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| | The toxicity of copper ions on the cell is well characterized (Ning <i>et al.</i>, 2015) but for important parts of this project like the <a href="https://2018.igem.org/Team:Bielefeld-CeBiTec/Hardware">crossflow reactor</a> we needed to know the exact point of time when our cells will die to achieve highest possible yields of copper. | | The toxicity of copper ions on the cell is well characterized (Ning <i>et al.</i>, 2015) but for important parts of this project like the <a href="https://2018.igem.org/Team:Bielefeld-CeBiTec/Hardware">crossflow reactor</a> we needed to know the exact point of time when our cells will die to achieve highest possible yields of copper. |
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| − | The residence time should not exceed the rate of dying and cell lysis in <a href="https://2018.igem.org/Team:Bielefeld-CeBiTec/Hardware">the system</a>. If cell lysis kicks in copper gets released back again into the substrate media and the yield minimizes. The modeling started with the copper uptake in our cells containing the BioBrick BBa_K2638204, which expresses <i>oprC</i> un<script>der pAraBAD (<a href="https://2018.igem.org/Team:Bielefeld-CeBiTec/Hardware">BBa_I0500)</a>) control and induction at 1.0 % arabinose in. The toxicity is calculated for a single cell.</div><div class="article"> | + | The residence time should not exceed the rate of dying and cell lysis in <a href="https://2018.igem.org/Team:Bielefeld-CeBiTec/Hardware">the system</a>. If cell lysis kicks in copper gets released back again into the substrate media and the yield minimizes. The modeling started with the <script> copper uptake in our cells containing the BioBrick BBa_K2638204, which expresses <i>oprC</i> un<script>der pAraBAD (<a href="https://2018.igem.org/Team:Bielefeld-CeBiTec/Hardware">BBa_I0500)</a>) control and induction at 1.0 % arabinose in. The toxicity is calculated for a single cell.</div><div class="article"> |
| | The first step was to calculate the rate of expression of <i>oprC</i>. Therefore the characterization of <a href="https://2018.igem.org/Team:Bielefeld-CeBiTec/Hardware">BBa_I0500)</a> of <a href="https://2011.igem.org/Team:Groningen/project_characterisation_promoters_pbad">Groningen</a> was used to calculate the expression speed. At 1.0 % arabinose induction a raise of fluorescence of approx. <i>ΔF</i> = 82,000 within of t = 36,000 s was measurable. | | The first step was to calculate the rate of expression of <i>oprC</i>. Therefore the characterization of <a href="https://2018.igem.org/Team:Bielefeld-CeBiTec/Hardware">BBa_I0500)</a> of <a href="https://2011.igem.org/Team:Groningen/project_characterisation_promoters_pbad">Groningen</a> was used to calculate the expression speed. At 1.0 % arabinose induction a raise of fluorescence of approx. <i>ΔF</i> = 82,000 within of t = 36,000 s was measurable. |
| | The conversion from fluorescence units to concentration in mol/L was calculated as <i>k</i> = 2.5 * 10<sup>-6</sup> mol*L<sup>-1</sup> (Furtado and Henry, 2002) and the volume of the used capillaries was <i>V</i> = 3.14 * 10<sup>-9</sup> L The rate of protein expression with 1.0 % arabinose is: | | The conversion from fluorescence units to concentration in mol/L was calculated as <i>k</i> = 2.5 * 10<sup>-6</sup> mol*L<sup>-1</sup> (Furtado and Henry, 2002) and the volume of the used capillaries was <i>V</i> = 3.14 * 10<sup>-9</sup> L The rate of protein expression with 1.0 % arabinose is: |
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