|
|
| Line 7: |
Line 7: |
| | <head> | | <head> |
| | <title>Page Title</title> | | <title>Page Title</title> |
| − | </head>
| |
| − | <body>
| |
| | <style> | | <style> |
| − | /*.row {
| |
| − |
| |
| − | width: 25%;
| |
| − | padding-right: 5px;
| |
| − | display: flex;
| |
| − | float: center;
| |
| − | }
| |
| − |
| |
| − | /* Create three equal columns that sits next to each other */
| |
| − | .column {
| |
| − | flex: 50%;
| |
| − | padding: 2px;
| |
| − | display: block;
| |
| − | }*/
| |
| | | | |
| | div { | | div { |
| Line 32: |
Line 16: |
| | .floatRight {width: 25%; float: right; } | | .floatRight {width: 25%; float: right; } |
| | | | |
| − | /*.parallax { | + | </style> |
| − | /* The image used */
| + | |
| − | background-image: url("https://static.igem.org/mediawiki/2018/8/88/T--William_and_Mary--Parts.jpeg");
| + | |
| − |
| + | |
| | | | |
| − | /* Set a specific height */
| |
| − | height: 300px;
| |
| − |
| |
| − |
| |
| − | /* Create the parallax scrolling effect */
| |
| − | background-attachment: fixed;
| |
| − | background-position: top;
| |
| − | background-repeat: no-repeat;
| |
| − | background-size: cover;
| |
| − |
| |
| − |
| |
| − |
| |
| − | }*/
| |
| − |
| |
| − |
| |
| − | </style>
| |
| | </head> | | </head> |
| − | | + | <body> |
| − | <body style="font-size: 15px"> | + | <div style='padding-top: 50px;'></div> |
| − | <br></br>
| + | |
| − | | + | |
| − | <div class='parallax'></div>
| + | |
| − | <center>
| + | |
| − | <div style="padding-left:100px; background-color: ;width:1000px">
| + | |
| − | | + | |
| − | | + | |
| − | <div style='padding-top: 30px;'></div> | + | |
| | | | |
| | <h1 style="color:green;text-align:center;">Best Part Collections</h1> | | <h1 style="color:green;text-align:center;">Best Part Collections</h1> |
| Line 85: |
Line 42: |
| | While our part collection is complete for the purpose of our project, it is also open to addition. A 3G library will be an invaluable tool for other teams also hoping to implement 3G assembly, and thus the library will be open-access for all iGEM teams to contribute to. </div> | | While our part collection is complete for the purpose of our project, it is also open to addition. A 3G library will be an invaluable tool for other teams also hoping to implement 3G assembly, and thus the library will be open-access for all iGEM teams to contribute to. </div> |
| | | | |
| − |
| |
| − | </div>
| |
| − | <div style='padding-top: 20px;'></div>
| |
| − |
| |
| − | <!--<div class="basic parts">
| |
| − | <center>
| |
| − | <div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 20px' ><b>Basic Parts</b></div>
| |
| − | <div style='background: #808080; margin: 0px 14% 20px 14%; height:1px;></div>
| |
| − | <div style='padding-top: 0px;'></div>
| |
| − | <table style=''>
| |
| − |
| |
| − |
| |
| − |
| |
| − | <tr>
| |
| − | <th style='background-color: #65A686;column-width: 50px;'><center>Name</center></th>
| |
| − | <th style='background-color: #65A686;column-width: 200px;'><center>Part ID</center></th>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680500"> K2680500 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>WM_Pad1</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680501"> K2680501 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>WM_Pad2</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680502"> K2680502 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>Prefix BsaI Sticky End A</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680503"> K2680503 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>Suffix BsaI Sticky End B</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680504"> K2680504 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>Prefix BsaI Sticky End B</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680505"> K2680505 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>Prefix BsaI Sticky End C (Abreviated)</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680506"> K2680506 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>Prefix BsaI Sticky End C (Full）</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680507"> K2680507 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>Suffix BsaI Sticky End D</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680508"> K2680508 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>Prefix BsaI Sticky End D</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680509"> K2680509 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>Suffix BsaI Sticky End E</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680510"> K2680510 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>BsaI prefix Spacer</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680511"> K2680511 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>BsaI suffix Spacer</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680512"> K2680512 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>Suffix BsaI Sticky End C</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680513"> K2680513 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>WM_Pad2 Construction primer</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680514"> K2680514 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>WM_Pad1 Construction primer</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680515"> K2680515 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>WM_Pad Insert Amplification primer forward</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680516"> K2680516 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>WM_Pad Insert Amplification primer reverse</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680517"> K2680517 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>WM_Pad Backbone Amplification primer forward</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680518"> K2680518 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>WM_Pad Backbone Amplification primer reverse</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680519"> K2680519 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>pILacO</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680520"> K2680520 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>PcinAM</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680521"> K2680521 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>PlasAM</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680522"> K2680522 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>PluxAM</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680523"> K2680523 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>PsalAM</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680524"> K2680524 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>Plac/ara-1</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680525"> K2680525 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>pBAD</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680526"> K2680526 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>pLsrR</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680527"> K2680527 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>pLsrA</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680527"> K2680527 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>pLsrA</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680528"> K2680528 