Difference between revisions of "Team:Stanford-Brown-RISD/InterLab"
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| + | <h1> Calibration: 3 Experiments </h1> | ||
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| + | <h3> 1. OD600 reference point </h3> | ||
| + | <p> The purpose of the first calibration is to establish a conversion from “absorbance at 600nm” (Abs600) data in our 96 well plates into an “optical density at 600nm” (OD600) measurement, as would be obtained in a standard spectrophotometer. “Such conversion is necessary because plate reader measurements of absorbance are volume dependent; the depth of the fluid in the well defines the path length of the light passing through the sample, which can vary slightly from well to well.” | ||
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| + | To do so, we first measured the absorbance values of LUDOX-S30 versus deionized H2O. Reading the LUDOX-S30 absorbance value from our spectrophotometer, we subtract the dH2O absorbance to get a corrected absorbance value. Then, the LUDOX-S30 OD600 from iGEM’s reference spectrophotometer is divided by our corrected absorbance value to give a standardized conversion factor. </p> | ||
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Revision as of 04:38, 31 July 2018
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