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Latest revision as of 19:42, 17 October 2018

Parts

Our improved parts can be accessed here.


Name Type Description
BBa_K2808000 DNA N-acetyl glucosamine-binding protein A (gbpA) gene for the detection of Vibrio cholera
The gbpA gene in Vibrio cholerae codes for the N-acetyl glucosamine-binding protein A (GbpA), which is a chitin-binding protein involved in the attachment of V. cholerae to environmental chitin surfaces and human intestinal cells. The gbpA gene has been found to be consistently present and highly conserved in V. cholerae. GbpA has been used as a target gene for the species-specific detection of V. cholerae O1, O139, Non-O1/Non-O139.
BBa_K2808001 DNA N-benzoylglycine amidohydrolase (hippuricase) (hipO) gene for the detection of Campylobacter jejuni
hipO gene codes for N-benzoylglycine amidohydrolase (hippuricase) in Campylobacter jejuni. Due its specificity to the organism and the lack of toxicity, various research projects have used this gene for the detection of C. jejuni through techniques such as PCR.
BBa_K2808002 DNA Invasion protein (invA) gene for the detection of Salmonella typhimurium
The invA gene is involved in the invasion of the cells of the intestinal epithelium and could be involved in the translocation of the InvE protein in Salmonella typhimurium. Due its specificity to the organism and the lack of toxicity, various research projects have used this gene for the detection of S. typhimurium.
BBa_K2808003 DNA lmo0773 alcohol dehydrogenase gene for the detection of Listeria monocytogenes
The lmo0773 alcohol dehydrogenase is involved in oxidoreductase activity. Due its specificity to the organism and the lack of toxicity, various research projects have used this gene for the detection of Listeria monocytogenes.
BBa_K2808004 Primer gbpA F3 2
BBa_K2808005 Primer gbpA B3 1
BBa_K2808006 Primer Hipo PCR forward 1
BBa_K2808007 Primer Hipo PCR reverse 1
BBa_K2808008 Primer Hipo PCR forward 2
BBa_K2808009 Primer Hipo PCR reverse 2
BBa_K2808010 Primer PCR forward Salmonella 1
BBa_K2808010 Primer PCR forward Salmonella 1
BBa_K2808011 Primer PCR reverse Salmonella 2
BBa_K2808012 Primer PCR forward Listeria
BBa_K2808013 Primer lmo0733 B3 2
BBa_K2808014 Primer gbpA F3 1
BBa_K2808015 Primer gbpA B3 1
BBa_K2808016 Primer gbpA FIP 1
BBa_K2808017 Primer gbpA BIP 1
BBa_K2808018 Primer hipO F3
BBa_K2808019 Primer hipO B3
BBa_K2808020 Primer hipO FIP
BBa_K2808021 DNA hipO BIP
BBa_K2808022 Primer invA F3 1
BBa_K2808023 Primer invA B3 1
BBa_K2808024 Primer invA FIP 1
BBa_K2808025 Primer invA BIP 1
BBa_K2808026 Primer lmo0733 F3 2
BBa_K2808027 Primer lmo0733 B3 2
BBa_K2808028 Primer lmo0733 FIP 2
BBa_K2808029 Primer lmo0733 BIP 2
BBa_K2808030 Primer gbpA RPA F2
BBa_K2808031 Primer gbpA RPA R2
BBa_K2808032 Primer invA RPA F1
BBa_K2808033 Primer invA RPA R1
BBa_K2808034 Primer lmo0733 RPA F1
BBa_K2808035 Primer lmo0733 RPA R1


BioBricks PCR amplification


Figure: Agarose gel electrophoresis showing PCR amplification of: gbpA BioBrick BBA_K2808000; hipO BBA_K2808001; invA BBA_K2808002; lmo0733 BBA_K2808003. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) negative control for gbpA BioBrick; (Lane 4) hipO BioBrick; (Lane 5) negative control for hipO BioBrick; (Lane 6) invA BioBrick; (Lane 7) negative control for invA BioBrick; (Lane 8) lmo0733 BioBrick; (Lane 9) negative control for lmo0733 BioBrick. Lanes 2, 4, 6, and 8 show bands around 1019 bp, 203 bp, 818 bp, and 403 bp respectively as expected for the PCR products of the gbpA, hipO, invA, and lmo0733 BioBricks respectively.


BioBricks double digestion reaction


Figure: Agarose gel electrophoresis showing double digestion of the BioBricks: gbpA BBA_K2808000; hipO BBA_K2808001; invA BBA_K2808002; lmo0733 BBA_K2808003 with EcoRI and PstI. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) gbpA BioBrick digested with EcoRI and PstI. (Lane 4) hipO BioBrick; (Lane 5) hipO BioBrick digested with EcoRI and PstI; (Lane 6) invA BioBrick; (Lane 7) invA BioBrick digested with EcoRI and PstI; (Lane 8) lmo0733 BioBrick; (Lane 9) lmo0733 BioBrick digested with EcoRI and PstI. The lengths of the pSB1C3 backbone, gbpA gene, hipO gene, invA gene, and lmo0733 genes are 2070 bp, 1458 bp, 1152 bp, 2065 bp, and 510 bp respectively.


gbpA BioBrick double digestion reaction


Figure: Agarose gel electrophoresis showing double digestion of the gbpA BioBrick BBA_K2808000 (gbpA gene (1458 bp) inserted into the pSB1C3 backbone (2070 bp)) with EcoRI and PstI. (Lane 1) 500 bp DNA ladder (Bio-Rad); (Lane 2) gbpA BioBrick; (Lane 3) gbpA BioBrick digested with EcoRI and PstI.




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