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>pTlpA</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680529"> K2680529 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>BCD12</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680530"> K2680530 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>B0033m</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680531"> K2680531 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>B0032m</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680532"> K2680532 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>sfGFP</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680533"> K2680533 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>L3S1P13 terminator</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680534"> K2680534 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>T500 terminator</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680535"> K2680535 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>ECK120033736</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680536"> K2680536 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>S. pyogenes tracrRNA terminator</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680541"> K2680541 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>sfYFP</td>
| |
| − | </tr>
| |
| − |
| |
| − | </table>
| |
| − | </center>
| |
| − |
| |
| − |
| |
| − | </div>-->
| |
| − |
| |
| − |
| |
| − | <div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 20px' ><b>3G Parts</b></div>
| |
| − | <div style='background: #808080; margin: 0px 14% 20px 14%; height:1px;></div>
| |
| − | <div style='padding-top: 0px;'></div>
| |
| − |
| |
| − | <div class="floatLeft" alt="ab 3g parts">
| |
| − | <center>
| |
| − | <h4>Promoters</h4>
| |
| − | <table style=' '>
| |
| − |
| |
| − | <tr>
| |
| − | <th style='background-color: #65A686;column-width: 50px;'><center>Name</center></th>
| |
| − | <th style='background-color: #65A686;column-width: 200px;'><center>Part ID</center></th>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680100"> K2680100 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G J23103</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680101"> K2680101 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G J23116</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680102"> K2680102 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G J23107</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680103"> K2680103 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G J23106</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680104"> K2680104 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G J231026</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680105"> K2680105 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G J23100</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680106"> K2680106 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G pLacCIDAR</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680107"> K2680107 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G pTet</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680108"> K2680108 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G plLact</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680109"> K2680109 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G PcinAM</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680110"> K2680110 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G PlasAM</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K26801110"> K2680111 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G PluxAM</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680112"> K2680112 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G PsalAM</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680113"> K2680113 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G J23115</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680114"> K2680114 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G J23101</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680115"> K2680115 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G pLuxR-pR</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680116"> K2680116 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G pLac/ara-1</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680117"> K2680117 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G pBad</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680118"> K2680118 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G T7</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680119"> K2680119 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G CI repressible promoter</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680121"> K2680121 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G pLsrR</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680122"> K2680122 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G pLsrA</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680123"> K2680123 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G pTlpA</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680124"> K2680124 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G pTlpAr</td>
| |
| − | </tr>
| |
| − |
| |
| − | </table>
| |
| − | </center>
| |
| − | </div>
| |
| − |
| |
| − | <div class="floatLeft" alt="bc 3g parts">
| |
| − | <center>
| |
| − | <h4>5' UnTranslated Regions</h4>
| |
| − | <table style=''>
| |
| − |
| |
| − | <tr>
| |
| − | <th style='background-color: #65A686;column-width: 50px;'><center>Name</center></th>
| |
| − | <th style='background-color: #65A686;column-width: 200px;'><center>Part ID</center></th>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680200"> K2680200 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G BCD8</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680201"> K2680201 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G BCD12</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680202"> K2680202 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G BCD2</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680203"> K2680203 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G B0033m</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680204"> K2680204 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G B0032m</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680205"> K2680205 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G B0034m</td>
| |
| − | </tr>
| |
| − |
| |
| − | </table>
| |
| − | </center>
| |
| − | </div>
| |
| − |
| |
| − | <div class="floatRight" alt="cd 3g parts">
| |
| − | <center>
| |
| − | <h4>Coding Sequences</h4>
| |
| − | <table style=''>
| |
| − |
| |
| − | <tr>
| |
| − | <th style='background-color: #65A686;column-width: 50px;'><center>Name</center></th>
| |
