbox" id="vmcb-f" />
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    <label for="vmcb-f"><a>Human Practices</a></label>
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    <ul>
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    <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/HP" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Overview</a></li>
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    <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/HP/Silver" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Silver</a></li>
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    <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/HP/Gold_Integrated" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Gold (Integrated)</a></li>
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    <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Demonstrate" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Demonstrate</a></li>
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    <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Collaborations" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Collaborations</a></li>
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    </ul>
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    </li>
  
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    <li style="margin-bottom: 10px; text-align: center; border: 5px double #fff; border-radius: 10px">
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    <input type="checkbox" id="vmcb-g" />
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    <label for="vmcb-g"><a>About us</a></label>
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    <ul>
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    <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Team" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Team</a></li>
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    <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Attributions" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Attributions</a></li>
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    <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Sponsors" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Sponsors</a></li>
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    </ul>
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    </li>
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    </ul>
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    </div>
  
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<!-- Top menu on small screens -->
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<header class="w3-container w3-top w3-hide-large w3-red w3-xlarge w3-padding">
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  <a href="javascript:void(0)" class="w3-button w3-red w3-margin-right" onclick="w3_open()"><img src="http://www.adultb2b.biz/wp-content/themes/adultb2b%20-%20new/assets/images/menuBtn.png" style="width: 30px"></a>
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  <span style="padding-top: 5px">iGEM Tokyo Tech</span>
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</header>
  
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<div class="w3-overlay w3-hide-large" onclick="w3_close()" style="cursor:pointer" title="close side menu" id="myOverlay"></div>
  
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<div class="column third_size" >
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<!-- PAGE CONTENT! -->
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<div class="w3-main" style="margin-left:340px;margin-right:40px">
  
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<h3> Uploading pictures and files </h3>
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<div class="w3-container" id="contact" style="margin-top:30px"><!-- ページタイトル -->
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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      <h1 class="w3-xxxlarge w3-text-red" style="padding-bottom: 10px;padding-top: 10px" align="center">Project Description</h1>
 +
    </div>
  
 +
<hr>
  
−
<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<div class="w3-container" id="contact" style="margin-top:30px"><!-- ページタイトル -->
 +
      <h1 class="w3-xxxlarge w3-text-red" style="padding-bottom: 10px;padding-top: 10px" align="center">Dengue Virus Detection System with multiple colors</h1>
 +
    </div>
  
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<div class="button_link">
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<hr>
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<a href="https://2018.igem.org/Special:Upload">
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UPLOAD FILES
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</a>
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</div>
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</div>
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  <div class="w3-container" id="mecha" style="margin-top:20px">
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    <h1 class="w3-xxxlarge w3-text-red" style="padding-bottom: 10px;padding-top: 10px"><b>Mechanism</b></h1>
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    <hr style="width:50px;border:5px solid red" class="w3-round">
  
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<div class="column third_size" >
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      <center>
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<h3> Wiki template information </h3>
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      <div style="padding: 10px; margin-bottom: 3px; border: 1px dotted #333333;width: 90%; border-radius: 10px">
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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      <h4 style="text-align: center">Virus detection system with multi-colors</h4>
 +
      <p style="font-size:16px;font-size: 16px; text-indent:1em;padding-top: 15px">
 +
      For 2018 project, we try to develop mosaic-envelope virus that can detect the presence of antibodies in serum that correspond to specific type of virus. This mosaic-envelope virus can detect all the serotypes of dengue viruses and can contribute to versatile dengue virus detection. The experimental procedures are categorized as the following three steps.
 +
      </p>
 +
      <p style="font-size:16px;font-size: 16px; text-indent:1em;padding-top: 15px">
 +
        STEP1: We construct integrated plasmid that codes essential viral proteins (both structural and non-structural) and green fluorescence protein. (Note: If the size is too large, they will be separated.) The envelope (E) genes are derived from 2 or more dengue serotypes so that envelope proteins can be mixed to detect multiple types of antibodies.
 +
      </p>
 +
      <p style="font-size:16px;font-size: 16px; text-indent:1em;padding-top: 15px">
 +
      STEP2: We transduce the plasmid into suitable eukaryotic cells and culture the cells which are successfully transduced. (Drug selection and a series of passage may be needed.) Most of the transduced cells are expected to produce mosaic-envelope virus.
 +
      </p>
 +
      <p style="font-size:16px;font-size: 16px; text-indent:1em;padding-top: 15px">
 +
      <p style="font-size:16px;font-size: 16px; text-indent:1em;padding-top: 15px">
 +
        STEP3: We culture the target mammalian cells that are for checking the infection by mosaic-envelope virus. After mixing the cells and the virus, we measure the fluorescence intensity to check whether the mosaic-envelope virus infected and GFPs are produced or not. As control experiment, we add antibodies that correspond to one of the dengue serotypes to check whether the function of the virus is inhibited or not.
  
