Catherinepan (Talk | contribs) |
Catherinepan (Talk | contribs) |
||
Line 20: | Line 20: | ||
-webkit-text-stroke: 0.5px; | -webkit-text-stroke: 0.5px; | ||
font-size: 17px; | font-size: 17px; | ||
− | + | padding-left: 60px; | |
− | + | padding-right: 70px;">Dividing metabolism amongst microbial communities has shown huge potential for the large-scale production of chemical products. Unfortunately, optimizing the population dynamics of the individual strain modules remains a challenge (Jones & Wang, 2018). Our goal is to improve the production of naringenin and its pharmaceutically significant derivatives, which have anti-cancer and anti-inflammatory properties, by distributing the synthesis of a naringenin derivative between two E. coli strains and optimizing their relative proportions in co-culture. One strain will produce naringenin from glucose and the second strain will create the naringenin derivative. We will regulate the ratio of the two strains using a biosensor and a toehold switch. This will couple cell growth with the concentration of naringenin, allowing the co-culture to self-optimize and increase naringenin production. Using our system, we will have demonstrated a novel way to optimize microbial polycultures for the synthesis of metabolically complex compounds.</p> | |
</body> | </body> | ||
</html> | </html> |
Revision as of 02:17, 20 August 2018
UBC iGEM 2018: Distributed Metabolic Pathway of Naringenin
Dividing metabolism amongst microbial communities has shown huge potential for the large-scale production of chemical products. Unfortunately, optimizing the population dynamics of the individual strain modules remains a challenge (Jones & Wang, 2018). Our goal is to improve the production of naringenin and its pharmaceutically significant derivatives, which have anti-cancer and anti-inflammatory properties, by distributing the synthesis of a naringenin derivative between two E. coli strains and optimizing their relative proportions in co-culture. One strain will produce naringenin from glucose and the second strain will create the naringenin derivative. We will regulate the ratio of the two strains using a biosensor and a toehold switch. This will couple cell growth with the concentration of naringenin, allowing the co-culture to self-optimize and increase naringenin production. Using our system, we will have demonstrated a novel way to optimize microbial polycultures for the synthesis of metabolically complex compounds.