| − | <th style='background-color: #65A686;column-width: 200px;'><center>Part ID</center></th>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680250"> K2680250 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G sfGFP</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680251"> K2680251 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G mScarlet-I</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680252"> K2680252 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G mScarlet-I pdt#3</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680253"> K2680253 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G mScarlet-I pdt#3a</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680254"> K2680254 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G mScarlet-I pdt#3b</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680255"> K2680255 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G mScarlet-I pdt#3d</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680256"> K2680256 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G mScarlet-I pdt#3e</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680257"> K2680257 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G LacIAM</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680258"> K2680258 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G LacI-ssrA</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680259"> K2680259 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G TetR-ssrA</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680260"> K2680260 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G eBFP2</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680261"> K2680261 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G sfYFP</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680262"> K2680262 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G KanR</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680263"> K2680263 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G AraC-ssrA</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680265"> K2680265 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G sfCFP-pdt3</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680266"> K2680266 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G RFP</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680267"> K2680267 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G GFP</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680268"> K2680268 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G YFP</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680269"> K2680269 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G cre Recombinase</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680270"> K2680270 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G deCFP</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680272"> K2680272 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G deGFP</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680273"> K2680273 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G lambda-cI</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680274"> K2680274 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G deGFP</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680275"> K2680275 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G tsLambda-cI</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680276"> K2680276 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G mf-Lon</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680277"> K2680277 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G mf-Lon SsrA</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680278"> K2680278 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G LsrR</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680279"> K2680279 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G TlpA39</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680280"> K2680280 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G Phage 186 integrase</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680281"> K2680281 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G LsRK</td>
| |
| − | </tr>
| |
| − |
| |
| − | </table>
| |
| − | </center>
| |
| − | </div>
| |
| − |
| |
| − | <div class="floatRight" alt="de 3g parts">
| |
| − | <center>
| |
| − | <h4>Terminators</h4>
| |
| − | <table style=''>
| |
| − |
| |
| − | <tr>
| |
| − | <th style='background-color: #65A686;column-width: 70px;'><center>Name</center></th>
| |
| − | <th style='background-color: #65A686;column-width: 200px;'><center>Part ID</center></th>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680400"> K2680400 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G spy terminator</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680401"> K2680401 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G thrL terminator</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680402"> K2680402 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G L3S1P13 terminator</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680403"> K2680403 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G T500_noGap, short attachment (T11)</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680404"> K2680404 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G B0015</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680405"> K2680405 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G ECK120033736</td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
| |
| − | <td style='background-color: #A2CBAB;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2680406"> K2680406 </a></td>
| |
| − | <td style='background-color: #96B69C;column-width: 200px; font-size: 15px;'>3G S. pyogenes tracrRNA terminator</td>
| |
| − | </tr>
| |
| − |
| |
| − | </table>
| |
| − | </center>
| |
| − | </div>
| |
| − | </div>
| |
| | | | |
| | </body> | | </body> |
In order to determine the appropriate expression levels of various component genes, our project required us to create and test many circuit variants.
To efficiently design and clone circuits containing multiple transcriptional units, we implemented a recently designed method of circuit construction called 3G Assembly, which enables quick and modular cloning of circuits. Furthermore, “mixed” 3G Assembly can be used to construct and test multiple variants of a given circuit. This allows teams to build, test and subsequently clone a vast number of circuit variants in a single day.
Since we anticipate this method to be extremely valuable to future iGEM teams, we submitted approximately 70 parts in a 3G compatible format. These parts include some of the most commonly used basic parts used by iGEM teams, as well as basic parts from our project and parts from our collaborators at UVA.
Our 3G part library consists of 4 categories: Promoter, 5’ UTR (UnTranslated Regions), Coding sequence and Terminator. Most of the variants in each category are used for tuning the relative expression level between the reporter (mScarlet) and the protease (mf-Lon). We can also easily modify degradation rate by switching the degradation tag in mScarlet, changing the strength of the promoter regulating mf-Lon, or adding a degradation tag to mf-Lon (a ssrA tag).
In our collection of parts, we also incorporated the ts-CI heat inducible system and TlpA heat inducible system. To use these systems, we cloned the corresponding promoter and repressor sequences needed into our standard backbone. Using these heat inducible parts, we also designed a wide variety of heat inducible circuits. Their circuits are a convenient tool for any team in the future that are using 3G methods and are pursuing this induction system.
While our part collection is complete for the purpose of our project, it is also open to addition. A 3G library will be an invaluable tool for other teams also hoping to implement 3G assembly, and thus the library will be open-access for all iGEM teams to contribute to.