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</div>  
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      </p>
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      </p>
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      <div class="w3-xxxlarge" style="padding-bottom: 3px;text-align: center">
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      <figure>
 +
      <img src="https://static.igem.org/mediawiki/2018/4/48/T--Tokyo_Tech--virus_detec_flow_0629.png" style="max-width:80%">
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      <figcaption style="font-size: 16px">Fig. 1 Basic Flow of dengue virus detection system</figcaption>
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      </figure>
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      </div>
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      </div>
  
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<div class="highlight decoration_B_full">
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<h3> Editing your wiki </h3>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<div class="button_link">
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<a href="https://2018.igem.org/wiki/index.php?title=Team:Tokyo_Tech&action=edit">
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EDIT PAGE
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<h3>Tips</h3>
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
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<ul>
 
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
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<li>Be clear about what you are doing and how you plan to do this.</li>
 
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
 
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<li>Have lots of fun! </li>
 
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<div class="w3-container" id="contact" style="margin-top:20px">
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<div class="column third_size">
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<p id="pageTop" style="text-align:right"><a href="#wrap"><img src="https://static.igem.org/mediawiki/2017/0/0d/T--TokyoTech--page_top_2.png" style="width:200px"></a></p>
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<div class="highlight decoration_A_full">
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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</div>
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</div>
 
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<!-- W3.CSS Container -->
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<div class="w3-light-grey w3-container w3-padding-32" style="margin-top:75px;padding-right:58px"><p class="w3-right"><a href="http://96haji.me/" title="W3.CSS" target="_blank" class="w3-hover-opacity">Hajime Fujita with W3.CSS: All Rights Reserved</a></p></div>
  
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<script src="http://ajax.googleapis.com/ajax/libs/jquery/1.11.1/jquery.min.js"></script>
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<script>
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// Script to open and close sidebar
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function w3_open() {
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    document.getElementById("mySidebar").style.display = "block";
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    document.getElementById("myOverlay").style.display = "block";
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}
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function w3_close() {
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    document.getElementById("mySidebar").style.display = "none";
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    document.getElementById("myOverlay").style.display = "none";
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}
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<script>
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function onClick(element) {
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  var captionText = document.getElementById("caption");
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  captionText.innerHTML = element.alt;
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$(function() {
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  $('#wrap').css('display','none');
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$(window).load(function () { //全ての読み込みが完了したら実行
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  $('#loader-bg').delay(900).fadeOut(800);
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  $('#loader').delay(600).fadeOut(300);
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  $('#wrap').css('display', 'block');
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<script>
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//■page topボタン
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$(function(){
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var topBtn=$('#pageTop');
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topBtn.hide();
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//◇ボタンの表示設定
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$(window).scroll(function(){
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  if($(this).scrollTop()>80){
 +
    //---- 画面を80pxスクロールしたら、ボタンを表示する
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    topBtn.fadeIn();
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    //---- 画面が80pxより上なら、ボタンを表示しない
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    topBtn.fadeOut();
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// ◇ボタンをクリックしたら、スクロールして上に戻る
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topBtn.click(function(){
 +
  $('body,html').animate({
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});
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</script>
  
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</html>

Revision as of 04:10, 30 June 2018

<!DOCTYPE html> Coli Sapiens

iGEM Tokyo Tech

Project Description

Dengue Virus Detection System with multiple colors

Mechanism

Virus detection system with multi-colors

For 2018 project, we try to develop mosaic-envelope virus that can detect the presence of antibodies in serum that correspond to specific type of virus. This mosaic-envelope virus can detect all the serotypes of dengue viruses and can contribute to versatile dengue virus detection. The experimental procedures are categorized as the following three steps.

STEP1: We construct integrated plasmid that codes essential viral proteins (both structural and non-structural) and green fluorescence protein. (Note: If the size is too large, they will be separated.) The envelope (E) genes are derived from 2 or more dengue serotypes so that envelope proteins can be mixed to detect multiple types of antibodies.

STEP2: We transduce the plasmid into suitable eukaryotic cells and culture the cells which are successfully transduced. (Drug selection and a series of passage may be needed.) Most of the transduced cells are expected to produce mosaic-envelope virus.

STEP3: We culture the target mammalian cells that are for checking the infection by mosaic-envelope virus. After mixing the cells and the virus, we measure the fluorescence intensity to check whether the mosaic-envelope virus infected and GFPs are produced or not. As control experiment, we add antibodies that correspond to one of the dengue serotypes to check whether the function of the virus is inhibited or not.

Fig. 1 Basic Flow of dengue virus detection system

Hajime Fujita with W3.CSS: All Rights Reserved