Difference between revisions of "Team:Imperial College/Part Collection"

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<h3> Part Collection </h3>
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</br><h3> PixCell Library</h3>
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<h1> Part Collection </h1>
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<p>The PixCell library is the first ever library of electrogenetic parts. It contains 5 SoxR transcription factors and 8 pSoxS promoters capable of providing electronic gene induction. Parts were sourced from bacterial homologues or created by mutagenesis. Transcription factors and promoters were codon optimised for E. coli and promoters were engineered to be unidirectional, have upstream terminators for easy assembly, and have downstream ribozymes to reduce context dependency. All part combinations were characterised, exhibiting a range of RFU values with  a tunable orthogonal pair being identified. One mutant promoter we designed provided transcriptional repression, further increasing the complexity of electrogenetic circuits that can be constructed. All parts have been submitted in a BioBrick, BASIC and Golden Gate compatible format. Library parts were used to construct an electrogenetic biocontainment device whilst the system’s ability to sense oxidative stress offers extended-uses in various therapeutic, diagnostic and environmental applications.  <a href="http://parts.igem.org/Part:BBa_K2862010"> Please follow the link to our registry parts page in which our part collection characterization data is shown </a>
 
<p>The PixCell library is the first ever library of electrogenetic parts. It contains 5 SoxR transcription factors and 8 pSoxS promoters capable of providing electronic gene induction. Parts were sourced from bacterial homologues or created by mutagenesis. Transcription factors and promoters were codon optimised for E. coli and promoters were engineered to be unidirectional, have upstream terminators for easy assembly, and have downstream ribozymes to reduce context dependency. All part combinations were characterised, exhibiting a range of RFU values with  a tunable orthogonal pair being identified. One mutant promoter we designed provided transcriptional repression, further increasing the complexity of electrogenetic circuits that can be constructed. All parts have been submitted in a BioBrick, BASIC and Golden Gate compatible format. Library parts were used to construct an electrogenetic biocontainment device whilst the system’s ability to sense oxidative stress offers extended-uses in various therapeutic, diagnostic and environmental applications.  <a href="http://parts.igem.org/Part:BBa_K2862010"> Please follow the link to our registry parts page in which our part collection characterization data is shown </a>
 
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<h3>Note</h3>
 
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<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
 
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<h3>Best Part Collection Special Prize</h3>
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<p>To be eligible for this award, these parts must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a collection of parts you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part numbers to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.</p>
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Revision as of 03:41, 18 October 2018





Part Collection


PixCell Library

The PixCell library is the first ever library of electrogenetic parts. It contains 5 SoxR transcription factors and 8 pSoxS promoters capable of providing electronic gene induction. Parts were sourced from bacterial homologues or created by mutagenesis. Transcription factors and promoters were codon optimised for E. coli and promoters were engineered to be unidirectional, have upstream terminators for easy assembly, and have downstream ribozymes to reduce context dependency. All part combinations were characterised, exhibiting a range of RFU values with a tunable orthogonal pair being identified. One mutant promoter we designed provided transcriptional repression, further increasing the complexity of electrogenetic circuits that can be constructed. All parts have been submitted in a BioBrick, BASIC and Golden Gate compatible format. Library parts were used to construct an electrogenetic biocontainment device whilst the system’s ability to sense oxidative stress offers extended-uses in various therapeutic, diagnostic and environmental applications. Please follow the link to our registry parts page in which our part collection characterization data is shown